LOCUS       sequence-452432-        4536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate level 0 acceptor vector for cloning altered epegRNA: 
            altered epegRNA being driven by U6 composite promoter (pU6cm) and 
            terminated by a dual terminator (EURb7)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Vu TV, Nguyen NT, Kim J, Song YJ, Nguyen TH, Kim JY
  TITLE     Optimized dicot prime editing enables heritable desired edits in 
            tomato and Arabidopsis.
  JOURNAL   Nat Plants. 2024 Sep 6. doi: 10.1038/s41477-024-01786-w.
  PUBMED    39242983
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2024 Sep 6. doi: 10.1038/s41477-024-01786-w."
COMMENT     Sequence Label: pICH41331::pU6cm-pegR::EURb7
FEATURES             Location/Qualifiers
     source          1..4536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     ncRNA           3492..3559
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaggagatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
     2281 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
     2341 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
     2401 cagaagatca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
     2461 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
     2521 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
     2581 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gatgttccaa
     2641 ccacatcttc aaagcaagtg gattgatgtg attggcagac atactgtccc acaaatgaag
     2701 atggaatctg taaaagaaaa cgcgtgaaat aatgcgtctg acaaaggtta ggtcggctgc
     2761 ctttaatcaa taccaaagtg gtccctacca cgatggaaaa actgtgcagt cggtttggct
     2821 ttttctgacg aacaaataag attcgtggcc gacaggtggg ggtccaccat gtgaaggcat
     2881 cttcagactc caataatgga gcaatgacgt aagggcttac gaaataagta agggtagttt
     2941 gggaaatgtc cactcacccg tcagtctata aatacttagc ccctccctca ttgttaaggg
     3001 agcaaaatct cagagagata gtcctagaga gagaaagaga gcaagtagcc tagaagtagt
     3061 caaggcggcg aagtattcag gcacgtggcc aggaagaaga aaagccaaga cgacgaaaac
     3121 aggtaagagc taagcatcta gaaagttgaa aacaatcttc aaaagtccca catcgcttag
     3181 ataagaaaac gaagctgagt ttatatacag ctagagtcga agtagtgatt gaacaaagca
     3241 ccagtggtct agtggtagaa tagtaccctg ccacggtaca gacccgggtt cgattcccgg
     3301 ctggtgcatt gtcttcaatt ccttacggat cgaagacaag tttaagagct atgctggaaa
     3361 cagcatagca agtttaaata aggctagtcc gttatcgact tgaaaaagtc gcaccgagtc
     3421 ggtgcttgtc ttctagacta gtcgacgaag acaacgcggt tctatctagt tacgcgttaa
     3481 accaactaga aggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct
     3541 tcggcatggc gaatgggact tttttttgct taaagcagaa tgctgagcta aaagaaaggc
     3601 tttttccatt ttcgagagac aatgagaaaa gaagaagaag aagaagaaga agaagaagaa
     3661 gaagaagaaa agagtaaata ataaagcccc acaggaggcg aagttcttgt agctccatgt
     3721 tatctaagtt attgatattg tttgccctat attttatttc tgtcattgtg tatgttttgt
     3781 tcagtttcga tctccttgca aaatgcagag attatgagat gaataaacta agttatatta
     3841 ttatacgtgt taatattctc ctcctctctc tagctagcct tttgttttct ctttttctta
     3901 tttgattttc tttaaatcaa tccattttag gagagggcca gggagtgatc cagcaaaaca
     3961 tgaagattag aagaaacttc cctctttttt ttcctgaaaa caatttaacg tcgagattta
     4021 tctctttttg taatggaatc atttctacag ttatgacacc aactcggtcc atttgcaccc
     4081 ctaatcataa tagctttaat atttcaagat attattaagt taacgttgtc aatatcctgg
     4141 aaattttgca aaatgaatca agcctatatg gctgtaatat gaatttaaaa gcagctcgat
     4201 gtggtggtaa tatgtaattt acttgattct aaaaaaatat cccaagtatt aataatttct
     4261 gctaggaaga aggttagcta cgatttacag caaagccaga atacaaagaa ccataaagtg
     4321 attgaagctc gaaatatacg aaggaacaaa tatttttaaa aaaatacgca atgacttgga
     4381 acaaaagaaa gtgatatatt ttttgttctt aaacaagcat cccctctaaa gaatggcagt
     4441 tttcctttgc atgtaactat tatgctccct tcgttacaaa aattttggac tactattggg
     4501 aacttcttct gaaaatagtg cgcttgagac cacgaa
//
