LOCUS       sequence-452519-       11347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Capture plasmid with apramycin resistance and pIJ101 replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Xie F, Zhao H, Liu J, Yang X, Neuber M, Agrawal AA, Kaur A, Herrmann
            J, Kalinina OV, Wei X, Muller R, Fu C
  TITLE     Autologous DNA mobilization and multiplication expedite natural 
            products discovery from bacteria.
  JOURNAL   Science. 2024 Dec 13;386(6727):eabq7333. doi: 
            10.1126/science.abq7333. Epub 2024 Dec 13.
  PUBMED    39666857
REFERENCE   2  (bases 1 to 11347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-13"; volume: "386(6727):eabq7333. doi"; pages: " 
            10.1126/science.abq7333. Epub 2024 Dec 13"
COMMENT     Sequence Label: pCap-101-Apr
FEATURES             Location/Qualifiers
     source          1..11347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..474
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(547..1518)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1518..2693)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3019..3269
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3272..4027)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4118..4337
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4405..4936
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4413..5023
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     oriT            8035..8144
                     /note="incP origin of transfer"
     CDS             complement(8474..9274)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      complement(9513..10101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9593..9612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10272..11063,11064..11132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10895..10914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11133..11237)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacaatacaa gtgggaccgt ggtcccagac cgataatcag accgacaaca cgagtgggat
       61 cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat
      121 aatcagaccg acgatacgag tgggaccgtg gttccagact aataatcaga ccgacgatac
      181 gagtgggacc gtggtcccag actaataatc agaccgacga tacgagtggg accatggtcc
      241 cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagtctg attatcagac
      301 cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga
      361 ccgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagtctga
      421 ttatcagacc gacgatacaa gtggaacagt gggcccagag agaatattca ggccagttat
      481 gctttctggc ctgtaacaaa ggacattaag taaagacaga taaacgtaga ctaaaacgtg
      541 gtcgcatcag ggtgctggct tttcaagttc cttaagaatg gcctcaattt tctctataca
      601 ctcagttgga acacgagacc tgtccaggtt aagcaccatt ttatcgccct tatacaatac
      661 tgtcgctcca ggagcaaact gatgtcgtga gcttaaacta gttcttgatg cagatgacgt
      721 tttaagcaca gaagttaaaa gagtgataac ttcttcagct tcaaatatca ccccagcttt
      781 tttctgctca tgaaggttag atgcctgctg cttaagtaat tcctctttat ctgtaaaggc
      841 tttttgaagt gcatcacctg accgggcaga tagttcaccg gggtgagaaa aaagagcaac
      901 aactgattta ggcaatttgg cggtgttgat acagcgggta ataatcttac gtgaaatatt
      961 ttccgcatca gccagcgcag aaatatttcc agcaaattca ttctgcaatc ggcttgcata
     1021 acgctgacca cgttcataag cacttgttgg gcgataatcg ttacccaatc tggataatgc
     1081 agccatctgc tcatcatcca gctcgccaac cagaacacga taatcacttt cggtaagtgc
     1141 agcagcttta cgacggcgac tcccatcggc aatttctatg acaccagata ctcttcgacc
     1201 gaacgccggt gtctgttgac cagtcagtag aaaagaaggg atgagatcat ccagtgcgtc
     1261 ctcagtaagc agctcctggt cacgttcatt acctgaccat acccgagagg tcttctcaac
     1321 actatcaccc cggagcactt caagagtaaa cttcacatcc cgaccacata caggcaaagt
     1381 aatggcatta ccgcgagcca ttactcctac gcgcgcaatt aacgaatcca ccatcggggc
     1441 agctggtgtc gataacgaag tatcttcaac cggttgagta ttgagcgtat gttttggaat
     1501 aacaggcgca cgcttcatta tctaatctcc cagcgtggtt taatcagacg atcgaaaatt
     1561 tcattgcaga caggttccca aatagaaaga gcatttctcc aggcaccagt tgaagagcgt
     1621 tgatcaatgg cctgttcaaa aacagttctc atccggatct gacctttacc aacttcatcc
     1681 gtttcacgta caacattttt tagaaccatg cttccccagg catcccgaat ttgctcctcc
     1741 atccacgggg actgagagcc attactattg ctgtatttgg taagcaaaat acgtacatca
     1801 ggctcgaacc ctttaagatc aacgttcttg agcagatcac gaagcatatc gaaaaactgc
     1861 agtgcggagg tgtagtcaaa caactcagca ggcgtgggaa caatcagcac atcagcagca
     1921 catacgacat taatcgtgcc gatacccagg ttaggcgcgc tgtcaataac tatgacatca
     1981 tagtcatgag caacagtttc aatggccagt cggagcatca ggtgtggatc ggtgggcagt
     2041 ttaccttcat caaatttgcc cattaactca gtttcaatac ggtgcagagc cagacaggaa
     2101 ggaataatgt caagccccgg ccagcaagtg ggctttattg cataagtgac atcgtccttt
     2161 tccccaagat agaaaggcag gagagtgtct tctgcatgaa tatgaagatc tggtacccat
     2221 ccgtgataca ttgaggctgt tccctggggg tcgttacctt ccacgagcaa aacacgtagc
     2281 cccttcagag ccagatcctg agcaagatga acagaaactg aggttttgta aacgccacct
     2341 ttatgggcag caaccccgat caccggtgga aatacgtctt cagcacgtcg caatcgcgta
     2401 ccaaacacat cacgcatatg attaatttgt tcaattgtat aaccaacacg ttgctcaacc
     2461 cgtcctcgaa tttccatatc cgggtgcggt agtcgccctg ctttctcggc atctctgata
     2521 gcctgagaag aaaccccaac taaatccgct gcttcaccta ttctccagcg ccgggttatt
     2581 ttcctcgctt ccgggctgtc atcattaaac tgtgcaatgg cgatagcctt cgtcatttca
     2641 tgaccagcgt ttatgcactg gttaagtgtt tccatgagtt tcattctgaa catcctttaa
     2701 tcattgcttt gcgttttttt attaaatctt gcaatttact gcaaagcaac aacaaaatcg
     2761 caaagtcatc aaaaaaccgc aaagttgttt aaaataagag caacactaca aaaggagata
     2821 agaagagcac atacctcagt cacttattat cactagcgct cgccgcagcc gtgtaaccga
     2881 gcatagcgag cgaactggcg aggaagcaaa gaagaactgt tctgtcagat agctcttacg
     2941 ctcagcgcaa gaagaaatat ccaccgtggg aaaaactcca ggtagaggta cacacgcgga
     3001 tagccaattc agagtaataa actgtgataa tcaaccctca tcaatgatga cgaactaacc
     3061 cccgatatca ggtcacatga cgaagggaaa gagaaggaaa tcaactgtga caaactgccc
     3121 tcaaatttgg cttccttaaa aattacagtt caaaaagtat gagaaaatcc atgcaggctg
     3181 aaggaaacag caaaactgtg acaaattacc ctcagtaggt cagaacaaat gtgacgaacc
     3241 accctcaaat ctgtgacaga taaccctcag actatcctgt cgtcatggaa gtgatatcgc
     3301 ggaaggaaaa tacgatatga gtcgtctggc ggcctttctt tttctcaatg tatgagaggc
     3361 gcattggagt tctgctgttg atctcattaa cacagacctg caggaagcgg cggcggaagt
     3421 caggcatacg ctggtaactt tgaggcagct ggtaacgctc tatgatccag tcgattttca
     3481 gagagacgat gcctgagcca tccggcttac gatactgaca cagggattcg tataaacgca
     3541 tggcatacgg attggtgatt tcttttgttt cactaagccg aaactgcgta aaccggttct
     3601 gtaacccgat aaagaaggga atgagatatg ggttgatatg tacactgtaa agccctctgg
     3661 atggactgtg cgcacgtttg ataaaccaag gaaaagattc atagcctttt tcatcgccgg
     3721 catcctcttc agggcgataa aaaaccactt ccttccccgc gaaactcttc aatgcctgcc
     3781 gtatatcctt actggcttcc gcagaggtca atccgaatat ttcagcatat ttagcaacat
     3841 ggatctcgca gataccgtca tgttcctgta gggtgccatc agattttctg atctggtcaa
     3901 cgaacagata cagcatacgt ttttgatccc gggagagact atatgccgcc tcagtgaggt
     3961 cgtttgactg gacgattcgc gggctatttt tacgtttctt gtgattgata accgctgttt
     4021 ccgccatgac agatccatgt gaagtgtgac aagtttttag attgtcacac taaataaaaa
     4081 agagtcaata agcagggata actttgtgaa aaaacagctt cttctgaggg caatttgtca
     4141 cagggttaag ggcaatttgt cacagacagg actgtcattt gagggtgatt tgtcacactg
     4201 aaagggcaat ttgtcacaac accttctcta gaaccagcat ggataaaggc ctacaaggcg
     4261 ctctaaaaaa gaagatctaa aaactataaa aaaaataatt ataaaaatat ccccgtggat
     4321 aagtggataa ccccaaggga agttttttca ggcatcgtgt gtaagcagaa tatataagtg
     4381 ctgttccctg gtgcttcctc gctcactcga ccgggagggt tcgagaaggg ggggcacccc
     4441 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
     4501 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
     4561 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
     4621 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
     4681 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
     4741 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
     4801 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
     4861 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
     4921 cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
     4981 cagggccata gacggccgcc agcccagcgg cgagggcaac cagccagatc cgaaaacccc
     5041 aagttacgga tctgagggct tcgccctgtc gctcaactgc ggcgagcact actggctgta
     5101 aaaggacaga ccacatcatg gttctgtgtt cattaggttg ttctgtccat tgctgacata
     5161 atccgctcca cttcaacgta acaccgcacg aagatttcta ttgttcctga aggcatattc
     5221 aaatcgtttt cgttaccgct tgcaggcatc atgacagaac actacttcct ataaacgcta
     5281 cacaggctcc tgagattaat aatgcggatc tctacgataa tgggagattt tcccgactgt
     5341 ttcgttcgct tctcagtgga taacagccag cttctctgtt taacagacaa aaacagcata
     5401 tccactcagt tccacatttc catataaagg ccaaggcatt tattctcagg ataattgttt
     5461 cagcatcgca accgcatcag actccggcat cgcaaactgc acccggtgcc gggcagccac
     5521 atccagcgca aaaaccttcg tgtagacttc cgttgaactg atggacttat gtcccatcag
     5581 gctttgcaga actttcagcg gtataccggc atacagcatg tgcatcgcat aggaatggcg
     5641 gaacgtatgt ggtgtgaccg gaacagagaa cgtcacaccg tcagcagcag cggcggcaac
     5701 cgcctcccca atccaggtcc tgaccgttct gtccgtcact tcccagatcc gcgctttctc
     5761 tgtccttcct gtgcgacggt tacgccgctc catgagctta tcgcgaataa atacctgtga
     5821 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
     5881 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
     5941 ataatgaaat aagatcacta ccggagtacc gcctgcacga agtgccggcg gggccgaccc
     6001 cgggcgagta atcccaggat tactcccgcg gcttcgaccc cggccgccgt cgccgcgtac
     6061 gtcaccgacc cccgccgtac gtcaccggga tgacgtacgg cgggggggag cgagttagtg
     6121 cgaagtgggc ccacttgcga gccgggcgat gtgccgggcg gcccgctcct ggcggtcgtc
     6181 ggcgtcgtcg tcctggtcgt cgtcctgctc tcgccgtcgg cgtgcagttg cttcctcgcg
     6241 gcgctgggcg agggcggcga gcatgtcggc gtacgcctcg gccacctccc ccgccgtgag
     6301 caccaccact gtgtcggccg cgtcggccag cgccaggacc tcccgcaccc gttcgcccac
     6361 ggccgccgaa tcctcgttgc cgtccttgcc ttcggcggcc cgggtcgcct cgaggtcgag
     6421 ggcgcggcgg gtgaccgcgt gccatccgtc ctcggtcacg gcgaccccgg cccgcagctc
     6481 cccgccgtcg gcgtcggccg ccaggagcag atcgaggtcg tcggcctcgg tgtcgccgcc
     6541 gtcgagcccg agcatctgcc gcaggtagcg ggtccattcg atggcccggc gtccccgggt
     6601 tgcccgctcg tactcgtgcc agcgcgagag gttccactcc agcgagccga ccccggcggc
     6661 gtcgtcctcg gtcatgccgc cggtcaggtc cccgatccgt ccgaggagtt cgaacggggc
     6721 gacgttcccg ccggtcgccg tcttgaggtc ggcgcgggcg agttcgaggg cgggcgcctt
     6781 cccgtcctgg gtcttggcga tgtactcggc gaggtcgttg gcgtcgcgct cggtctccag
     6841 ccgcttgaag tcgacgccgt gccggtcgtc gggcgtgaag gcggggttga ccttgcgcag
     6901 ggcggcggtc cacacggacc gccagtgccc ctgccactcg tcgagcgcgg cgccggtcgg
     6961 ctcgaaggtg gcgacgatct gcttcgcgga ccgctccccc tcggtccggc cgccgaccag
     7021 gacgatcgcg tggatgtgcg ggtgccagcc gttgatctgc cccacggtga cttcggtcgc
     7081 gcggatcatg ccgacgtacc cgatccggtc tcggatgccc tcgcggtcgg cggcccggtg
     7141 cccgtccttg gcccggcgtc cggcccacgt gccgcccgtg atcagtcgct ggtaggcgcc
     7201 cggccgccgg gggctgtccg gcgtcttccg ggtgccctgg agggcgtcca tgaggtccgc
     7261 gagccggtcc gtgtgccgat ggcgggccgt gaaggtgacc aggtaggcgg tccccccgcg
     7321 cttgatccac tcgaccacgg cggcggtgat ctcctcggcc cgcttgtgcc ggatcgtggc
     7381 ggcgcagacc gggcagagcc agatccgccc gcaccgcatc aggcccagga ccacggacgt
     7441 tccggccgcc gtctgggcga cgatcacgcc ggaggcaggg tccatcaggg cgcggccgca
     7501 gcccttgcac gcggcgtccc cgctgatccg ccacagcgtc cggcggcggc tgtaccgggc
     7561 ggctttccgc agtcgggcag cgtcgctccg cgacgtgctt cctacttccg agaggctgtc
     7621 gcctctcggg ctctccccat ccaccccgtc cggagaaacc gcaggtcgga ggggtgcggg
     7681 aaactctgtt gtttctttcc caaggtgttc gcttttgcct cgggcggcat ctcgcgtcac
     7741 acgcgcgatc gcccgcttcg ctgccatccg gcagcggtct gagcagtaga ggggcgtcgt
     7801 gtaacccacc cagggcgatt gctccggcac ggggaagccg cgccacgcct tcgggacgtc
     7861 tggaatcgct agagcttgca tgcctgcagg tcgacggatc ttttccgctg cataaccctg
     7921 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     7981 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     8041 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     8101 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg ccggcgtaac
     8161 agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca gcccacctat
     8221 caaggtgtac tgccttccag acgaacgaag agcgattgag gaaaaggcgg cggcggccgg
     8281 catgagcctg tcggcctacc tgctggccgt cggccagggc tacaaaatca cgggcgtcgc
     8341 caaacggcat tgagcgtcag catatcatca gcgagctgaa gaaagacaat ccccgatccg
     8401 ctccacgtgt tgccccagca atcagcgcga ccttgcccct ccaacgtcat ctcgttctcc
     8461 gctcatgagc tcagccaatc gactggcgag cggcatcgca ttcttcgcat cccgcctctg
     8521 gcggatgcag gaagatcaac ggatctcggc ccagttgacc cagggctgtc gccacaatgt
     8581 cgcgggagcg gatcaaccga gcaaaggcat gaccgactgg accttccttc tgaaggctct
     8641 tctccttgag ccacctgtcc gccaaggcaa agcgctcaca gcagtggtca ttctcgagat
     8701 aatcgacgcg taccaacttg ccatcctgaa gaatggtgca gtgtctcggc accccatagg
     8761 gaacctttgc catcaactcg gcaagatgca gcgtcgtgtt ggcatcgtgt cccacgccga
     8821 ggagaagtac ctgcccatcg agttcatgga cacgggcgac cgggcttgca ggcgagtgag
     8881 gtggcagggg caatggatca gagatgatct gctctgcctg tggccccgct gccgcaaagg
     8941 caaatggatg ggcgctgcgc tttacatttg gcaggcgcca gaatgtgtca gagacaactc
     9001 caaggtccgg tgtaacgggc gacgtggcag gatcgaacgg ctcgtcgtcc agacctgacc
     9061 acgagggcat gacgagcgtc cctcccggac ccagcgcagc acgcagggcc tcgatcagtc
     9121 caagtggccc atcttcgagg ggccggacgc tacggaagga gctgtggacc agcagcacac
     9181 cgccgggggt aaccccaagg ttgagaagct gaccgatgag ctcggctttt cgccattcgt
     9241 attgcacgac attgcactcc accgctgatg acatcagtcg atcatagcac gatcaacggc
     9301 actgttgcaa atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac
     9361 atgaaccgtt taaacctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10261 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10321 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10381 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10441 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10501 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10561 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10621 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10681 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10741 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10801 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10861 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10921 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10981 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11041 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11101 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11161 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11221 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    11281 attatcatga cattaaccta taaaaatagg cgtatcacgt ttaaactctc acgctgatag
    11341 tctgatc
//
