LOCUS       sequence-452389-       10060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of human PLAG1 with 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Chen KS, Stroup EK, Budhipramono A, Rakheja D, Nichols-Vinueza D, Xu
            L, Stuart SH, Shukla AA, Fraire C, Mendell JT, Amatruda JF
  TITLE     Mutations in microRNA processing genes in Wilms tumors derepress the
            IGF2 regulator PLAG1.
  JOURNAL   Genes Dev. 2018 Aug 1;32(15-16):996-1007. doi: 
            10.1101/gad.313783.118. Epub 2018 Jul 19.
  PUBMED    30026293
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2018-08-1"; volume: "32(15-16):996-1007. doi"; pages: 
            " 10.1101/gad.313783.118. Epub 2018 Jul 19"
COMMENT     Sequence Label: pLXC2B-PLAG1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             340..405
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(409..433)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    473..1061
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(526..546)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(944..955)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1133..1366
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1444..1565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1481..1500)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1535..1554
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1605..1740
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1760..1779)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1761..1779)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1789..1806)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1789..1805)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1798..1820)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1947..2402
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2034..2053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2244..2265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2428..2532
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2533..2601,2602..3393)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2751..2770)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3564..4152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4053..4072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4306..4323
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4440..4461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4476..4481,4482..4499,4500..4506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4514..4530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4519..4541
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4538..4554
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4538..4554
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4573..4593
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4575..4593
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4621..4847
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4848..5028
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5075..5200
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5693..5926
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6111..6155
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6330..6830
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6749..6767
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(6839..6841,6842..6844,6845..7555)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6884..6905)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7145..7164)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7492..7513
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7565..7618
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7634..8029
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8089..8206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8323..8626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8627..8830
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8781..8801
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    8853..8877
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attgggtccc tagatgatgg tgcaggagac ctctccctat ccaaaagctc tatctccatc
       61 agtgaccccc taaacacacc agcattggat ttttctcagt tgtttaattt cataccttta
      121 aatggtcctc cctataatcc tctatcagtg gggagccttg gaatgagcta ttcccaggaa
      181 gaagcacatt cttctgtttc ccagctcccc ccacaaacac aggatcttca agatcctgca
      241 aacactatag ggcttgggtc tctgcactca ctgtcagcag ctttcaccag cagtttaagc
      301 acaagtacca ccctcccacg tttccatcaa gcttttcagg actacaagga ccacgacggt
      361 gactacaagg accacgacat cgactacaag gacgacgacg acaagtgaac ccagctttct
      421 tgtacaaagt ggtttaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
      481 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
      541 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
      601 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
      661 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
      721 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
      781 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
      841 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
      901 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
      961 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     1021 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     1081 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     1141 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     1201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     1381 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     1441 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1561 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     1621 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     1681 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     1741 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     1801 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     1861 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     1921 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     1981 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2041 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2101 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2161 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2221 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2281 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2341 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     2401 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2461 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2521 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2581 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2641 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2701 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2761 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2821 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2881 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2941 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3001 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3061 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3121 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3181 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3241 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3301 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3361 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3421 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3481 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3541 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3601 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3661 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagta
     3721 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3781 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3841 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3901 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3961 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4021 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4081 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4141 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4201 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4261 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4321 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4381 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4441 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     4501 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     4561 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     4621 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     4681 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     4741 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     4801 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     4861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     5041 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     5101 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     5161 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     5221 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     5281 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     5341 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     5401 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     5461 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     5521 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     5581 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     5641 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     5701 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     5761 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     5821 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     5881 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     5941 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     6001 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     6061 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     6121 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     6181 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     6241 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     6301 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
     6361 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     6421 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     6481 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     6541 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     6601 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     6661 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     6721 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     6781 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccat
     6841 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     6901 cgacgtaaac ggccacaggt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     6961 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     7021 cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     7081 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgta ccatcttctt
     7141 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     7201 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     7261 gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
     7321 catcaaggcc cacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     7381 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     7441 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     7501 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggctc
     7561 cggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccact
     7621 cgagggtacc accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     7681 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     7741 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     7801 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     7861 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     7921 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     7981 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcc tgcagctgca
     8041 gtagtaagaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     8101 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     8161 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     8221 ggacagcaga gatccacttt ggcgccggct cgagggggcc cggggttatt aatagtaatc
     8281 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     8341 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     8401 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     8461 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     8521 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     8581 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     8641 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     8701 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     8761 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     8821 aagcagagct ctctggctaa ctgtcgggat caacaagttt gtacaaaaaa gcaggctaga
     8881 aggcctggtt taggctgcga tggccactgt cattcctggt gatttgtcag aagtaagaga
     8941 tacccagaaa gtcccttcag ggaaacgtaa gcgtggtgaa accaaaccaa gaaaaaactt
     9001 tccttgccaa ctgtgtgaca aggcctttaa cagtgttgag aaattaaagg ttcactccta
     9061 ctctcacaca ggagagaggc cctacaagtg catacaacaa gactgcacca aggcctttgt
     9121 ttctaagtac aaattacaaa ggcacatggc tactcattct cctgagaaaa cccacaagtg
     9181 taattattgt gagaaaatgt ttcaccggaa agatcatctg aagaatcacc tccatacaca
     9241 cgaccctaac aaagagacgt ttaagtgcga agaatgtggc aagaactaca ataccaagct
     9301 tggatttaaa cgtcacttgg ccttgcatgc cgcaacaagt ggtgacctca cctgtaaggt
     9361 atgtttgcaa acttttgaaa gcacgggagt gcttctggag caccttaaat ctcatgcagg
     9421 caagtcgtct ggtggggtta aagaaaaaaa gcaccagtgc gaacattgtg atcgccggtt
     9481 ctacacccga aaggatgtcc ggagacacat ggtggtgcac actggaagaa aggacttcct
     9541 ctgtcagtat tgtgcacaga gatttgggcg aaaggatcac ctgactcgac atatgaagaa
     9601 gagtcacaat caagagcttc tgaaggtcaa aacagaacca gtggatttcc ttgacccatt
     9661 tacctgcaat gtgtctgtgc ctataaaaga cgagctcctt ccggtgatgt ccttaccttc
     9721 cagtgaactg ttatcaaagc cattcacaaa cactttgcag ttaaacctct acaacactcc
     9781 atttcagtcc atgcagagct cgggatctgc ccaccaaatg atcacaactt tacctttggg
     9841 aatgacatgc ccaatagata tggacactgt tcatccctct caccaccttt ctttcaaata
     9901 tccgttcagt tctacctcat atgcaatttc tattcctgaa aaagaacagc cattaaaggg
     9961 ggaaattgag agttacctga tggagttaca aggtggcgtg ccctcttcat cccaagattc
    10021 tcaagcatcg tcatcatcta agctagggtt ggatcctcag
//
