LOCUS       sequence-452433-       14885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reengineered geminiviral  replicon vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14885)
  AUTHORS   Vu TV, Nguyen NT, Kim J, Song YJ, Nguyen TH, Kim JY
  TITLE     Optimized dicot prime editing enables heritable desired edits in 
            tomato and Arabidopsis.
  JOURNAL   Nat Plants. 2024 Sep 6. doi: 10.1038/s41477-024-01786-w.
  PUBMED    39242983
REFERENCE   2  (bases 1 to 14885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2024 Sep 6. doi: 10.1038/s41477-024-01786-w."
COMMENT     Sequence Label: pLSL.R.Ly
FEATURES             Location/Qualifiers
     source          1..14885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    412..436
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             716..1345
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1774..2847
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2913..3107
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        3169..3273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3274..4068
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4239..4827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4728..4747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4963..4987
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      11546..11555
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttattgtga ctccgagggg ttgcctcaaa ctctatctta taaccggcgt ggaggcatgg
       61 aggcaggggt attttggtca ttttaataga tagtggaaaa tgacgtggaa tttacttaaa
      121 gacgaagtct ttgcgacaag ggggggccca cgccgaattt aatattaccg gcgtggcccc
      181 cccttatcgc gagtgcttta gcacgagcgg tccagattta aagtagaaaa tttcccgccc
      241 actagggtta aaggtgttca cactataaaa gcatatacga tgtgatggta tttgatggag
      301 cgtatattgt atcaggtatt tccgttggat acgaattatt cgtacgaccc tcactagagg
      361 atgcacatgt gaccgaggga cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat
      421 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
      481 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgcc agccgtgcgg ctgcatgaaa
      541 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
      601 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
      661 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
      721 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
      781 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
      841 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
      901 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
      961 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     1021 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     1081 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     1141 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     1201 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     1261 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     1321 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     1381 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     1441 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     1501 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     1561 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     1621 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     1681 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa
     1741 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
     1801 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
     1861 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
     1921 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgtcgtc gattaggaag
     1981 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
     2041 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
     2101 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
     2161 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
     2221 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
     2281 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
     2341 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
     2401 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
     2461 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
     2521 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
     2581 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
     2641 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
     2701 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
     2761 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
     2821 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
     2881 gcaggggaaa aaggtcgaaa aagcttcttt cctgtggata gcacgtacat tgggaaccca
     2941 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
     3001 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
     3061 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
     3121 cgcacagccg aacagctgca aaaagcgccc acatcaaggc tccgagtgcg cggaacccct
     3181 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3241 taaatgcttc aataatattg aaaaaggaag agtatggcta aaatgagaat atcaccggaa
     3301 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
     3361 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
     3421 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
     3481 aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc
     3541 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
     3601 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
     3661 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
     3721 gatctggccg atgtggattg cgaaaactgg gaagaggaca ctccatttaa agatccgcgc
     3781 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
     3841 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
     3901 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgctcagg
     3961 gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
     4021 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagct gtcagaccaa
     4081 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4141 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4201 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4261 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4321 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4381 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4441 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4501 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4561 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4621 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4681 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4741 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4801 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4861 ctcggatctg ttggaccgga cagtagtcat ggttgatggg ctgcctgtat cgagtggtga
     4921 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
     4981 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
     5041 gggttgaaca ctctgtgcct agcagaaggc atgttgttgt gactccgagg ggttgcctca
     5101 aactctatct tataaccggc gtggaggcat ggaggcaggg gtattttggt cattttaata
     5161 gatagtggaa aatgacgtgg aatttactta aagacgaagt ctttgcgaca agggggggcc
     5221 cacgccgaat ttaatattac cggcgtggcc cccccttatc gcgagtgctt tagcacgagc
     5281 ggtccagatt taaagtagaa aatttcccgc ccactagggt taaaggtgtt cacactataa
     5341 aagcatatac gatgtgatgg tatttgatgg agcgtatatt gtatcaggta tttccgttgg
     5401 atacgaatta ttcgtacgac cctcggcgcg tgccttgtct tccagtggta tggggtaccg
     5461 cacggtctgc caatccgacg gaggtttatg aattttccac cttttccaca agctcaacta
     5521 gtattaacga tgtggattta gcaaaaaaaa cctgtaaccc taaatgtaaa ataacgggta
     5581 agcctgccaa ccatgttatg gcagattaag cgtctttttg aagggcaccg catctttcgc
     5641 gttgccgtaa atgtatccgt ttagcaataa ggaaattctt aatgaataat ccgtcgttac
     5701 tcaatcatgc ggtcgaaacg atggcagttg gctcgaaaag ttttgcgaca gcctcaaagt
     5761 tatttgatgc aaaaacccgg cgcagcgtac tgatgctcta cgcctggtgc cgccattgtg
     5821 acgatgttat tgacgatcag acgctgggct ttcaggcccg gcagcctgcc ttacaaacgc
     5881 ccgaacaacg tctgatgcaa cttgagatga aaacgcgcca ggcctatgca ggatcgcaga
     5941 tgcacgaacc ggcgtttgcg gcttttcggg aagtggctat ggctcatgat atcgccccgg
     6001 cttacgcgtt tgatcatctg gaaggcttcg ccatggatgt acgcgaagcg caatacagcc
     6061 aactggatga tacgctgcgc tattgctatc acgttgcagg cgttgtcggc ttgatgatgg
     6121 cgcaaatcat gggcgtgcgg gataacgcca cgctggaccg cgcctgtgac cttgggctgg
     6181 catttcagtt gaccaatatt gctcgcgata ttgtggacga tgcgcatgcg ggccgctgtt
     6241 atctgccggc aagctggctg gagcatgaag gtctgaacaa agagaattat gcggcacctg
     6301 aaaaccgtca ggcgctgagc cgtatcgccc gtcgtttggt gcaggaagca gaaccttact
     6361 atttgtctgc cacagccggc ctggcagggt tgcccctgcg ttccgcctgg gcaatcgcta
     6421 cggcgaagca ggtttaccgg aaaataggtg tcaaagttga acaggccggt cagcaagcct
     6481 gggatcagcg gcagtcaacg accacgcccg aaaaattaac gctgctgctg gccgcctctg
     6541 gtcaggccct tacttcccgg atgcgggctc atcctccccg ccctgcgcat ctctggcagc
     6601 gcccgctcta ggcttactat aaggatgttt gcaatgaaac caactacggt aattggtgca
     6661 ggcttcggtg gcctggcact ggcaattcgt ctacaagctg cggggatccc cgtcttactg
     6721 cttgaacaac gtgataaacc cggcggtcgg gcttatgtct acgaggatca ggggtttacc
     6781 tttgatgcag gcccgacggt tatcaccgat cccagtgcca ttgaagaact gtttgcactg
     6841 gcaggaaaac agttaaaaga gtatgtcgaa ctgctgccgg ttacgccgtt ttaccgcctg
     6901 tgttgggagt cagggaaggt ctttaattac gataacgatc aaacccggct cgaagcgcag
     6961 attcagcagt ttaatccccg cgatgtcgaa ggttatcgtc agtttctgga ctattcacgc
     7021 gcggtgttta aagaaggcta tctaaagctc ggtactgtcc cttttttatc gttcagagac
     7081 atgcttcgcg ccgcacctca actggcgaaa ctgcaggcat ggagaagcgt ttacagtaag
     7141 gttgccagtt acatcgaaga tgaacatctg cgccaggcgt tttctttcca ctcgctgttg
     7201 gtgggcggca atcccttcgc cacctcatcc atttatacgt tgatacacgc gctggagcgt
     7261 gagtggggcg tctggtttcc gcgtggcggc accggcgcat tagttcaggg gatgataaag
     7321 ctgtttcagg atctgggtgg cgaagtcgtg ttaaacgcca gagtcagcca tatggaaacg
     7381 acaggaaaca agattgaagc cgtgcattta gaggacggtc gcaggttcct gacgcaagcc
     7441 gtcgcgtcaa atgcagatgt ggttcatacc tatcgcgacc tgttaagcca gcaccctgcc
     7501 gcggttaagc agtccaacaa actgcagact aagcgcatga gtaactctct gtttgtgctc
     7561 tattttggtt tgaatcacca tcatgatcag ctcgcgcatc acacggtttg tttcggcccg
     7621 cgttaccgcg agctgattga cgaaattttt aatcatgatg gcctcgcaga ggacttctca
     7681 ctttatctgc acgcgccctg tgtcacggat tcgtcactgg cgcctgaagg ttgcggcagt
     7741 tactatgtgt tggcgccggt gccgcattta ggcaccgcga acctcgactg gacggttgag
     7801 gggccaaaac tacgcgaccg tatttttgcg taccttgagc agcattacat gcctggctta
     7861 cggagtcagc tggtcacgca ccggatgttt acgccgtttg attttcgcga ccagcttaat
     7921 gcctatcatg gctcagcctt ttctgtggag cccgttctta cccagagcgc ctggtttcgg
     7981 ccgcataacc gcgataaaac cattactaat ctctacctgg tcggcgcagg cacgcatccc
     8041 ggcgcaggca ttcctggcgt catcggctcg gcaaaagcga cagcaggttt gatgctggag
     8101 gatcggatat gagcttttac ccaggagaca gagaatgcaa ccgcattatg atctgattct
     8161 cgtgggggct ggactcgcga atggccttat cgccctgcga cttcagcagc agcaacctga
     8221 tatgcgtatt ttgcttatcg acgccgcacc ccaggcgggc gggaatcata cgtggtcatt
     8281 tcaccacgat gatttgactg agagccaaca tcgttggata gctccgctgg tggttcatca
     8341 ctggcccgac tatcaggtac gctttcccac acgccgtcgt aagctgaaca gcggctactt
     8401 ttgtattact tctcagcgtt tcgctgaggt tttacagcga cagtttggcc cgcacttgtg
     8461 gatggatacc gcggtcgcag aggttaatgc ggaatctgtt cggttgaaaa agggtcaggt
     8521 tatcggtgcc cgcgcggtga ttgacgggcg gggttatgcg gcaaattcag cactgagcgt
     8581 gggcttccag gcgtttattg gccaggaatg gcgattgagt cacccgcatg gtttatcatc
     8641 tccgattatc atggatgcca cggtcgatca gcaaaatggt tatcgcttcg tgtacagcct
     8701 gccgctctcg ccgaccagat tgttaattga agatacgcac tatattgata atgcgacatt
     8761 agatcctgaa tgcgcgcggc aaaatatttg cgactatgcc gcgcaacagg gttggcagct
     8821 tcagacactg ctgcgagaag aacagggcgc cttacccatt actctgtcgg gcaatgccga
     8881 cgcattctgg cagcagcgcc ccctggcctg tagtggatta cgtgccggtc tgttccatcc
     8941 taccaccggc tattcactgc cgctggcggt tgccgtggcc gaccgcctga gtgcacttga
     9001 tgtctttacg tcggcctcaa ttcaccatgc cattacgcat tttgcccgcg agcgctggca
     9061 gcagcagggc tttttccgca tgctgaatcg catgctgttt ttagccggac ccgccgattc
     9121 acgctggcgg gttatgcagc gtttttatgg tttacctgaa gatttaattg cccgttttta
     9181 tgcgggaaaa ctcacgctga ccgatcggct acgtattctg agcggcaagc cgcctgttcc
     9241 ggtattagca gcattgcaag ccattatgac gactcatcgt taagcttcag aggaggaatt
     9301 aagtaatgag ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg
     9361 agttacgact gttgccgaaa gagagtttac cgaaactctg cgacgaactg cgccgctatt
     9421 tactcgacag cgtgagccgt tccagcgggc acttcgcctc cgggctgggc acggtcgaac
     9481 tgaccgtggc gctgcactat gtctacaaca ccccgtttga ccaattgatt tgggatgtgg
     9541 ggcatcaggc ttatccgcat aaaattttga ccggacgccg cgacaaaatc ggcaccatcc
     9601 gtcagaaagg cggtctgcac ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa
     9661 gcgtcgggca ttcatcaacc tccattagtg ccggaattgg tattgcggtt gctgccgaaa
     9721 aagaaggcaa aaatcgccgc accgtctgtg tcattggcga tggcgcgatt accgcaggca
     9781 tggcgtttga agcgatgaat cacgcgggcg atatccgtcc tgatatgctg gtgattctca
     9841 acgacaatga aatgtcgatt tccgaaaatg tcggcgcgct caacaaccat ctggcacagc
     9901 tgctttccgg taagctttac tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg
     9961 tgccgccaat taaagagctg ctcaaacgca ccgaagaaca tattaaaggc atggtagtgc
    10021 ctggcacgtt gtttgaggag ctgggcttta actacatcgg cccggtggac ggtcatgatg
    10081 tgctggggct tatcaccacg ctaaagaaca tgcgcgacct gaaaggcccg cagttcctgc
    10141 atatcatgac caaaaaaggt cgtggttatg aaccggcaga aaaagacccg atcactttcc
    10201 atgccgtgct aaatttgatc cctccagcgg ttgtttgccg aaaagtagcg gcggtttgcc
    10261 gagctattca aaaatctttg gcgactggtt gtgcgaaacg gcagcgaaag acaacaagct
    10321 gatggcgatt actccggcga tgcgtgaagg ttccggcatg gtcgagtttt cacgtaaatt
    10381 cccggatcgc tacttcgacg tggcaattgc cgagcaacat gcggtgacct ttgctgcggg
    10441 tctggcgatt ggtgggtaca aacccattgt cgcgatttac tccactttcc tgcaacgcgc
    10501 ctatgatcag gtgctgcatg acgtggcgat tcaaaagctt ccggtcctgt tcgccatcga
    10561 ccgcgcgggc attgttggtg ctgacggtca aacccatcag ggtgcttttg atctctctta
    10621 cctgcgctgc ataccggaaa tggtcattat gaccccgagc gatgaaaacg aatgtcgcca
    10681 gatgctctat accggctatc actataacga tggcccgtca gcggtgcgct acccgcgtgg
    10741 caacgcggtc ggcgtggaac tgacgccgct ggaaaaacta ccaattggca aaggcattgt
    10801 gaagcgtcgt ggcgggaaac tggcgatcct taactttggt acgctgatgc cagaagcggc
    10861 gaaagtcgcc gaatcgctga acgccacgct ggtcgatatg cgttttgtga aaccgcttga
    10921 tgaagcgtta attctggaaa tggccgccag ccatgaagcg ctggtcaccg tagaagaaaa
    10981 cgccattatg ggcggcgcag gcagcggcgt gaacgaagtg ctgatggccc atcgtaaacc
    11041 agtacccgtg ctgaacattg gcctgccgga cttctttatt ccgcaaggaa ctcaggaaga
    11101 aatgcgcgcc gaactcggcc tcgatgccgc tggtatggaa gccaaaatca aggcctggct
    11161 ggcataagct ttgtgtgagg agatgtcgaa tgagcgcaca tgccctgccc aaggcagatc
    11221 tgaccgccac cagcctgatc gtatcgggcg gcatcatcgc cgcttggctg gccctgcatg
    11281 tgcatgcgct gtggtttctg gacgcagcgg cgcatcccat cctggcgatc gcaaatttcc
    11341 tggggctgac ctggctgtcg gtcggattgt tcatcatcgc gcatgacgcg atgcacgggt
    11401 cggtggtgcc ggggcgtccg cgcgccaatg cggcgatggg ccagcttgtc ctgtggctgt
    11461 atgccggatt ttcgtggcgc aagatgatcg tcaagcacat ggcccatcac cgccatgccg
    11521 gaaccgacga cgaccccgat ttcgaccatg gcggcccggt ccgctggtac gcccgcttca
    11581 tcggcaccta tttcggctgg cgcgaggggc tgcttctgcc cgtcatcgtg acggtctatg
    11641 cgctgatcct tggggatcgc tggatgtacg tggtattctg gccgctgccg tcgatcctgg
    11701 cgtcgatcca gctgttcgtg ttcggcacct ggctgccgca ccgccccggc cacgacgcgt
    11761 tcccggaccg ccacaatgcg cggtcgtcgc ggatcagcga ccccgtgtcg ctgctgacct
    11821 gttttcactt tggcggttat catcacgaac accacctgca cccgacggtg ccgtggtggc
    11881 gcctgcccag cacccgcacc aagggcgaca ccgcataagc ttgagctgag gagattttga
    11941 atgcaaacgg aacacgtcat tttattgaat gcacagggag ttcccacggg tacgctggaa
    12001 aagtatgccg cacacacggc agacacccgc ttacatctcg cgttctccag ttggctgttt
    12061 aatgccaaag gacaattatt agttacccgc cgcgcactga gcaaaaaagc atggcctggc
    12121 gtgtggacta actcggtttg tgggcaccca caactgggag aaagcaacga ggacgcagtg
    12181 atccgccgtt gccgttatga gcttggcgtg gaaattacgc ctcctgaatc tatctatcct
    12241 gactttcgct accgcgccac cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta
    12301 tttgccgcac gcaccactag tgcgttacag atcaatgatg atgaagtgat ggattatcaa
    12361 tggtgtgatt tagcagatgt attacacggt attgatgcaa cgccgtgggc gttcagtccg
    12421 tggatggtga tgcaggcgac aaatcgcgaa gccagaaaac gattatctgc atttacccag
    12481 cttaaataag ctttgcctca ggatttttga aatgacggtc tgcgcaaaaa aacacgttca
    12541 tctcactcgc gatgctgcgg agcagttact ggctgatatt gatcgacgcc ttgatcagtt
    12601 attgcccgtg gagggagaac gggatgttgt gggtgccgcg atgcgtgaag gtgcgctggc
    12661 accgggaaaa cgtattcgcc ccatgttgct gttgctgacc gcccgcgatc tgggttgcgc
    12721 tgtcagccat gacggattac tggatttggc ctgtgcggtg gaaatggtcc acgcggcttc
    12781 gctgatcctt gacgatatgc cctgcatgga cgatgcgaag ctgcggcgcg gacgccctac
    12841 cattcattct cattacggag agcatgtggc aatactggcg gcggttgcct tgctgagtaa
    12901 agcctttggc gtaattgccg atgcagatgg cctcacgccg ctggcaaaaa atcgggcggt
    12961 ttctgaactg tcaaacgcca tcggcatgca aggattggtt cagggtcagt tcaaggatct
    13021 gtctgaaggg gataagccgc gcagcgctga agctattttg atgacgaatc actttaaaac
    13081 cagcacgctg ttttgtgcct cgatgcagat ggcctcgatt gttgcgaatg cctccagcga
    13141 agcgcgtgat tgcctgcatc gtttttcact tgatcttggt caggcatttc aactgctgga
    13201 cgatttgacc gatggcatga ccgacaccgg taaggatagc aatcaggacg ccggtaaatc
    13261 gacgctggtc aatctgttag gcccgagggc ggttgaagaa cgtctgagac aacatcttca
    13321 gcttgccagt gagcatctct ctgcggcctg ccaacacggg cacgccactc aacattttat
    13381 tcaggcctgg tttgacaaaa aactcgctgc cgtcagttaa gcttgcaaga attcaagctt
    13441 ggagtacgtt aattagttgt ctgttttaag ccccgtgctc tccgccgggg cttttttatt
    13501 aaaaaccact caagtggaag acaagggaag ctttgggtac gtcacgtggc tcgagcgcgt
    13561 agtcctcggt aatatcgcag aacaaaagta cctgatatcg agtgtacttc aagtcagtgg
    13621 gaaatcaata aaatgattat tttatgaata tatttcattg tgcaagtaga tagaaattac
    13681 atatgttaca taacacacga aataaacaaa aaaagacaat ccaaaaacaa acaccccaaa
    13741 aaaaataatc actttagata aactcgtatg aggagaggca cgttgcctca gtgactcgac
    13801 gattcccgag caaaaaaagt ctccccgtca cacatgtagt gggtgacgca attatcttta
    13861 aagtaatcct tctgttgact tgtcattgat aacatccagt cctcgtcagg attgcaaaga
    13921 attatagaag ggatcccacc ttttattttc ttcttttttc catatttagg gttgacagtg
    13981 aaatcagact ggcaacctat taattgcttc cacaatggga cgaacttgaa ggggatgtcg
    14041 tcgatgatat tataggtggc gtgttcatcg tagttggtga aatcgatggt accgttccaa
    14101 tagttgtgtc gtccgagact tctagcccag gtggtctttc cggtacgagt tggtccgcag
    14161 atgtagaggc tggggtgtcg gattccattc cttccattgt ccttgttaaa tcggccatcc
    14221 attcaaggtc agattgagct tgttggtatg agacaggatg tatgtaagta taagcgtcta
    14281 tgcttacatg gtatagatgg gtttccctcc aggagtgtag atcttcgtgg cagcgaagat
    14341 ctgattctgt gaagggcgac acatacggtt caggttgtgg agggaataat ttgttggctg
    14401 aatattccag ccattgaagc tttgttgccc attcatgagg gaattcttcc ttgatcatgt
    14461 caagatattc ctccttagac gttgcagtct ggataatagt tctccatcgt gcgtcagatt
    14521 tgcgaggaga aaccttatga tctcggaaat ctcctctggt tttaatatct ccgtcctttg
    14581 atatgtaatc aaggacttgt ttagagtttc tagctggctg gatattaggg tgatttcctt
    14641 caaaatcgaa aaaagaagga tccctaatac aaggtttttt atcaagctgg agaagagcat
    14701 gatagtgggt agtgccatct tgatgaagct cagaagcaac accaaggaag aaaataagaa
    14761 aaggtgtgag tttctcccag agaaactgga ataaatcatc tctttgagat gagcacttgg
    14821 gataggtaag gaaaacatat ttagattgga gtctgaagtt cttactagca gaaggcattt
    14881 tggga
//
