LOCUS       sequence-45267-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-GEX-GECO1
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             75..86
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             241..258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(298..306,307..321)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             349..405
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     618..639
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
       61 ttaaccatgg gtttaaagag gagaaaggtc atgaacaata acgatctctt tcaggcatca
      121 cgtcggcgtt ttctggcaca actcggcagc ttaaccgacg ccgggacgct ggggccgtca
      181 ttgttaacgc cgcgacgtgc gactgcggcg caagcggcga ctgacgcttc tagaggttct
      241 catcatcatc atcatcatgg tatggctagc atgacgggtg gacagcaaaa gggtcgggat
      301 ctgtacgacg atgatgataa ggatctcgcc acaatggtcg actcatcacg tcgtaagtgg
      361 aataagacag gtcacgcagt cagagctata ggtcggctga gctcactcga gaacgtctat
      421 atcaaggccg acgagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
      481 gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat cggcgacggc
      541 cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccatactttc gaaagacccc
      601 aacgagaagc gtgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      661 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag
      721 ctgttcaccg gggtggtgcc catccaggtc gagctggacg gcgacgtaaa cggccacaag
      781 ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg gcaagctgac cctgaagttc
      841 atctgcacca ccggcaagct gcccgtg
//
