LOCUS       sequence-452374-        5133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            emiRFP670 with URA3 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5133)
  TITLE     Teusink Lab yeast expression plasmids
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-yemiRFP670-URA3
FEATURES             Location/Qualifiers
     source          1..5133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(568..672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     740..758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(796..818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     918..937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1018..1036
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1018..1035
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      2076..2263
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2131..2155)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2338..2681
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3500..3697
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3804..3823)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3805..3823)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3910..3927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4081..4669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4161..4180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4840..5133,1..498,499..567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
       61 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      961 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     1021 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     1081 cggtgacggt gctggtttaa ttaacatggc tgaaggttct gttgctagac aaccagattt
     1141 gttgacttgt gaacatgaag aaattcattt ggctggttct attcaaccac atggtgcttt
     1201 gttggtcgtt tctgaacatg accatagagt tattcaagcc tctgctaatg ccgctgaatt
     1261 cttaaacttg ggttctgttt taggtgttcc attagctgaa attgatggtg atttgttaat
     1321 taagatttta ccacatttag atccaaccgc tgaaggtatg ccagttgctg ttagatgtag
     1381 aatcggtaat ccttccactg aatactgtgg tttgatgcat agaccaccag aaggtggttt
     1441 gattattgaa ttggaaagag ctggtccatc tattgattta tccggtactt tggctccagc
     1501 tttggaaaga attagaactg ctggttcttt aagagctttg tgtgatgaca ctgttttgtt
     1561 attccaacaa tgtactggtt atgatagagt tatggtttac agattcgatg aacaaggtca
     1621 tggtttagtt ttctctgaat gtcatgtccc tggtttggaa tcttacttcg gtaatagata
     1681 cccatcctct actgttccac aaatggctag acaattatac gttagacaaa gagttagagt
     1741 tttggtcgat gttacttacc aaccagttcc attggaacca agattatccc cattgactgg
     1801 tagagattta gatatgtctg gttgtttctt aagatctatg tctccttgtc atttgcaatt
     1861 cttgaaggat atgggtgtta gagctacttt ggccgtttct ttagttgttg gtggtaagtt
     1921 gtggggtttg gttgtttgtc accattactt gccaagattc attagattcg aattaagagc
     1981 tatctgtaag agattggctg aaagaattgc tactagaatt accgctttag aatcttaagc
     2041 ggccgcctcg aggtcggcgc gccacttcta aataagcgaa tttcttatga tttatgattt
     2101 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     2161 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     2221 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
     2281 acagggtaat atagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
     2341 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
     2401 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
     2461 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcggac ctgcgagcag
     2521 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
     2581 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg
     2641 ctaggataca gttctcacat cacatccgaa cataaacaac catgacagtc aacactaaga
     2701 cctatagtga gagagcagaa actcatgcct caccagtagc acaacgatta tttcgattaa
     2761 tggaactgaa gaaaaccaat ttatgtgcat caattgatgt tgataccact aaggaattcc
     2821 ttgaattaat tgataaattg ggtccttatg tatgcttaat caagactcat attgatataa
     2881 tcaatgattt ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc
     2941 aatttatgat ttttgaagat agaaaatttg ctgatattgg taataccgtg aagaaacaat
     3001 atattggtgg agtttataaa attagtagtt gggcagatat tactaatgct catggtgtca
     3061 ctgggaatgg agtagttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag
     3121 agccaagagg gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag
     3181 aatattctca aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta
     3241 ttgcccaacg tgatatgggt ggacaagaag aaggatttga ttggcttatt atgacacctg
     3301 gagttggatt agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag
     3361 ttgttagcac tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag
     3421 atccagatat tgaaggtaaa aggtatagag atgctggttg gaatgcttat ttgaaaaaga
     3481 ctggccaatt ataatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
     3541 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     3601 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     3661 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg
     3721 ccatccagtg tcgaaaacga gctcgaattc atcgatgata tcagatccac tagtggccta
     3781 tgcggccgcg gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt
     3841 taacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3901 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3961 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4261 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4501 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4561 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4621 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4681 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4741 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4801 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4861 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4921 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4981 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5041 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5101 agctagagta agtagttcgc cagttaatag ttt
//
