LOCUS       sequence-45264-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Yuan Y, Cao P, Smith MA, Kramp K, Huang Y, Hisamoto N, Matsumoto K, 
            Hatzoglou M, Jin H, Feng Z
  TITLE     Dysregulated LRRK2 signaling in response to endoplasmic reticulum 
            stress leads to dopaminergic neuron degeneration in C. elegans.
  JOURNAL   PLoS One. 2011;6(8):e22354. Epub 2011 Aug 3.
  PUBMED    21857923
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8)"; pages: "e22354. Epub 2011 Aug 3"
COMMENT     Sequence Label: pcDNA3.1-subtilase A
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             119..136
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             140..172
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(176..184,185..199)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttaccatgg ggggttctca
      121 tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct
      181 gtacgacgat gacgataagg tacctaagga tccatgctga aaattctgtg gacctacatt
      241 ctgttcctgc tgtttatctc tgcttctgct cgtgctgaaa aaccgtggta ctttgatgcg
      301 atcggcctga ccgaaaccac gatgagcctg accgataaaa acacgccggt ggttgtcagc
      361 gtggttgact ctggtgttgc atttattggc ggtctgtcag attcggaatt cgctaaattt
      421 tcattcaccc aggacggctc gccgtttccg gtgaaaaaat cagaagcgct gtacattcat
      481 ggtacggcaa tggctagtct gatcgcatcc cgttatggca tttacggtgt ttatccgcac
      541 gcgctgatca gctctcgtcg cgtgattccg gatggcgttc aggactcttg gattcgcgcg
      601 atcgaaagta ttatgtccaa cgtgttcctg gccccgggcg aagaaaaaat tatcaatatc
      661 tcaggcggtc aaaaagg
//
