LOCUS       sequence-452562-        8975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5G repeat, NGFR in p20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Hamilton N, Claudio NM, Armstrong RJ, Pucci F
  TITLE     Cell Surface Labeling by Engineered Extracellular Vesicles.
  JOURNAL   Adv Biosyst. 2020 Dec;4(12):e2000007. doi: 10.1002/adbi.202000007. 
            Epub 2020 May 10.
  PUBMED    32390342
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv 
            Biosyst."; date: "2020-12"; volume: "4(12):e2000007. doi"; pages: " 
            10.1002/adbi.202000007. Epub 2020 May 10"
COMMENT     Sequence Label: p38.pA.OFP.mCMV.hPGK.G5-Flag-NGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..8975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      112..247
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(267..286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(268..286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(296..313)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(296..312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(305..327)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      454..909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(541..560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     751..772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        935..1039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1040..1108,1109..1900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1258..1277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2071..2659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2560..2579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2813..2830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2947..2968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2983..2988,2989..3006,3007..3013)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3021..3037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3026..3048
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3045..3061
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3045..3061
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3080..3100
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3082..3100
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        3201..3580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3581..3784
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3735..3755
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3799..3979
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4026..4151
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4644..4877
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5062..5106
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5373..5490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    5627..5761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5799..6509)
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     promoter        6550..7060
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6979..6997
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      7069..7078
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7174..7197
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8043..8063)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8461..8472)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8621..8854
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    join(8926..8975,1..72)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
       61 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
      121 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      181 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      241 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
      301 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      361 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      421 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      661 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      781 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      841 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      901 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      961 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1021 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1081 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1141 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1201 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1261 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1321 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1381 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1441 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1501 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1561 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1621 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1681 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1741 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1801 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1861 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1921 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1981 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2041 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2101 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2161 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2221 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2281 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2341 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2401 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2461 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2521 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2581 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2641 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2701 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2761 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2821 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2881 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2941 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3001 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     3061 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     3121 agcttggcca ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg
     3181 tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     3241 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3301 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3361 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3421 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3481 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3541 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     3601 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     3661 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     3721 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     3781 agctcgttta gtgaaccggg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3841 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3901 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3961 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     4021 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     4081 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     4141 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     4201 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     4261 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     4321 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     4381 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     4441 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     4501 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     4561 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     4621 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     4681 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     4741 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     4801 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     4861 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     4921 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     4981 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     5041 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     5101 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     5161 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     5221 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     5281 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     5341 ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg
     5401 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     5461 caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgagagatct
     5521 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     5581 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     5641 agcttataat ggttacaaat aaggcaatag catcacaaat ttcacaaata aggcattttt
     5701 ttcactgcat tctagttttg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     5761 ctcaaatccc tcggaagctg cgcctgtcat cggctagctt acttgtacag ctcgtccatg
     5821 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
     5881 ttgtgggagg tgatgtccaa cttgatgtcg acgatgtagg cgccgggcag ctgcacgggc
     5941 ttcttggcct tgtaggtggt cttgacctcg gaggtgtagt ggccgccgtc cttcagcttc
     6001 agcctcatct tgatcttgcc cttcagggca ccgtcctcgg ggtacatccg ctcggaggag
     6061 gcctcccagc ccatggtctt cttctgcatc acggggccgt cggaggggaa gttggtgccg
     6121 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
     6181 accacgccgc cgtcctcgta gttcatcacg cgctcccact tgaagccctc ggggaaggac
     6241 agcttgaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtaggtg
     6301 aaatgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc
     6361 ttagcggtct gaaagccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
     6421 tggccgttca cggagccctc catgcgcacc ttgaagcgca tgaactcctt gatgatggcc
     6481 atgttattct cctcgccctt gctcaccatg cggccgccag tgtgatggat gctcgacttc
     6541 gaattccacg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     6601 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     6661 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     6721 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     6781 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     6841 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
     6901 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     6961 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     7021 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccgc caccatgggt
     7081 gccggcgcca ccggtagggc tatggatggc cctaggctcc tcttgctgct tttgttgggg
     7141 gtgtcattgg gcggtgccgg aggcggaggc ggagactaca aggatgacga cgataagggt
     7201 tccggcttgg aaaaggaagc ctgtcctact ggactctaca cacacagtgg tgaatgctgc
     7261 aaagcatgta accttggtga gggcgtagct cagccctgtg gcgcaaacca aaccgtatgt
     7321 gagccatgtc tggactccgt taccttttcc gatgtcgtaa gtgctaccga gccctgtaaa
     7381 ccctgcactg aatgtgttgg actgcaaagc atgtcagcac cttgcgtaga ggctgatgat
     7441 gccgtgtgcc ggtgcgcata tggatattat caggacgaga ccacaggtcg atgcgaagca
     7501 tgtcgggtct gtgaggccgg aagtggattg gtatttagtt gccaagacaa acagaacact
     7561 gtgtgcgaag aatgtcccga tggtacttat agcgatgagg ccaatcatgt agatccttgc
     7621 ttgccatgta ctgtatgcga agataccgag agacaactgc gcgaatgtac tcggtgggca
     7681 gacgctgaat gtgaagaaat tccagggaga tggataaccc gatccacccc tccagagggg
     7741 tcagacagta cagctccctc aacacaggaa ccagaggccc ctccagagca ggacctgata
     7801 gcatcaaccg tggcaggcgt cgtgacaacc gttatgggaa gcagccagcc agtcgtcacc
     7861 agaggcacca cagacaatct tataccagtg tactgctcaa tcttggcagc agtcgtagtc
     7921 ggcttggtag cctacatagc ctttaagcgc tggaatcgcg ggatattgta gggatccgcc
     7981 gatgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     8041 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     8101 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     8161 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     8221 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     8281 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     8341 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     8401 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8461 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct agagcctctt
     8521 ccgcgtcttc gccttcccgg gtcgagctcg gtacctttaa gaccaatgac ttacaaggca
     8581 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     8641 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     8701 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8761 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8821 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8881 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     8941 gcttataatg gttacaaata aagcaatagc atcac
//
