LOCUS       sequence-452414-        5259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            emiRFP670 with Kan selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5259)
  TITLE     Teusink Lab yeast expression plasmids
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-yemiRFP670-Kan
FEATURES             Location/Qualifiers
     source          1..5259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     125..143
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(181..203)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     303..322
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        403..421
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     403..420
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1593..1780
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1648..1672)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            1855..3211
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1855..2198
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2199..3008
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2266..2285)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      3014..3211
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3315..3334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3316..3334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3421..3438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3592..4180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3672..3691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4351..5142,5143..5211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4974..4993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(5212..5259,1..57))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
       61 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
      121 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
      181 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      241 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      301 tgcggcatca gagcagattg tactgagagt gcaccatatg gacatattgt cgttagaacg
      361 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
      421 aacgcggccg ccagctgaag cttcgtacgc tgcaggtcga cggatcggtg acggtgctgg
      481 tttaattaac atggctgaag gttctgttgc tagacaacca gatttgttga cttgtgaaca
      541 tgaagaaatt catttggctg gttctattca accacatggt gctttgttgg tcgtttctga
      601 acatgaccat agagttattc aagcctctgc taatgccgct gaattcttaa acttgggttc
      661 tgttttaggt gttccattag ctgaaattga tggtgatttg ttaattaaga ttttaccaca
      721 tttagatcca accgctgaag gtatgccagt tgctgttaga tgtagaatcg gtaatccttc
      781 cactgaatac tgtggtttga tgcatagacc accagaaggt ggtttgatta ttgaattgga
      841 aagagctggt ccatctattg atttatccgg tactttggct ccagctttgg aaagaattag
      901 aactgctggt tctttaagag ctttgtgtga tgacactgtt ttgttattcc aacaatgtac
      961 tggttatgat agagttatgg tttacagatt cgatgaacaa ggtcatggtt tagttttctc
     1021 tgaatgtcat gtccctggtt tggaatctta cttcggtaat agatacccat cctctactgt
     1081 tccacaaatg gctagacaat tatacgttag acaaagagtt agagttttgg tcgatgttac
     1141 ttaccaacca gttccattgg aaccaagatt atccccattg actggtagag atttagatat
     1201 gtctggttgt ttcttaagat ctatgtctcc ttgtcatttg caattcttga aggatatggg
     1261 tgttagagct actttggccg tttctttagt tgttggtggt aagttgtggg gtttggttgt
     1321 ttgtcaccat tacttgccaa gattcattag attcgaatta agagctatct gtaagagatt
     1381 ggctgaaaga attgctacta gaattaccgc tttagaatct taagcggccg cctcgagcca
     1441 cgcgccggag ccgccggcca cgttgatggc cgagtagccg cggccggtca tgaactcggc
     1501 ggcgcgggcg gagcggccgc cggtgcggca gatcaggtag tagtccgtgt ccgggtccag
     1561 ctcctcgagg tcggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta
     1621 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     1681 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     1741 tatagtatga ggtcgctctt attgaccaca cctctaccgg cagatccgct agggataaca
     1801 gggtaatata gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
     1861 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
     1921 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
     1981 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
     2041 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
     2101 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
     2161 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg
     2221 tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     2281 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
     2341 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     2401 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     2461 ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat
     2521 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     2581 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     2641 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     2701 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
     2761 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     2821 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     2881 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     2941 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     3001 ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata
     3061 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt
     3121 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt
     3181 caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc
     3241 agtttaaacg agctcgaatt catcgatgat atcagatcca ctagtggcct atgcggccgc
     3301 ggatctgccg gtctccctat agtgagtcgt attaatttcg ataagccagg ttaacctgca
     3361 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3421 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3481 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3541 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3601 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3661 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3721 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3781 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3841 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3901 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3961 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4021 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4081 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4141 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4201 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4261 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4321 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4381 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4441 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4501 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4561 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4621 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4681 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4741 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4801 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4861 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4921 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4981 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5041 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5101 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5161 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5221 ttttcaatat tattgaagca tttatcaggg ttattgtct
//
