LOCUS       sequence-452358-       15106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length 
            human LINE-1 in human cells (codon optimized ORFeus-Hs), with a 
            C-terminal 3xFlag tag on ORF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15106)
  AUTHORS   Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, 
            Sanchez R, Miller BD, Di Stefano LH, Ruiz FX, Hancock M, Isik E, 
            Mendez-Dorantes C, Walpole T, Nichols C, Wan P, Riento K, Halls-Kass
            R, Augustin M, Lammens A, Jestel A, Upla P, Xibinaku K, Congreve S, 
            Hennink M, Rogala KB, Schneider AM, Fairman JE, Christensen SM, 
            Desrosiers B, Bisacchi GS, Saunders OL, Hafeez N, Miao W, Kapeller 
            R, Zaller DM, Sali A, Weichenrieder O, Burns KH, Gotte M, Rout MP, 
            Arnold E, Greenbaum BD, Romero DL, LaCava J, Taylor MS
  TITLE     Structures, functions and adaptations of the human LINE-1 ORF2 
            protein.
  JOURNAL   Nature. 2024 Feb;626(7997):194-206. doi: 10.1038/s41586-023-06947-z.
            Epub 2023 Dec 14.
  PUBMED    38096902
REFERENCE   2  (bases 1 to 15106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06947-z"; journalName: "Nature"; date: 
            "2024-02"; volume: "626"; issue: "7997"; pages: "194-206"
COMMENT     Sequence Label: pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag...
FEATURES             Location/Qualifiers
     source          1..15106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      670..679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5605..5628
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5629..5694
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    5904..6038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5928..5947)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5982..6001
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6181..6203)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6767..8556
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(11189..11207)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11275..11379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11380..11448,11449..12240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11598..11617)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12411..12999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12900..12919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        13194..13693
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13229..13252)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     13626..13645
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             13733..14332
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(13733..13752)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     14229..14249
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgcgtttaaa
      661 cttaattaag ccaccatggg caagaagcag aaccgcaaga ccggcaacag caagacccag
      721 agcgccagcc ccccccccaa ggagcgcagc agcagccccg ccaccgagca gagctggatg
      781 gagaacgact tcgacgagct gcgcgaggag ggcttccgcc gcagcaacta cagcgagctg
      841 cgcgaggaca tccagaccaa gggcaaggag gtggagaact tcgagaagaa cctggaggag
      901 tgcatcaccc gcatcaccaa caccgagaag tgcctgaagg agctgatgga gctgaagacc
      961 aaggcccgcg agctgcgcga ggagtgccgc agcctgcgca gccgctgcga ccaattggag
     1021 gagcgcgtga gcgccatgga ggacgagatg aacgagatga agcgcgaggg caagttccgc
     1081 gagaagcgca tcaagcgcaa cgagcagagc ctgcaggaga tctgggacta cgtgaagcgc
     1141 cccaacctgc gcctgatcgg cgtgcccgag agcgacgtgg agaacggcac caagctggag
     1201 aacaccctgc aggacatcat ccaggagaac ttccccaacc tggcccgcca ggccaacgtg
     1261 cagatccagg agatccagcg caccccccag cgctacagca gccgccgcgc caccccccgc
     1321 cacatcatcg tgcgcttcac caaggtggag atgaaggaga agatgctgcg cgccgcccgc
     1381 gagaagggcc gcgtgaccct gaagggcaag cccatccgtc tcaccgccga cctgagcgcc
     1441 gagaccctgc aggcccgccg cgagtggggc cccatcttca acatcctgaa ggagaagaac
     1501 ttccagcccc gcatcagcta ccccgccaag ctgagcttca tcagcgaggg cgagatcaag
     1561 tacttcatcg acaagcagat gctgcgcgac ttcgtgacca cccgccccgc cctgaaggag
     1621 ctgctgaagg aggccctgaa catggagcgc aacaaccgct accagcccct gcagaaccac
     1681 gccaagatgt aaagaccatc gagactagga agaaactgca tcaactaatg agcaaaatca
     1741 ggcgcgccca tcataatgac cggcagcaac agccacatca ccatcctgac cctgaacatc
     1801 aacggcctga acagcgccat caagcgccac cgcctggcca gctggatcaa gagccaggac
     1861 cccagcgtgt gctgcatcca ggagacccac ctgacctgcc gcgacaccca ccgcctgaag
     1921 atcaagggct ggcgcaagat ctaccaggcc aacggcaagc agaagaaggc cggcgtggcc
     1981 atcctggtga gcgacaagac cgacttcaag cccaccaaga tcaagcgcga caaggagggc
     2041 cactacatca tggtgaaggg cagcatccag caggaggagc tgaccatcct gaacatctac
     2101 gcccccaaca ccggtgcccc ccgcttcatc aagcaggtgc tgagcgacct gcagcgcgac
     2161 ctggacagcc acaccctgat catgggcgac ttcaacaccc ccctgagcac cctggaccgc
     2221 agcacccgcc agaaggtgaa caaggacacc caggagctga acagcgccct gcaccaggcc
     2281 gacctgatcg acatctaccg caccctgcac cccaagagca ccgagtacac cttcttcagc
     2341 gccccccacc acacctacag caagatcgac cacatcgtgg gcagcaaggc cctgctgagc
     2401 aagtgcaagc gcaccgagat catcaccaac tacctgagcg accacagcgc catcaagctg
     2461 gagctgcgca tcaagaacct gacccagagc cgcagcacca cctggaagct gaacaacctg
     2521 ctgctgaacg actactgggt gcacaacgag atgaaggccg agatcaagat gttcttcgaa
     2581 accaacgaga acaaggacac cacctaccag aacctgtggg acgccttcaa ggccgtgtgc
     2641 cgcggcaagt tcatcgccct gaacgcctac aagcgcaagc aggagcgcag caagatcgac
     2701 accctgacca gccagctgaa ggagctggag aagcaggagc agacccacag caaggccagc
     2761 cgccgccagg agatcaccaa gatccgcgcc gagctgaagg agatcgagac ccagaagacc
     2821 ctgcagaaga tcaacgagtc gcgaagctgg ttcttcgagc gcatcaacaa gatcgaccgc
     2881 cccctggccc gcctgatcaa gaagaagcgc gagaagaacc agatcgacac catcaagaac
     2941 gacaagggcg acatcaccac cgaccccacc gagatccaga ccaccatccg cgagtactac
     3001 aagcacctgt acgccaacaa gctggagaac ctggaggaga tggacacctt cctggacacc
     3061 tacaccctgc cccgcctgaa ccaggaggag gtggagagcc tgaaccgccc catcaccggc
     3121 agcgagatcg tggccatcat caacagcctg cccaccaaga agagccccgg ccccgacggc
     3181 ttcaccgccg agttctacca gcgctacaag gaggagctgg tgcccttcct gctgaagctg
     3241 ttccaaagca tcgagaagga gggcatcctg cccaacagct tctacgaggc cagcatcatc
     3301 ctgatcccca agcccggccg cgacaccacc aagaaggaga acttccgccc catcagcctg
     3361 atgaacatcg acgccaagat cctgaacaag atcctggcca accgcatcca gcagcacatc
     3421 aagaagctga tccaccacga ccaggtgggc ttcatccccg ggatgcaggg ctggttcaac
     3481 atccgcaaga gcatcaacgt gatccagcac atcaaccgcg ccaaggacaa gaaccacatg
     3541 atcatcagca tcgacgccga gaaggccttc gacaagatcc agcagccctt catgctgaag
     3601 accctgaaca agctgggcat cgacggcacc tacttcaaga tcatccgcgc catctacgac
     3661 aagcccaccg ccaacatcat cctgaacggc cagaagctgg aggccttccc cctgaagacc
     3721 ggcacgcgtc agggctgccc cctgagcccc ctgctgttca acatcgtgct ggaggtgctg
     3781 gcccgcgcca tccgccagga gaaggagatc aagggcatcc agctgggcaa ggaggaggtg
     3841 aagctgagcc tgttcgccta cgacatgatc gtgtacctgg agaaccccat cgtgagcgcc
     3901 cagaacctgc tgaagctgat cagcaacttc agcaaggtga gcggctacaa gatcaacgtg
     3961 cagaagagcc aggccttcct gtacaccaac aaccgccaga ccgagagcca gatcatgggc
     4021 gagctgccct tcaccatcgc tagcaagcgc atcaagtacc tgggcatcca gctgacccgc
     4081 gacgtgaagg acctgttcaa ggagaactac aagcccctgc tgaaggagat caaggaggag
     4141 accaacaagt ggaagaacat cccctgcagc tgggtgggcc gcatcaacat cgtgaagatg
     4201 gccatcctgc ccaaggtgat ctaccgcttc aacgccatcc ccatcaagct gcccatgacc
     4261 ttcttcaccg agctggagaa gaccaccctg aagttcatct ggaaccagaa gcgcgcccgc
     4321 atcgccaaga gcatcctgag ccagaagaac aaggccggcg gcatcaccct gcccgacttc
     4381 aagctgtact acaaggccac cgtgaccaag accgcctggt actggtacca gaaccgcgat
     4441 atcgaccagt ggaaccgcac cgagcccagc gagatcatgc cccacatcta caactacctg
     4501 atcttcgaca agcccgagaa gaacaagcag tggggcaagg acagcctgtt caacaagtgg
     4561 tgctgggaga actggctggc catctgccgc aagctgaagc tggacccctt cctgaccccc
     4621 tacaccaaga tcaacagccg ctggatcaag gacctgaacg tgaagcccaa gaccatcaag
     4681 accctggagg agaacctggg catcaccatc caggacatcg gcgtgggcaa ggacttcatg
     4741 agcaagaccc ccaaggccat ggccaccaag gacaagatcg acaagtggga cctgatcaag
     4801 ctgaagagct tctgcaccgc caaggagacc accatccgcg tgaaccgcca gcccaccacc
     4861 tgggagaaga tcttcgccac ctacagcagc gacaagggcc tgatcagccg catctacaac
     4921 gagctgaagc agatctacaa gaagaagacc aacaacccca tcaagaagtg ggccaaggac
     4981 atgaaccgcc acttcagcaa ggaggacatc tacgccgcca agaagcatat gaagaagtgc
     5041 agcagcagcc tggccatccg cgagatgcag atcaagacca ccatgcgcta ccacctgacc
     5101 cccgtgcgca tggccatcat caagaagagc ggcaacaacc gctgctggcg cggctgcggc
     5161 gagatcggca ccctgctgca ctgctggtgg gactgcaagc tggtgcagcc cctgtggaag
     5221 agcgtgtggc gcttcctgcg cgacctggag ctggagatcc ccttcgaccc cgccatcccc
     5281 ctgctgggca tctaccccaa cgagtacaag agctgctgct acaaggacac ctgcacccgc
     5341 atgttcatcg ccgccctgtt caccatcgcc aagacctgga accagcccaa gtgccccacc
     5401 atgatcgatt ggatcaagaa gatgtggcac atctacacca tggagtacta cgccgccatc
     5461 aagaacgacg agttcatcag cttcgtgggc acctggatga agctggagac catcatcctg
     5521 agcaagctga gccaggagca gaagaccaag caccgcatct tcagcctgat cggcggcaac
     5581 ggcggcggca gcggcggcgg cagcctggaa gttctgttcc aggggcccga ctacaaagac
     5641 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagtagaca
     5701 atgagatcac atggacacag gaaggggaat atcacactct ggggactgtg gtggggtcgg
     5761 gggagggggg agggatagca ttgggagata tacctaatgc tagatgacac attagtgggt
     5821 gcagcgcacc agcatggcac atgtatacat atgtaactaa cctgcacaat gtgcacatgt
     5881 accctaaaac ttagagtata atggatccag acatgataag atacattgat gagtttggac
     5941 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6001 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     6061 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     6121 aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa
     6181 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     6241 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag
     6301 gtcgatcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     6361 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     6421 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     6481 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     6541 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     6601 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     6661 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     6721 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     6781 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     6841 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     6901 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     6961 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     7021 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     7081 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     7141 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     7201 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     7261 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     7321 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     7381 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     7441 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     7501 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     7561 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     7621 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     7681 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     7741 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     7801 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     7861 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     7921 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     7981 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     8041 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     8101 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     8161 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     8221 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     8281 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     8341 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     8401 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca
     8461 gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca
     8521 agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga
     8581 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
     8641 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
     8701 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
     8761 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
     8821 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
     8881 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
     8941 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
     9001 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
     9061 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
     9121 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
     9181 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
     9241 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
     9301 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
     9361 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
     9421 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
     9481 ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa
     9541 aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg
     9601 gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc
     9661 cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac
     9721 cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc
     9781 cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cctgcccctc
     9841 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tccctcctgc tcctgcccct
     9901 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc
     9961 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
    10021 gcccctcctg ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct
    10081 cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct
    10141 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
    10201 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc
    10261 cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
    10321 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
    10381 cctgcccctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
    10441 gctcctgccc ctcctgctcc tgcccctccc gctcctgctc ctgctcctgt tccaccgtgg
    10501 gtccctttgc agccaatgca acttggacgt ttttggggtc tccggacacc atctctatgt
    10561 cttggccctg atcctgagcc gcccggggct cctggtcttc cgcctcctcg tcctcgtcct
    10621 cttccccgtc ctcgtccatg gttatcaccc cctcttcttt gaggtccact gccgccggag
    10681 ccttctggtc cagatgtgtc tcccttctct cctaggccat ttccaggtcc tgtacctggc
    10741 ccctcgtcag acatgattca cactaaaaga gatcaataga catctttatt agacgacgct
    10801 cagtgaatac agggagtgca gactcctgcc ccctccaaca gcccccccac cctcatcccc
    10861 ttcatggtcg ctgtcagaca gatccaggtc tgaaaattcc ccatcctccg aaccatcctc
    10921 gtcctcatca ccaattactc gcagcccgga aaactcccgc tgaacatcct caagatttgc
    10981 gtcctgagcc tcaagccagg cctcaaattc ctcgtccccc tttttgctgg acggtaggga
    11041 tggggattct cgggacccct cctcttcctc ttcaaggtca ccagacagag atgctactgg
    11101 ggcaacggaa gaaaagctgg gtgcggcctg tgaggatcag cttatcgatg ataagctgtc
    11161 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
    11221 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
    11281 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    11341 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    11401 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    11461 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    11521 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    11581 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
    11641 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    11701 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    11761 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    11821 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    11881 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
    11941 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    12001 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    12061 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    12121 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    12181 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    12241 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    12301 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    12361 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    12421 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    12481 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    12541 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
    12601 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12661 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12721 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12781 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12841 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12901 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12961 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    13021 ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc atctacctgc
    13081 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
    13141 atggggaagg ccatccagcc tcgcgtcgaa ttcgtcgacc tcgaaattct accgggtagg
    13201 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
    13261 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
    13321 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
    13381 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
    13441 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
    13501 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
    13561 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
    13621 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
    13681 tccgggcctt tcgacctgca tccatctaga tctcgagcag ctgaagctta ccatgaccga
    13741 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
    13801 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
    13861 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    13921 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
    13981 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
    14041 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
    14101 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
    14161 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
    14221 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
    14281 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
    14341 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tgatatcaga
    14401 tccccgggat gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag
    14461 tttttcctgt catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa
    14521 ggattggagc tacgggggtg ggggtggggt gggattagat aaatgcctgc tctttactga
    14581 aggctcttta ctattgcttt atgataatgt ttcatagttg gatatcataa tttaaacaag
    14641 caaaaccaaa ttaagggcca gctcattcct cccactcatg atctatagat ctatagatct
    14701 ctcgtgggat cattgttttt ctcttgattc ccactttgtg gttctaagta ctgtggtttc
    14761 caaatgtgtc agtttcatag cctgaagaac gagatcagca gcctctgttc cacatacact
    14821 tcattctcag tattgttttg ccaagttcta attccatcag aagctggtcg accaattctc
    14881 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
    14941 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
    15001 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
    15061 cataatatgt acatttatat tggctcatgt ccaatatgac cgccat
//
