LOCUS       sequence-452447-        3452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a single fluorescent protein, for MuSIC 
            barcode construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye O, Biswas T, 
            Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR
  TITLE     Genetically Encoded Fluorescence Barcodes Allow for Single-Cell 
            Analysis via Spectral Flow Cytometry.
  JOURNAL   ACS Synth Biol. 2025 May 16;14(5):1533-1548. doi: 
            10.1021/acssynbio.4c00807. Epub 2025 May 6.
  PUBMED    40326708
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00807"; journalName: "ACS Synth Biol"; date: 
            "2025-05-16- 16"; volume: "14"; issue: "5"; pages: "1533-1548"
COMMENT     Sequence Label: pReceiver
FEATURES             Location/Qualifiers
     source          1..3452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(114..908)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(114..908)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(225..244)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     835..854
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(943..1139)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(951..1161)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      957..1092
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1185..1205
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1249..1704
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1336..1355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1546..1567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1711..1832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1722..1741)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1776..1795
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(1952..2155)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1981..2001)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2156..2459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2640..3228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2720..2739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3287..3334
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3359..3378
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
       61 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
      121 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
      181 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
      241 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
      301 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
      361 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
      421 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
      481 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
      541 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
      601 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
      661 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
      721 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
      781 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
      841 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
      901 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
      961 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     1021 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     1081 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     1141 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     1201 attccacagc tggttctttc cgcctcagga ctcttccttt aagtactgac gcgccctgta
     1261 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1321 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1381 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1441 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     1501 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     1561 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     1621 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     1681 acaaaatatt aacgcttaca atttacgcct taagatacat tgatgagttt ggacaaacca
     1741 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     1801 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1861 ttcagaattc gagacccagt gggttctcta gttagccaga gggtctcgga tccatttcga
     1921 taagccagta agcacgaggc tgatcagcgg tagctctgct tatatagacc tcccaccgta
     1981 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     2041 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     2101 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     2161 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     2221 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     2281 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     2341 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     2401 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgg
     2461 ctagccggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     2521 aactaatgca tggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2581 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2641 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2701 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2761 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2821 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2881 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2941 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3001 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3061 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3121 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3181 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3241 atcttttcta cggggtctga cgctcagtgg aacgaaccgc gtttatgaac aaacgaccca
     3301 acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt
     3361 gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc cagcatgaga
     3421 tccccgcgct ggaggatcat ccagccggcg tc
//
