LOCUS       sequence-45281-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt5B
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..57)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttactaaccg gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc
       61 gaaccgcggg ccctctagat caaccacttt gtacaagaaa gctgggtcgg cgcgcccacc
      121 cttcagaatt catttacaga tgtactggtc cacgatctcc gtgcacttct tacacctgac
      181 gaagcagcac cagtggaact tgcagtggca gcgctccacc tgcacgctct tgaactggtt
      241 gtagccacgc ccgcagcaca tgagctcaca gccatccatg ccctccgagg tcttgttgca
      301 gaggcggccc tgcgtgccca gggagcccgt gctctcgttg cgcaggcagt agtcggggct
      361 ggggtccaca tagaccaggt cctccggggt gggctgggtg aagcggctgt tgaccagctc
      421 cagccggccc ttgcgggtga cgcgcatggc ggccgcgctg tcgtacttct ccttcagccg
      481 gtccccgacc ttgcggaact cggccagctg cagccagcag gtcttgaggc tgcaggaccc
      541 cgagacgccg tggcatttgc aggctacgtc tgccatctta tacacagccc tgcgaccggc
      601 ctcgttgttt tgcaggttca tgagcacccg gccctgctcc tctgatcctt tggcaaagtt
      661 cttctctcgc tcccgggcat ccacaaactc cttggcgaag cggtagccgt actccacgtt
      721 gtccccacag ccgccccaca gccagtcccg gggcaggtcc ttgggccgcg ccgtccggct
      781 gcagccgcag gtggagagct cgccctcgcg gcaggcccgg ctgatggcgt tgaccacgcc
      841 cgcggcgctc accgcgtggg tgaaggcggt ctctcggctg cctatctgca tgactc
//
