LOCUS       sequence-452867-        5121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0050m_3xC2 is an enhancer sequence, designed to drive 
            AAV-mediated transgene expression in specific populations of brain 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP13775 - pAAV-AiE0050m_3xC2-minBG-iCre-BGHpA...
FEATURES             Location/Qualifiers
     source          1..5121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     CDS             1072..1092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2161..2272
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2384..2524
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2384..2513
                     /label=AAV2 ITR
     rep_origin      2599..3054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2686..2705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2896..2917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3071..3090)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3190..3212
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3250..3268)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3336..3440
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3441..3509,3510..4301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3659..3678)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4472..5060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4961..4980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaggca acttgttaaa aatctcagtc
      181 aaacttacat actatataga acagcatggt gaatttaggg cacatggata taaaatggaa
      241 gtttcttatt cagtagcagc aacttgtggg cacaggagtt ggcaaagata aaaatgtcca
      301 aagtcacaaa tacaatgtat agttagtcat aggtgctgtt atttgcctca aaaaatagac
      361 ttttattttg cctttctttt ctttaaccac actcaaaatt agagaacaga gacaaaaccc
      421 agcaggaaat aggcaacttg ttaaaaatct cagtcaaact tacatactat atagaacagc
      481 atggtgaatt tagggcacat ggatataaaa tggaagtttc ttattcagta gcagcaactt
      541 gtgggcacag gagttggcaa agataaaaat gtccaaagtc acaaatacaa tgtatagtta
      601 gtcataggtg ctgttatttg cctcaaaaaa tagactttta ttttgccttt cttttcttta
      661 accacactca aaattagaga acagagacaa aacccagcag gaaataggca acttgttaaa
      721 aatctcagtc aaacttacat actatataga acagcatggt gaatttaggg cacatggata
      781 taaaatggaa gtttcttatt cagtagcagc aacttgtggg cacaggagtt ggcaaagata
      841 aaaatgtcca aagtcacaaa tacaatgtat agttagtcat aggtgctgtt atttgcctca
      901 aaaaatagac ttttattttg cctttctttt ctttaaccac actcaaaatt agagaacaga
      961 gacaaaaccc agcaggaaat gagctcgggc tgggcataaa agtcagggca gagccatcta
     1021 ttgcttacat ttgcttctgg gatccagatc ttcgaagcta gcaccatggt gcccaagaag
     1081 aagaggaaag tctccaacct gctgactgtg caccaaaacc tgcctgccct ccctgtggat
     1141 gccacctctg atgaagtcag gaagaacctg atggacatgt tcagggacag gcaggccttc
     1201 tctgaacaca cctggaagat gctcctgtct gtgtgcagat cctgggctgc ctggtgcaag
     1261 ctgaacaaca ggaaatggtt ccctgctgaa cctgaggatg tgagggacta cctcctgtac
     1321 ctgcaagcca gaggcctggc tgtgaagacc atccaacagc acctgggcca gctcaacatg
     1381 ctgcacagga gatctggcct gcctcgccct tctgactcca atgctgtgtc cctggtgatg
     1441 aggagaatca gaaaggagaa tgtggatgct ggggagagag ccaagcaggc cctggccttt
     1501 gaacgcactg actttgacca agtcagatcc ctgatggaga actctgacag atgccaggac
     1561 atcaggaacc tggccttcct gggcattgcc tacaacaccc tgctgcgcat tgccgaaatt
     1621 gccagaatca gagtgaagga catctcccgc accgatggtg ggagaatgct gatccacatt
     1681 ggcaggacca agaccctggt gtccacagct ggtgtggaga aggccctgtc cctgggggtt
     1741 accaagctgg tggagagatg gatctctgtg tctggtgtgg ctgatgaccc caacaactac
     1801 ctgttctgcc gggtcagaaa gaatggtgtg gctgcccctt ctgccacctc ccaactgtcc
     1861 acccgggccc tggaagggat ctttgaggcc acccaccgcc tgatctatgg tgccaaggat
     1921 gactctgggc agagatacct ggcctggtct ggccactctg ccagagtggg tgctgccagg
     1981 gacatggcca gggctggtgt gtccatccct gaaatcatgc aggctggtgg ctggaccaat
     2041 gtgaacattg tgatgaacta catcagaaac ctggactctg agactggggc catggtgagg
     2101 ctgctcgagg atggggacta atgaggcgcg ccgaattcaa gcttctcgag agatcttcga
     2161 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2221 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     2281 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     2341 gggaagacaa tagcaggcat gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     2401 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     2461 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     2521 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2581 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2641 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     2701 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     2761 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     2821 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     2881 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2941 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3001 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     3061 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3121 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3181 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3241 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3301 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3361 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3421 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3481 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3541 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3601 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3661 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3721 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3781 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3841 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3901 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3961 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4021 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4081 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4141 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4201 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4261 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4321 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4381 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4441 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4501 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4561 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4621 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4681 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4741 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4801 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4861 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4921 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4981 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5041 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5101 gctggccttt tgctcacatg t
//
