LOCUS       sequence-45288-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Eberhardt M, Salmon P, von Mach MA, Hengstler JG, Brulport M, 
            Linscheid P, Seboek D, Oberholzer J, Barbero A, Martin I, Muller B, 
            Trono D, Zulewski H
  TITLE     Multipotential nestin and Isl-1 positive mesenchymal stem cells 
            isolated from human pancreatic islets.
  JOURNAL   Biochem Biophys Res Commun. 2006 Jul 7;345(3):1167-76. Epub 2006 May
            11.
  PUBMED    16713999
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2006-07-7"; volume: "345(3)"; pages: 
            "1167-76. Epub 2006 May 11"
COMMENT     Sequence Label: CX-nestinGFPas
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        211..229
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     211..228
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             254..434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    454..487
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     631..647
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     631..647
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gctgattggt tcgctgacca tttccgggtg cgggacggcg ttaccagaaa ctcagaaggt
       61 tcgtccaacc aaaccgactc tgacggcagt ttacgagaga gatgataggg tctgcttcag
      121 taagccagat gctacacaat taggcttgta catattgtcg ttagaacgcg gctacaatta
      181 atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa tacacggaat
      241 taattctaga tgctgctaga gattttccac actgactaaa agggtctgag ggatctctag
      301 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
      361 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
      421 aaccagagag acccagtaca agcaaaaagc agcataactt cgtataatgt atgctatacg
      481 aagttattcc catggtgaat tgatcccatc ttgtcttcgt tgggagtgaa ttagcccttc
      541 cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg
      601 tcttaaaggt accgagctcg aattggccgc tctagaacta gtggatcccc cgggctgcag
      661 gaattcggcc tatgtggatg tctgtgcatg cagtgaaggc cgacaaggac actggatcct
      721 ctgaagttcc tgccacctgt gagctgccgt gtggacaatg ggaattggca ctcaggtcct
      781 ctgaaggagc agccagtgtg ctcttaacat ctgagcaatt tctccacctg ctcccgaaag
      841 gatatagagt caagaatgtg ggatttattt atttattttg agacagggtc tctctaaata
      901 gt
//
