LOCUS       sequence-452361-       15346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make INTACT lines under desired promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15346)
  TITLE     Siddiqi August 2024 Plasmids
REFERENCE   2  (bases 1 to 15346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pZP222_INTACT_AT
FEATURES             Location/Qualifiers
     source          1..15346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2245..3318
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3814..3836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3977..3996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4077..4094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4328..4347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5082..5873)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6394..6418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6496..6670
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6727..7260)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(7323..7999)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     8213..8235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8227..8244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8228..8244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9948..10910
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             10911..10940
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(11207..11458)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11483..11527)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(join(11558..12268,12269..12271))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(11558..12268,12269..12271))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(11600..11621)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     11949..11968
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12208..12229
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    12630..12754
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(12795..13100)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(12897..12916)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(13442..14101)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     14016..14035
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(14155..14161,14162..14179,14180..14185))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(14210..14334)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(14464..14510)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    14534..14553
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    14586..14605
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    14638..14657
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    14690..14709
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    14742..14761
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    14794..14813
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    14846..14865
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     complement(14919..14935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14919..14935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14932..14954)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14943..14959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14967..14973,14974..14991,14992..14997))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15012..15033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    15208..15232
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
     4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
     4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6061 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6121 gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc
     6181 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6241 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6301 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6481 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
     6541 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
     6601 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
     6661 tatggagaaa ctcgagcttg tcgatcgact ctaggccggg aagccgatct cggcttgaac
     6721 gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc
     6781 caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac
     6841 ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc
     6901 ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg
     6961 ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc
     7021 agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg
     7081 atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg
     7141 catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc
     7201 acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaccat
     7261 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     7321 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     7381 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     7441 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     7501 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     7561 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     7621 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     7681 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     7741 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     7801 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     7861 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     7921 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     7981 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     8041 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagca ttcgccattc
     8101 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     8161 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     8221 cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcac tggattttgg
     8281 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
     8341 gtttcttata tgctcaacac atgagcgaaa ccctataaga accctaattc ccttatctgg
     8401 gaactactca cacattattc tggagaaaaa tagagagaga tagatttgta gagagagact
     8461 ggtgattttt gcggactcta gaggatcccc gggtaccgac aaaatttaga acgaacttaa
     8521 ttatgatctc aaatacattg atacatatct catctagatc taggttatca ttatgtaaga
     8581 aagttttgac gaatatggca cgacaaaatg gctagactcg atgtaattgg tatctcaact
     8641 caacattata cttataccaa acattagtta gacaaaattt aaacaactat tttttatgta
     8701 tgcaagagtc agcatatgta taattgattc agaatcgttt tgacgagttc ggatgtagta
     8761 gtagccatta tttaatgtac atactaatcg tgaatagtga atatgatgaa acattgtatc
     8821 ttattgtata aatatccata aacacatcat gaaagacact ttctttcacg gtctgaatta
     8881 attatgatac aattctaata gaaaacgaat taaattacgt tgaattgtat gaaatctaat
     8941 tgaacaagcc aaccacgacg acgactaacg ttgcctggat tgactcggtt taagttaacc
     9001 actaaaaaaa cggagctgtc atgtaacacg cggatcgagc aggtcacagt catgaagcca
     9061 tcaaagcaaa agaactaatc caagggctga gatgattaat tagtttaaaa attagttaac
     9121 acgagggaaa aggctgtctg acagccaggt cacgttatct ttacctgtgg tcgaaatgat
     9181 tcgtgtctgt cgattttaat tatttttttg aaaggccgaa aataaagttg taagagataa
     9241 acccgcctat ataaattcat atattttcct ctccgctttg aattgtctcg ttgtcctcct
     9301 cactttcatc agccgttttg aatctccggc gacttgacag agaagaacaa ggaagaagac
     9361 taagagagaa agtaagagat aatccaggag attcattctc cgttttgaat cttcctcaat
     9421 ctcatcttct tccgctcttt ctttccaagg taataggaac tttctggatc tactttattt
     9481 gctggatctc gatcttgttt tctcaatttc cttgagatct ggaattcgtt taatttggat
     9541 ctgtgaacct ccactaaatc ttttggtttt actagaatcg atctaagttg accgatcagt
     9601 tagctcgatt atagctacca gaatttggct tgaccttgat ggagagatcc atgttcatgt
     9661 tacctgggaa atgatttgta tatgtgaatt gaaatctgaa ctgttgaagt tagattgaat
     9721 ctgaacactg tcaatgttag attgaatctg aacactgttt aaggttagat gaagtttgtg
     9781 tatagattct tcgaaacttt aggatttgta gtgtcgtacg ttgaacagaa agctatttct
     9841 gattcaatca gggtttattt gactgtattg aactcttttt gtgtgtttgc agctcataaa
     9901 aaatggctga ggctgatgat attcaaccaa tcgtgtgtga cctcgagatg aaggataaca
     9961 ccgtgccatt gaaactcatt gctcttctcg ctaatggaga gttccactct ggagaacaac
    10021 ttggagagac actcggaatg tctagagctg ctatcaacaa acacatccaa acccttaggg
    10081 attggggagt ggatgttttc actgttcctg gaaaaggata ttctcttcct gagcctatcc
    10141 aacttctcaa cgctgaacaa atcctcggtc aactcgatgg tggttctgtg gctgtgctcc
    10201 ctgtgatcga ttctaccaac cagtatctcc tcgataggat cggagaattg aaatctggtg
    10261 atgcttgtgt ggctgagtat caacaggctg gtagaggaag gaggggtaga aaatggttta
    10321 gtcctttcgg agcaaacctc tatctctcaa tgttctggag gcttgagcaa ggacctgctg
    10381 ctgctattgg actttctctt gtgattggaa ttgtgatggc tgaagtgctt aggaaactcg
    10441 gagctgataa ggtgagggtg aaatggccaa acgatcttta tcttcaggat agaaaacttg
    10501 ctggaatctt ggtggagctt accggaaaaa ccggtgatgc tgctcaaatc gtgatcggtg
    10561 ctggtatcaa catggctatg aggagagtgg aggagtctgt tgtgaatcag ggatggatca
    10621 ctttacaaga ggctggtatc aatctcgata gaaacacact cgctgctatg ctcattagag
    10681 agcttagggc tgctttagaa ctttttgaac aggagggatt agcaccatat cttagtagat
    10741 gggagaaact cgataacttc atcaataggc ctgtgaaact catcatcgga gataaggaga
    10801 ttttcggaat ctctagggga atcgataagc aaggagcttt gttactcgag caggatggaa
    10861 tcatcaaacc ttggatgggt ggagagattt cattgaggag tgctgagaag gaacaaaaac
    10921 tcatctctga ggaggatctt ggaggaaaac tcatctctga ggaggatctc ggtggaaaac
    10981 tcatcagtga ggaggatctc tgatacgtat ctccataata atgtgtgagt agttcccaga
    11041 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
    11101 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
    11161 atccagtact aaaatccaga tccccgtcga ccgatatccg aattccatct agtaacatag
    11221 atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
    11281 attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc
    11341 attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg
    11401 caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatcat
    11461 cgattcaaga tccaccagta tcctcatgcc attcaatctt ctgagcttcg aagatatcgt
    11521 taagtccacc agccatggca gcggccgcag ctccggactt gtacagctcg tccatgccga
    11581 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
    11641 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
    11701 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
    11761 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
    11821 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
    11881 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
    11941 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
    12001 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
    12061 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
    12121 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
    12181 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
    12241 ccccggtgaa cagctcctcg cccttgctca cagcaattgc ctcaacctcg gattcttcct
    12301 gtggaccacg tttgatgaca tcaagcatga gcttgctgga ttctttagca tagacgtgaa
    12361 cagcagaagt gccatcacca tcaggctcgt tctggaagtg tttgttggca gtagcaaagg
    12421 ccaaatcttc gatgcgcttg gcgtcttgct cagcctcttc aacagacaaa agaccgtact
    12481 tcctggagaa gatggaaggg gtggtaatgt tcttggtcat ccgctcaaca agcatgagac
    12541 gggtactctt actcggtggc cacatcttca ctgacaaaac acggttctgt gtggttttcg
    12601 ctgaatgatt catggtaccg gcctcgagca ccactttgta caagaaagct gaacgagaaa
    12661 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
    12721 aatactgtaa aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata
    12781 agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
    12841 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata
    12901 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
    12961 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc
    13021 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
    13081 gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat
    13141 ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc
    13201 acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc
    13261 atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc
    13321 atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc
    13381 aaaaatcagc gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatccacgcg
    13441 tttacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
    13501 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
    13561 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
    13621 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
    13681 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
    13741 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
    13801 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
    13861 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
    13921 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
    13981 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
    14041 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
    14101 ttttagcttc cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat
    14161 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg
    14221 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
    14281 attgatattt atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtggcgcg
    14341 ggccccccct cgaggtcgac aagcttgcta gctgtagttg tagaatgtaa aatgtaatgt
    14401 tgttgttgtt tgttgttgtt gttggtaatt gttgtaaaaa tacgcgcgtc tagcttcagc
    14461 gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaagatcgat
    14521 ccactagtct ttcaattgtg agcgctcaca attctttctc ttccctttct tctttctagt
    14581 ctttcaattg tgagcgctca caattctttc tcttcccttt cttctttcta gtctttcaat
    14641 tgtgagcgct cacaattctt tctcttccct ttcttctttc tagtctttca attgtgagcg
    14701 ctcacaattc tttctcttcc ctttcttctt tctagtcttt caattgtgag cgctcacaat
    14761 tctttctctt ccctttcttc tttctagtct ttcaattgtg agcgctcaca attctttctc
    14821 ttccctttct tctttctagt ctttcaattg tgagcgctca caattctttc tcttcccttt
    14881 cttctttcta gatttcttga gctcgaattc gtaatcatgt catagctgtt tcctgtgtga
    14941 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    15001 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    15061 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    15121 ggtttgcgta ttggctagag cgctctagag cagcttgagc ttggatcaga ttgtcgtttc
    15181 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
    15241 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
    15301 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaa
//
