LOCUS       sequence-452430-       13318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate level 1, position 2, PE2max-NC expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   Vu TV, Nguyen NT, Kim J, Song YJ, Nguyen TH, Kim JY
  TITLE     Optimized dicot prime editing enables heritable desired edits in 
            tomato and Arabidopsis.
  JOURNAL   Nat Plants. 2024 Sep 6. doi: 10.1038/s41477-024-01786-w.
  PUBMED    39242983
REFERENCE   2  (bases 1 to 13318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2024 Sep 6. doi: 10.1038/s41477-024-01786-w."
COMMENT     Sequence Label: pICH47742::p35S-PE2max-NC::tEURb7
FEATURES             Location/Qualifiers
     source          1..13318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1023..1368
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1311..1331
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1495..1515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1516..5616
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5674..5694
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7795..7815
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7825..7851
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_feature    9021..9045
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9201..10349)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(10685..11273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10765..10784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11444..12235,12236..12304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12067..12086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12305..12409)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12496..13127
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    13219..13243
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggaattcc aatcccacaa aaatctgagc
       61 ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact
      121 actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg
      181 cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa
      241 gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca
      301 aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc
      361 aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag
      421 agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa
      481 ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc
      541 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc
      601 tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg
      661 ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat
      721 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
      781 ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc
      841 atggagtcta agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag
      901 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
      961 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     1021 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     1081 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     1141 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     1201 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     1261 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     1321 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata
     1381 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     1441 tttacaatta tcgatacaat gaaacggaca gccgacggaa gcgagttcga gtcaccaaag
     1501 aagaagcgga aagtcgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg
     1561 ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc
     1621 aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc
     1681 gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag
     1741 aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc
     1801 ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac
     1861 cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac
     1921 cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg
     1981 gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc
     2041 gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc
     2101 gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg
     2161 agcaagagca gaaagctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc
     2221 ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc
     2281 gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac
     2341 aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg
     2401 tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc
     2461 ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa
     2521 gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag
     2581 aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc
     2641 aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaagagagag
     2701 gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg
     2761 ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac
     2821 cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc
     2881 aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg
     2941 aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc
     3001 aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag
     3061 tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag
     3121 cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac
     3181 cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac
     3241 tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat
     3301 ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg
     3361 gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg
     3421 aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac
     3481 accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc
     3541 aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg
     3601 atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag
     3661 ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc
     3721 atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc
     3781 gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac
     3841 agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg
     3901 aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg
     3961 cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac
     4021 gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg
     4081 ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg
     4141 aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag
     4201 ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc
     4261 atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac
     4321 tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc
     4381 accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc
     4441 gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc
     4501 ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac
     4561 gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac
     4621 ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag
     4681 atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag
     4741 ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa
     4801 aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc
     4861 gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc
     4921 cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa
     4981 ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag
     5041 aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc
     5101 aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct
     5161 gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg
     5221 tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag
     5281 ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc
     5341 tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag
     5401 caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc
     5461 aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac
     5521 accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac
     5581 gagacacgga tcgacctgtc tcagctggga ggtgactccg gcggaagctc tggtggcagc
     5641 aagcggaccg ccgacggctc tgaattcgag agccctaaga agaaaagaaa ggtgagcgga
     5701 ggctctagcg gcggaagcac cctgaacatt gaagatgagt atagactgca tgaaacaagc
     5761 aaggaacccg acgtgtccct gggctccacc tggctgtccg actttcccca ggcctgggcc
     5821 gagacaggag gaatgggcct ggccgtgcgg caggcacccc tgatcatccc tctgaaggcc
     5881 acctctacac ccgtgagcat caagcagtac cctatgtctc aggaggccag actgggcatc
     5941 aagcctcaca tccagaggct gctggaccag ggcatcctgg tgccatgcca gagcccctgg
     6001 aacacaccac tgctgcccgt gaagaagcca ggcaccaatg actatagacc cgtgcaggat
     6061 ctgagagagg tgaacaagag ggtggaggat atccacccca ccgtgcccaa cccttacaat
     6121 ctgctgtccg gcctgccccc ttctcaccag tggtatacag tgctggacct gaaggatgcc
     6181 ttcttttgtc tgagactgca ccctaccagc cagccactgt tcgcctttga gtggagggac
     6241 cctgagatgg gcatctctgg ccagctgacc tggacacgcc tgcctcaggg cttcaagaat
     6301 agcccaacac tgtttaacga ggccctgcac cgcgacctgg cagatttccg gatccagcac
     6361 ccagatctga tcctgctgca gtacgtggac gatctgctgc tggccgccac cagcgagctg
     6421 gattgccagc agggaacacg cgccctgctg cagaccctgg gaaacctggg atatagggca
     6481 tccgccaaga aggcccagat ctgtcagaag caggtgaagt acctgggcta tctgctgaag
     6541 gagggccaga gatggctgac agaggccagg aaggagacag tgatgggcca gccaacaccc
     6601 aagaccccaa gacagctgag ggagttcctg ggcaaagcag gattttgcag gctgttcatc
     6661 ccaggattcg cagagatggc agcacctctg tacccactga ccaagccggg caccctgttt
     6721 aattggggcc ctgaccagca gaaggcctat caggagatca agcaggccct gctgacagca
     6781 ccagccctgg gcctgccaga cctgaccaag cctttcgagc tgtttgtgga tgagaagcag
     6841 ggctacgcca agggcgtgct gacccagaag ctgggaccat ggagacggcc cgtggcctat
     6901 ctgtccaaga agctggaccc agtggcagca ggatggccac catgcctgag gatggtggca
     6961 gcaatcgccg tgctgacaaa ggatgccggc aagctgacca tgggacagcc actggtcatc
     7021 ctggcaccac acgcagtgga ggccctggtg aagcagcctc cagatcgctg gctgtctaac
     7081 gcccggatga cacactacca ggccctgctg ctggacaccg atcgcgtgca gtttggccct
     7141 gtggtggccc tgaatccagc caccctgctg cctctgccag aggagggcct gcagcacaac
     7201 tgtctggaca tcctggcaga ggcacacgga acaaggccag acctgaccga tcagcccctg
     7261 cctgacgccg atcacacatg gtataccgat ggaagctccc tgctgcagga gggccagagg
     7321 aaggcaggag cagcagtgac cacagagaca gaagtgatct gggccaaggc cctgccagca
     7381 ggcacatccg cccagcgggc cgagctgatc gccctgaccc aggccctgaa gatggccgag
     7441 ggcaagaagc tgaacgtgta cacagactcc agatatgcct tcgccaccgc acacatccac
     7501 ggagagatct acaggcgccg gggctggctg acctctgagg gcaaggagat caagaacaag
     7561 gatgagatcc tggccctgct gaaggccctg tttctgccca agcggctgag catcatccac
     7621 tgtcctggac accagaaggg acactccgcc gaggcaaggg gcaatcggat ggccgaccag
     7681 gccgccagaa aggctgctat tactgaaact cccgacactt ccactctgct gattgaaaac
     7741 tcctcccctt ctggcggctc aaaaagaacc gccgacggca gcgaattcga gtctcccaag
     7801 aagaagagga aagtcggctc tggccctgcc gctaagagag tgaagctgga cgccacagtg
     7861 gtgtccggcc aaaagcagga ccgccagggc ggagaacgca gaaggtccca gctcgatagg
     7921 gatcagtgtg cctactgcaa ggagaagggc cactgggcca aagactgccc gaaaaagccg
     7981 cgcggcccac gcggcccaag gccacaaaca tccctccttt aagcttaaag cagaatgctg
     8041 agctaaaaga aaggcttttt ccattttcga gagacaatga gaaaagaaga agaagaagaa
     8101 gaagaagaag aagaagaaga agaaaagagt aaataataaa gccccacagg aggcgaagtt
     8161 cttgtagctc catgttatct aagttattga tattgtttgc cctatatttt atttctgtca
     8221 ttgtgtatgt tttgttcagt ttcgatctcc ttgcaaaatg cagagattat gagatgaata
     8281 aactaagtta tattattata cgtgttaata ttctcctcct ctctctagct agccttttgt
     8341 tttctctttt tcttatttga ttttctttaa atcaatccat tttaggagag ggccagggag
     8401 tgatccagca aaacatgaag attagaagaa acttccctct tttttttcct gaaaacaatt
     8461 taacgtcgag atttatctct ttttgtaatg gaatcatttc tacagttatg acaccaactc
     8521 ggtccatttg cacccctaat cataatagct ttaatatttc aagatattat taagttaacg
     8581 ttgtcaatat cctggaaatt ttgcaaaatg aatcaagcct atatggctgt aatatgaatt
     8641 taaaagcagc tcgatgtggt ggtaatatgt aatttacttg attctaaaaa aatatcccaa
     8701 gtattaataa tttctgctag gaagaaggtt agctacgatt tacagcaaag ccagaataca
     8761 aagaaccata aagtgattga agctcgaaat atacgaagga acaaatattt ttaaaaaaat
     8821 acgcaatgac ttggaacaaa agaaagtgat atattttttg ttcttaaaca agcatcccct
     8881 ctaaagaatg gcagttttcc tttgcatgta actattatgc tcccttcgtt acaaaaattt
     8941 tggactacta ttgggaactt cttctgaaaa tagtgcgcta ctattgtctt ctgcacgaag
     9001 tggtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     9061 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     9121 gtatgtgcat gccaaccaca gggttcccca gatcaggcgc tggctgctga acccccagcc
     9181 ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg
     9241 tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac
     9301 ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg
     9361 tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg
     9421 cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc
     9481 tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg
     9541 tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc
     9601 gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag
     9661 cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc
     9721 ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg
     9781 ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc
     9841 tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc
     9901 atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg
     9961 atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc
    10021 aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc
    10081 atcgacttcg ccaaacctgc cgcctcctgt tcaagacgac gcgaacgctc cacggcggcc
    10141 gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta
    10201 gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg
    10261 cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg
    10321 tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac
    10381 gccggggcaa tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa
    10441 tccaccttat cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg
    10501 gtattccgaa tcttgccctg cacgaatacc agcgacccct tgcccaaata cttgccgtgg
    10561 gcctcggcct gagagccaaa acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg
    10621 ccggcatcgt tgcgccacat ctaggatctg ccaggaaccg taaaaaggcc gcgttgctgg
    10681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    10981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    11041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    11101 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    11161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11221 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11281 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11341 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    11401 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    11461 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    11521 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    11581 cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    11641 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    11701 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    11761 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    11821 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    11881 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    11941 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    12001 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    12061 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    12121 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    12181 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    12241 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    12301 tcatactctt cctttttcaa tattattgaa gcatttatca aggttattgt ctcatgagcg
    12361 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acgaattggc
    12421 cagcgctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga
    12481 tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg
    12541 tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt
    12601 taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa
    12661 tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt
    12721 cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc
    12781 tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac
    12841 tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc
    12901 gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt
    12961 caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc
    13021 ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt gcagggccat
    13081 agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgca aaggagatcc
    13141 tgatctgact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
    13201 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
    13261 acttaataac acattgcgga cgtttttaat gtactggggt ggatgcagtg ggccccac
//
