LOCUS       sequence-452505-        3061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Medium copy cloning vector with mCherry integrated in the multiple 
            cloning site. There is a SmaI immediately upstream of mCherry start 
            codon, which allows for C-terminal mCherry fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Braun HG, Kanwal N, Rivera Lopez LF, Thomassin JL
  TITLE     Generation of a plasmid series for rapid sub-cloning and use in 
            various Enterobacteriaceae.
  JOURNAL   J Biosci Bioeng. 2024 Sep 6:S1389-1723(24)00253-6. doi: 
            10.1016/j.jbiosc.2024.08.006.
  PUBMED    39244484
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biosci 
            Bioeng. 2024 Sep 6:S1389-1723(24)00253-6. doi: 
            10.1016/j.jbiosc.2024.08.006."
COMMENT     Sequence Label: pSU19mCherry
FEATURES             Location/Qualifiers
     source          1..3061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1072..1094)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1481..1583
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1584..2243
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1650..1669)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2304..2849
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2750..2769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2966..2983
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cactgacacc ctcatcagtg ccaacatagt aagccagtat atacactccg ctagcgccca
       61 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      121 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg
      301 caggtcgact ctagaggatc cccgggatgg tatcaaaagg agaggaagat aatatggcga
      361 tcatcaaaga gttcatgcgt tttaaagtgc acatggaagg ctccgtgaac ggtcacgagt
      421 tcgaaattga gggcgagggc gagggccgtc cgtatgaagg cacgcaaact gctaaactca
      481 aggtcacgaa aggcggacca ctgccttttg cctgggacat cctgtctccg cagtttatgt
      541 acggcagcaa agcgtacgtt aaacatccgg cagatattcc ggactacctg aagttgtcct
      601 tcccggaggg ttttaagtgg gagcgtgtta tgaatttcga ggacggcggt gtcgtgaccg
      661 ttacccaaga cagcagcctg caagatggcg agttcatcta taaagttaag ctgcgtggta
      721 cgaacttccc gtctgatggc ccggtgatgc agaaaaagac catgggttgg gaagcgagca
      781 gcgagcgcat gtacccggaa gacggtgcgc tgaagggtga gatcaaacag cgcctgaagt
      841 tgaaggacgg tggccactac gatgcagaag taaaaaccac ctataaggct aaaaaaccgg
      901 ttcagttgcc gggtgcctat aacgtgaata ttaagttaga catcaccagc cataacgaag
      961 actacaccat tgtggaacaa tatgagcgcg cggagggtcg tcacagcacc ggtggtatgg
     1021 atgaactgta caagtaatga taatagggta ccgagctcga attctggccg tcgttttaca
     1081 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1141 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1201 cagcctgaat ggcgaatggc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc
     1261 gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa
     1321 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
     1381 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
     1441 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
     1501 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     1561 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1621 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1681 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1741 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1801 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1861 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1921 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1981 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     2041 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     2101 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     2161 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     2221 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccta gaaatatttt
     2281 atctgattaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct
     2341 gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc taccaactct
     2401 ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct ttcagtttag
     2461 ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag tggctgctgc
     2521 cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac cggataaggc
     2581 gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta
     2641 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac
     2701 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg ggaaacgcct
     2761 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat
     2821 gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc tcacttccct
     2881 gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag ccatttccgc
     2941 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatatcct
     3001 gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac atgaagcact
     3061 t
//
