LOCUS       sequence-452373-        4905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            mBaojin with URA3 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4905)
  TITLE     Teusink Lab yeast expression plasmids
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-ymBaojin-URA3
FEATURES             Location/Qualifiers
     source          1..4905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(568..672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     740..758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(796..818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     918..937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1018..1036
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1018..1035
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1106..1807
                     /codon_start=1
                     /product="bright and photostable monomeric green 
                     fluorescent protein derived from StayGold (Zhang et al., 
                     2024)"
                     /label=mBaoJin
                     /label=mStayGold
                     /note="/vntifkey=21"
                     /translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
                     LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
                     EAHLKGMDELYK"
     terminator      1848..2035
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1903..1927)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2110..2453
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3272..3469
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3576..3595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3577..3595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3682..3699)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3853..4441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3933..3952)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4612..4905,1..498,499..567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
       61 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      961 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     1021 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     1081 cggtgacggt gctggtttaa ttaacatggt ttctaagggt gaagaagaaa acatggcttc
     1141 tactccattc aagttccaat tgaagggtac tattaacggt aagtctttca ctgttgaagg
     1201 tgaaggtgaa ggtaactctc atgaaggttc tcacaagggt aagtacgttt gtacttctgg
     1261 taagttgcca atgtcttggg ctgctttggg tactactttc ggttacggta tgaagtacta
     1321 cactaagtac ccatctggtt taaagaactg gttcagagaa gttatgccag gtggtttcac
     1381 ttacgataga catattcaat acaagggtga tggttctatt catgctaagc atcaacattt
     1441 catgaagaac ggtacttacc ataacattgt tgaattcact ggtcaagatt tcaaggaaaa
     1501 ttctccagtt ttgaccggtg atatgaacgt ttctttgcca aatgaagttc cacaaattcc
     1561 aagagatgat ggtgttgaat gtccagttac tttattgtac ccattgttgt ctgataagtc
     1621 taagtatgtt gaagctcatc aatacactat ttgtaagcca ttgcataacc aaccagctcc
     1681 agatgtccca taccattgga ttagaaagca atacactcaa tctaaggatg atgctgaaga
     1741 aagagatcat atttgtcaat ctgaaacttt agaagctcat ttgaagggta tggatgaatt
     1801 gtacaagtaa gcggccgcct cgaggtcggc gcgccacttc taaataagcg aatttcttat
     1861 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     1921 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     1981 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat
     2041 ccgctaggga taacagggta atatagatct gtttagcttg cctcgtcccc gccgggtcac
     2101 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
     2161 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
     2221 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcgg
     2281 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
     2341 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
     2401 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgacag
     2461 tcaacactaa gacctatagt gagagagcag aaactcatgc ctcaccagta gcacaacgat
     2521 tatttcgatt aatggaactg aagaaaacca atttatgtgc atcaattgat gttgatacca
     2581 ctaaggaatt ccttgaatta attgataaat tgggtcctta tgtatgctta atcaagactc
     2641 atattgatat aatcaatgat ttttcctatg aatccactat tgaaccatta ttagaacttt
     2701 cacgtaaaca tcaatttatg atttttgaag atagaaaatt tgctgatatt ggtaataccg
     2761 tgaagaaaca atatattggt ggagtttata aaattagtag ttgggcagat attactaatg
     2821 ctcatggtgt cactgggaat ggagtagttg aaggattaaa acagggagct aaagaaacca
     2881 ccaccaacca agagccaaga gggttattga tgttagctga attatcatca gtgggatcat
     2941 tagcatatgg agaatattct caaaaaactg ttgaaattgc taaatccgat aaggaatttg
     3001 ttattggatt tattgcccaa cgtgatatgg gtggacaaga agaaggattt gattggctta
     3061 ttatgacacc tggagttgga ttagatgata aaggtgatgg attaggacaa caatatagaa
     3121 ctgttgatga agttgttagc actggaactg atattatcat tgttggtaga ggattgtttg
     3181 gtaaaggaag agatccagat attgaaggta aaaggtatag agatgctggt tggaatgctt
     3241 atttgaaaaa gactggccaa ttataatcag tactgacaat aaaaagattc ttgttttcaa
     3301 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
     3361 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     3421 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
     3481 atactaacgc cgccatccag tgtcgaaaac gagctcgaat tcatcgatga tatcagatcc
     3541 actagtggcc tatgcggccg cggatctgcc ggtctcccta tagtgagtcg tattaatttc
     3601 gataagccag gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3661 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3721 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3781 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3841 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3901 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3961 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4021 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4081 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4141 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4201 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4261 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4321 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4381 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4441 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4501 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4561 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4621 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4681 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4741 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4801 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4861 ttgttgccgg gaagctagag taagtagttc gccagttaat agttt
//
