LOCUS       sequence-452524-       10249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The 3rd generation of rabies vector expressing Flpo recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou 
            Y, Feng G, Wickersham IR
  TITLE     Third-generation rabies viral vectors allow nontoxic retrograde 
            targeting of projection neurons with greatly increased efficiency.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 
            10.1016/j.crmeth.2023.100644.
  PUBMED    37989085
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100644. doi"; pages: "
            10.1016/j.crmeth.2023.100644"
COMMENT     Sequence Label: pRV∆L-5Flpo
FEATURES             Location/Qualifiers
     source          1..10249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     584..606
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..615
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        657..1036
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1037..1240
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1191..1211
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1285..1304
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1285..1303
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6338..6347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6344..6349,6350..6370,6371..7642)
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPo
                     /note="FLPo is a mouse codon-optimized fusion between the 
                     SV40 nuclear localization signal and a thermostable version
                     of the Saccharomyces cerevisiae site-specific recombinase 
                     FLP.  Recombination occurs at FRT sequences."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     primer_bind     complement(7922..7940)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7936..7983
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(8006..8022)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8006..8022)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8059..8079)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8059..8077)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8098..8114)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8098..8114)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8111..8133)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8122..8138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8146..8152,8153..8170,8171..8176))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8191..8212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8329..8346)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8500..9088)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8580..8599)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9259..10050,10051..10119))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9882..9901
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10120..10224)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaaat gagctgattt aacaaaaatt
      421 taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc
      481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      601 aaaacgacgg ccagtgagcg cgcgataagc ttgatatcga attcctgcag cgcgttgaca
      661 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      721 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      781 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      841 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      901 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      961 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1021 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1081 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1141 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1201 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     1261 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
     1321 gattaagcgt ctgatgagtc cgtgaggacg aaacccggcg taccgggtca cgcttaacaa
     1381 ccagatcaaa gaaaaaacag acattgtcaa ttgcaaagca aaaatgtaac acccctacaa
     1441 tggatgccga caagattgta ttcaaagtca ataatcaggt ggtctctttg aagcctgaga
     1501 ttatcgtgga tcaatatgag tacaagtacc ctgccatcaa agatttgaaa aagccctgta
     1561 taaccctagg aaaggctccc gatttaaata aagcatacaa gtcagttttg tcaggcatga
     1621 gcgccgccaa acttaatcct gacgatgtat gttcctattt ggcagcggca atgcagtttt
     1681 ttgaggggac atgtccggaa gactggacca gctatggaat tgtgattgca cgaaaaggag
     1741 ataagatcac cccaggttct ctggtggaga taaaacgtac tgatgtagaa gggaattggg
     1801 ctctgacagg aggcatggaa ctgacaagag accccactgt ccctgagcat gcgtccttag
     1861 tcggtcttct cttgagtctg tataggttga gcaaaatatc cgggcaaaac actggtaact
     1921 ataagacaaa cattgcagac aggatagagc agatttttga gacagcccct tttgttaaaa
     1981 tcgtggaaca ccatactcta atgacaactc acaaaatgtg tgctaattgg agtactatac
     2041 caaacttcag atttttggcc ggaacctatg acatgttttt ctcccggatt gagcatctat
     2101 attcagcaat cagagtgggc acagttgtca ctgcttatga agactgttca ggactggtat
     2161 catttactgg gttcataaaa caaatcaatc tcaccgctag agaggcaata ctatatttct
     2221 tccacaagaa ctttgaggaa gagataagaa gaatgtttga gccagggcag gagacagctg
     2281 ttcctcactc ttatttcatc cacttccgtt cactaggctt gagtgggaaa tctccttatt
     2341 catcaaatgc tgttggtcac gtgttcaatc tcattcactt tgtaggatgc tatatgggtc
     2401 aagtcagatc cctaaatgca acggttattg ctgcatgtgc tcctcatgaa atgtctgttc
     2461 tagggggcta tctgggagag gaattcttcg ggaaagggac atttgaaaga agattcttca
     2521 gagatgagaa agaacttcaa gaatacgagg cggctgaact gacaaagact gacgtagcac
     2581 tggcagatga tggaactgtc aactctgacg acgaggacta cttttcaggt gaaaccagaa
     2641 gtccggaggc tgtttatact cgaatcatga tgaatggagg tcgactaaag agatctcaca
     2701 tacggagata tgtctcagtc agttccaatc atcaagcccg tccaaactca ttcgccgagt
     2761 ttctaaacaa gacatattcg agtgactcat aagaagttga ataacaaaat gccggaaatc
     2821 tacggattgt gtatatccat catgaaaaaa actaacaccc ctcctttcga accatcccaa
     2881 acatgagcaa gatctttgtc aatcctagtg ctattagagc cggtctggcc gatcttgaga
     2941 tggctgaaga aactgttgat ctgatcaata gaaatatcga agacaatcag gctcatctcc
     3001 aaggggaacc catagaggtg gacaatctcc ctgaggatat ggggcgactt cacctggatg
     3061 atggaaaatc gcccaaccat ggtgagatag ccaaggtggg agaaggcaag tatcgagagg
     3121 actttcagat ggatgaagga gaggatccta gcttcctgtt ccagtcatac ctggaaaatg
     3181 ttggagtcca aatagtcaga caaatgaggt caggagagag atttctcaag atatggtcac
     3241 agaccgtaga agagattata tcctatgtcg cggtcaactt tcccaaccct ccaggaaagt
     3301 cttcagagga taaatcaacc cagactactg gccgagagct caagaaggag acaacaccca
     3361 ctccttctca gagagaaagc caatcatcga aagccaggat ggcggctcaa attgcttctg
     3421 gccctccagc ccttgaatgg tcggctacca atgaagagga tgatctatca gtggaggctg
     3481 agatcgctca ccagattgca gaaagtttct ccaaaaaata taagtttccc tctcgatcct
     3541 cagggatact cttgtataat tttgagcaat tgaaaatgaa ccttgatgat atagttaaag
     3601 aggcaaaaaa tgtaccaggt gtgacccgtt tagcccatga cgggtccaaa ctccccctaa
     3661 gatgtgtact gggatgggtc gctttggcca actctaagaa attccagttg ttagtcgaat
     3721 ccgacaagct gagtaaaatc atgcaagatg acttgaatcg ctatacatct tgctaaccga
     3781 acctctcccc tcagtccctc tagacaataa aatccgagat gtcccaaagt caacatgaaa
     3841 aaaacaggca acaccactga taaaatgaac ctcctacgta agatagtgaa aaaccgcagg
     3901 gacgaggaca ctcaaaaatc ctctcccgcg tcagcccctc tggatgacga tgacttgtgg
     3961 cttccacccc ctgaatacgt cccgctgaaa gaacttacag gcaagaagaa catgaggaac
     4021 ttttgtatca acggaagggt taaagtgtgt agcccgaatg gttactcgtt caggatcctg
     4081 cggcacattc tgaaatcatt cgacgagata tattctggga atcataggat gatcgggtta
     4141 gtcaaagtgg ttattggact ggctttgtca ggatctccag tccctgaggg cctgaactgg
     4201 gtatacaaat tgaggagaac ctttatcttc cagtgggctg attccagggg ccctcttgaa
     4261 ggggaggagt tggaatactc tcaggagatc acttgggatg atgatactga gttcgtcgga
     4321 ttgcaaataa gagtgattgc aaaacagtgt catatccagg gcagagtctg gtgtatcaac
     4381 atgaacccga gagcatgtca actatggtct gacatgtctc ttcagacaca aaggtccgaa
     4441 gaggacaaag attcctctct gcttctagaa taatcagatt atatcccgca aatttatcac
     4501 ttgtttacct ctggaggaga gaacatatgg gctcaactcc aacccttggg agcaatataa
     4561 caaaaaacat gttatggtgc cattaaaccg ctgcatttca tcaaagtcaa gttgattacc
     4621 tttacatttt gatcctcttg gatgtgaaaa aaactattaa catccctcaa aagaggcgcg
     4681 ccaaggaaag atggttcctc aggctctcct gtttgtaccc cttctggttt ttccattgtg
     4741 ttttgggaaa ttccctattt acacgatacc agacaagctt ggtccctgga gtccgattga
     4801 catacatcac ctcagctgcc caaacaattt ggtagtggag gacgaaggat gcaccaacct
     4861 gtcagggttc tcctacatgg aacttaaagt tggatacatc ttagccataa aagtgaacgg
     4921 gttcacttgc acaggcgttg tgacggaggc tgaaacctac actaacttcg ttggttatgt
     4981 cacaaccacg ttcaaaagaa agcatttccg cccaacacca gatgcatgta gagccgcgta
     5041 caactggaag atggccggtg accccagata tgaagagtct ctacacaatc cgtaccctga
     5101 ctaccgctgg cttcgaactg taaaaaccac caaggagtct ctcgttatca tatctccaag
     5161 tgtggcagat ttggacccat atgacagatc ccttcactcg agggtcttcc ctagcgggaa
     5221 gtgctcagga gtagcggtgt cttctaccta ctgctccact aaccacgatt acaccatttg
     5281 gatgcccgag aatccgagac tagggatgtc ttgtgacatt tttaccaata gtagagggaa
     5341 gagagcatcc aaagggagtg agacttgcgg ctttgtagat gaaagaggcc tatataagtc
     5401 tttaaaagga gcatgcaaac tcaagttatg tggagttcta ggacttagac ttatggatgg
     5461 aacatgggtc tcgatgcaaa catcaaatga aaccaaatgg tgccctcccg ataagttggt
     5521 gaacctgcac gactttcgct cagacgaaat tgagcacctt gttgtagagg agttggtcag
     5581 gaagagagag gagtgtctgg atgcactaga gtccatcatg acaaccaagt cagtgagttt
     5641 cagacgtctc agtcatttaa gaaaacttgt ccctgggttt ggaaaagcat ataccatatt
     5701 caacaagacc ttgatggaag ccgatgctca ctacaagtca gtcagaactt ggaatgagat
     5761 cctcccttca aaagggtgtt taagagttgg ggggaggtgt catcctcatg tgaacggggt
     5821 gtttttcaat ggtataatat taggacctga cggcaatgtc ttaatcccag agatgcaatc
     5881 atccctcctc cagcaacata tggagttgtt ggaatcctcg gttatccccc ttgtgcaccc
     5941 cctggcagac ccgtctaccg ttttcaagga cggtgacgag gctgaggatt ttgttgaagt
     6001 tcaccttccc gatgtgcaca atcaggtctc aggagttgac ttgggtctcc cgaactgggg
     6061 gaagtatgta ttactgagtg caggggccct gactgccttg atgttgataa ttttcctgat
     6121 gacatgttgt agaagagtca atcgatcaga acctacgcaa cacaatctca gagggacagg
     6181 gagggaggtg tcagtcactc cccaaagcgg gaagatcata tcttcatggg aatcacacaa
     6241 gagtgggggt gagaccagac tgtaaggtac cggactggcc gtcctttcaa cgatccaagt
     6301 ccatgaaaaa aactaacacc cctccgctag caaagccgcc accatggctc ctaagaagaa
     6361 gaggaaggtg atgagccagt tcgacatcct gtgcaagacc ccccccaagg tgctggtgcg
     6421 gcagttcgtg gagagattcg agaggcccag cggcgagaag atcgccagct gtgccgccga
     6481 gctgacctac ctgtgctgga tgatcaccca caacggcacc gccatcaaga gggccacctt
     6541 catgagctac aacaccatca tcagcaacag cctgagcttc gacatcgtga acaagagcct
     6601 gcagttcaag tacaagaccc agaaggccac catcctggag gccagcctga agaagctgat
     6661 ccccgcctgg gagttcacca tcatccctta caacggccag aagcaccaga gcgacatcac
     6721 cgacatcgtg tccagcctgc agctgcagtt cgagagcagc gaggaggccg acaagggcaa
     6781 cagccacagc aagaagatgc tgaaggccct gctgtccgag ggcgagagca tctgggagat
     6841 caccgagaag atcctgaaca gcttcgagta caccagcagg ttcaccaaga ccaagaccct
     6901 gtaccagttc ctgttcctgg ccacattcat caactgcggc aggttcagcg acatcaagaa
     6961 cgtggacccc aagagcttca agctggtgca gaacaagtac ctgggcgtga tcattcagtg
     7021 cctggtgacc gagaccaaga caagcgtgtc caggcacatc tactttttca gcgccagagg
     7081 caggatcgac cccctggtgt acctggacga gttcctgagg aacagcgagc ccgtgctgaa
     7141 gagagtgaac aggaccggca acagcagcag caacaagcag gagtaccagc tgctgaagga
     7201 caacctggtg cgcagctaca acaaggccct gaagaagaac gccccctacc ccatcttcgc
     7261 tatcaagaac ggccctaaga gccacatcgg caggcacctg atgaccagct ttctgagcat
     7321 gaagggcctg accgagctga caaacgtggt gggcaactgg agcgacaaga gggcctccgc
     7381 cgtggccagg accacctaca cccaccagat caccgccatc cccgaccact acttcgccct
     7441 ggtgtccagg tactacgcct acgaccccat cagcaaggag atgatcgccc tgaaggacga
     7501 gaccaacccc atcgaggagt ggcagcacat cgagcagctg aagggcagcg ccgagggcag
     7561 catcagatac cccgcctgga acggcatcat cagccaggag gtgctggact acctgagcag
     7621 ctacatcaac aggcggatct gatgacgtac gaccggatac tcctggaagc ctgcccatgc
     7681 taagactctt gtgtgatgta tcttgaaaaa aacaagatcc taaatctgaa cctttggttg
     7741 tttgattgtt tttctcattt ttgttgttta tttgttaagc gtgggtcggc atggcatctc
     7801 cacctcctcg cggtccgacc tgggcatccg aaggaggacg cacgtccact cggatggcta
     7861 agggagggcg gggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     7921 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     7981 ttgctgaaag gaggaactat atccggatcc actagttcta gagcggccgc caccgcggtg
     8041 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     8101 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8161 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8221 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8281 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8581 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8641 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8701 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8761 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8821 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8881 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8941 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9001 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9061 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9121 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9181 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9241 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9301 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9361 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccaccct tggaggctcc
     9421 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9481 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9541 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9601 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9661 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9721 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9781 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9841 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9901 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9961 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10021 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10081 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10141 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10201 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacct
//
