LOCUS       sequence-452431-       12862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate level 1, position 2, PE2max-NC expression cassette for 
            Arabidopsis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12862)
  AUTHORS   Vu TV, Nguyen NT, Kim J, Song YJ, Nguyen TH, Kim JY
  TITLE     Optimized dicot prime editing enables heritable desired edits in 
            tomato and Arabidopsis.
  JOURNAL   Nat Plants. 2024 Sep 6. doi: 10.1038/s41477-024-01786-w.
  PUBMED    39242983
REFERENCE   2  (bases 1 to 12862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2024 Sep 6. doi: 10.1038/s41477-024-01786-w."
COMMENT     Sequence Label: pICH47742:: pRPS5a-PE2max-NC::tHSP
FEATURES             Location/Qualifiers
     source          1..12862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1735..1755
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1756..5856
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5914..5934
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8035..8055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8065..8091
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      8271..8519
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8565..8589
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(8745..9893)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(10229..10817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10309..10328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10988..11779,11780..11848))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11611..11630
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11849..11953)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12040..12671
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    12763..12787
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggatccct caacttttga ttcgctattt
       61 gcagtgcacc tgtggcgttc atcacatctt ttgtgacact gtttgcactg gtcattgcta
      121 ttacaaagga ccttcctgat gttgaaggag atcgaaagta agtaactgca cgcataacca
      181 ttttctttcc gctctttggc tcaatccatt tgacagtcaa agacaatgtt taaccagctc
      241 cgtttgatat attgtcttta tgtgtttgtt caagcatgtt tagttaatca tgcctttgat
      301 tgatcttgaa taggttccaa atatcaaccc tggcaacaaa acttggagtg agaaacattg
      361 cattcctcgg ttctggactt ctgctagtaa attatgtttc agccatatca ctagctttct
      421 acatgcctca ggtgaattca tctatttccg tcttaactat ttcggttaat taaagcacga
      481 acaccattac tgcatgtaga agcttgataa actatcgcca ccaatttatt tttgttgcga
      541 tattgttact ttcctcagta tgcagctttg aaaagaccaa ccctcttatc ctttaacaat
      601 gaacaggttt ttagaggtag cttgatgatt cctgcacatg tgatcttggc ttcaggctta
      661 attttccagg taaagcatta tgagatactc ttatatctct tacatacttt tgagataatg
      721 cacaagaact tcataactat atgctttagt ttctgcattt gacactgcca aattcattaa
      781 tctctaatat ctttgttgtt gatctttggt agacatgggt actagaaaaa gcaaactaca
      841 ccaaggtaaa atacttttgt acaaacataa actcgttatc acggaacatc aatggagtgt
      901 atatctaacg gagtgtagaa acatttgatt attgcaggaa gctatctcag gatattatcg
      961 gtttatatgg aatctcttct acgcagagta tctgttattc cccttcctct agctttcaat
     1021 ttcatggtga ggatatgcag ttttctttgt atatcattct tcttcttctt tgtagcttgg
     1081 agtcaaaatc ggttccttca tgtacataca tcaaggatat gtccttctga atttttatat
     1141 cttgcaataa aaatgcttgt accaattgaa acaccagctt tttgagttct atgatcactg
     1201 acttggttct aaccaaaaaa aaaaaatgtt taatttacat atctaaaagt aggtttaggg
     1261 aaacctaaac agtaaaatat ttgtatatta ttcgaatttc actcatcata aaaacttaaa
     1321 ttgcaccata aaattttgtt ttactattaa tgatgtaatt tgtgtaactt aagataaaaa
     1381 taatattccg taagttaacc ggctaaaacc acgtataaac cagggaacct gttaaaccgg
     1441 ttctttactg gataaagaaa tgaaagccca tgtagacagc tccattagag cccaaaccct
     1501 aaatttctca tctatataaa aggagtgaca ttagggtttt tgttcgtcct cttaaagctt
     1561 ctcgttttct ctgccgtctc tctcattcgc gcgacgcaaa cgatcttcag gtgatcttct
     1621 ttctccaaat cctctctcat aactctgatt tcgtacttgt gtatttgagc tcacgctctg
     1681 tttctctcac cacagccaat gaaacggaca gccgacggaa gcgagttcga gtcaccaaag
     1741 aagaagcgga aagtcgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg
     1801 ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc
     1861 aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc
     1921 gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag
     1981 aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc
     2041 ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac
     2101 cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac
     2161 cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg
     2221 gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc
     2281 gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc
     2341 gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg
     2401 agcaagagca gaaagctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc
     2461 ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc
     2521 gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac
     2581 aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg
     2641 tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc
     2701 ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa
     2761 gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag
     2821 aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc
     2881 aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaagagagag
     2941 gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg
     3001 ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac
     3061 cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc
     3121 aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg
     3181 aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc
     3241 aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag
     3301 tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag
     3361 cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac
     3421 cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac
     3481 tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat
     3541 ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg
     3601 gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg
     3661 aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac
     3721 accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc
     3781 aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg
     3841 atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag
     3901 ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc
     3961 atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc
     4021 gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac
     4081 agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg
     4141 aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg
     4201 cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac
     4261 gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg
     4321 ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg
     4381 aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag
     4441 ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc
     4501 atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac
     4561 tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc
     4621 accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc
     4681 gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc
     4741 ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac
     4801 gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac
     4861 ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag
     4921 atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag
     4981 ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa
     5041 aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc
     5101 gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc
     5161 cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa
     5221 ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag
     5281 aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc
     5341 aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct
     5401 gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg
     5461 tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag
     5521 ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc
     5581 tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag
     5641 caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc
     5701 aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac
     5761 accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac
     5821 gagacacgga tcgacctgtc tcagctggga ggtgactccg gcggaagctc tggtggcagc
     5881 aagcggaccg ccgacggctc tgaattcgag agccctaaga agaaaagaaa ggtgagcgga
     5941 ggctctagcg gcggaagcac cctgaacatt gaagatgagt atagactgca tgaaacaagc
     6001 aaggaacccg acgtgtccct gggctccacc tggctgtccg actttcccca ggcctgggcc
     6061 gagacaggag gaatgggcct ggccgtgcgg caggcacccc tgatcatccc tctgaaggcc
     6121 acctctacac ccgtgagcat caagcagtac cctatgtctc aggaggccag actgggcatc
     6181 aagcctcaca tccagaggct gctggaccag ggcatcctgg tgccatgcca gagcccctgg
     6241 aacacaccac tgctgcccgt gaagaagcca ggcaccaatg actatagacc cgtgcaggat
     6301 ctgagagagg tgaacaagag ggtggaggat atccacccca ccgtgcccaa cccttacaat
     6361 ctgctgtccg gcctgccccc ttctcaccag tggtatacag tgctggacct gaaggatgcc
     6421 ttcttttgtc tgagactgca ccctaccagc cagccactgt tcgcctttga gtggagggac
     6481 cctgagatgg gcatctctgg ccagctgacc tggacacgcc tgcctcaggg cttcaagaat
     6541 agcccaacac tgtttaacga ggccctgcac cgcgacctgg cagatttccg gatccagcac
     6601 ccagatctga tcctgctgca gtacgtggac gatctgctgc tggccgccac cagcgagctg
     6661 gattgccagc agggaacacg cgccctgctg cagaccctgg gaaacctggg atatagggca
     6721 tccgccaaga aggcccagat ctgtcagaag caggtgaagt acctgggcta tctgctgaag
     6781 gagggccaga gatggctgac agaggccagg aaggagacag tgatgggcca gccaacaccc
     6841 aagaccccaa gacagctgag ggagttcctg ggcaaagcag gattttgcag gctgttcatc
     6901 ccaggattcg cagagatggc agcacctctg tacccactga ccaagccggg caccctgttt
     6961 aattggggcc ctgaccagca gaaggcctat caggagatca agcaggccct gctgacagca
     7021 ccagccctgg gcctgccaga cctgaccaag cctttcgagc tgtttgtgga tgagaagcag
     7081 ggctacgcca agggcgtgct gacccagaag ctgggaccat ggagacggcc cgtggcctat
     7141 ctgtccaaga agctggaccc agtggcagca ggatggccac catgcctgag gatggtggca
     7201 gcaatcgccg tgctgacaaa ggatgccggc aagctgacca tgggacagcc actggtcatc
     7261 ctggcaccac acgcagtgga ggccctggtg aagcagcctc cagatcgctg gctgtctaac
     7321 gcccggatga cacactacca ggccctgctg ctggacaccg atcgcgtgca gtttggccct
     7381 gtggtggccc tgaatccagc caccctgctg cctctgccag aggagggcct gcagcacaac
     7441 tgtctggaca tcctggcaga ggcacacgga acaaggccag acctgaccga tcagcccctg
     7501 cctgacgccg atcacacatg gtataccgat ggaagctccc tgctgcagga gggccagagg
     7561 aaggcaggag cagcagtgac cacagagaca gaagtgatct gggccaaggc cctgccagca
     7621 ggcacatccg cccagcgggc cgagctgatc gccctgaccc aggccctgaa gatggccgag
     7681 ggcaagaagc tgaacgtgta cacagactcc agatatgcct tcgccaccgc acacatccac
     7741 ggagagatct acaggcgccg gggctggctg acctctgagg gcaaggagat caagaacaag
     7801 gatgagatcc tggccctgct gaaggccctg tttctgccca agcggctgag catcatccac
     7861 tgtcctggac accagaaggg acactccgcc gaggcaaggg gcaatcggat ggccgaccag
     7921 gccgccagaa aggctgctat tactgaaact cccgacactt ccactctgct gattgaaaac
     7981 tcctcccctt ctggcggctc aaaaagaacc gccgacggca gcgaattcga gtctcccaag
     8041 aagaagagga aagtcggctc tggccctgcc gctaagagag tgaagctgga cgccacagtg
     8101 gtgtccggcc aaaagcagga ccgccagggc ggagaacgca gaaggtccca gctcgatagg
     8161 gatcagtgtg cctactgcaa ggagaagggc cactgggcca aagactgccc gaaaaagccg
     8221 cgcggcccac gcggcccaag gccacaaaca tccctccttt aagcttggga tatgaagatg
     8281 aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc
     8341 ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatctca
     8401 ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct
     8461 gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt aaagataagc
     8521 gctactattg tcttctgcac gaagtggttt aaactatcag tgtttgacag gatatattgg
     8581 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
     8641 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc cccagatcag
     8701 gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc
     8761 accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag
     8821 gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg
     8881 cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc
     8941 cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg
     9001 ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg
     9061 ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg
     9121 tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg
     9181 taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg
     9241 atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg
     9301 tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg
     9361 aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg
     9421 gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg
     9481 aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc
     9541 ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc
     9601 atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcaaga
     9661 cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg
     9721 ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt
     9781 tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac
     9841 cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct
     9901 ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg
     9961 cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc
    10021 gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac
    10081 cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag
    10141 aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctagga tctgccagga
    10201 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10261 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10321 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10381 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10441 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10501 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10561 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10621 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    10681 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10741 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10801 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10861 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10921 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10981 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    11041 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    11101 ctggccccag tgctgcaatg ataccgcgag aaccacgctc accggctcca gatttatcag
    11161 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    11221 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    11281 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    11341 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    11401 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    11461 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    11521 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    11581 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    11641 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    11701 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11761 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11821 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11881 atcaaggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11941 taggggttcc gcgcacgaat tggccagcgc tgccattttt ggggtgaggc cgttcgcggc
    12001 cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag
    12061 aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa
    12121 aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga
    12181 aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg
    12241 tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc
    12301 ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc
    12361 ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg
    12421 ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc
    12481 cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt
    12541 tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg
    12601 tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc
    12661 cggtgagcgt cgcaaaggag atcctgatct gactgatggg ctgcctgtat cgagtggtga
    12721 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
    12781 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
    12841 gggtggatgc agtgggcccc ac
//
