LOCUS       sequence-45295-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Eberhardt M, Salmon P, von Mach MA, Hengstler JG, Brulport M, 
            Linscheid P, Seboek D, Oberholzer J, Barbero A, Martin I, Muller B, 
            Trono D, Zulewski H
  TITLE     Multipotential nestin and Isl-1 positive mesenchymal stem cells 
            isolated from human pancreatic islets.
  JOURNAL   Biochem Biophys Res Commun. 2006 Jul 7;345(3):1167-76. Epub 2006 May
            11.
  PUBMED    16713999
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2006-07-7"; volume: "345(3)"; pages: 
            "1167-76. Epub 2006 May 11"
COMMENT     Sequence Label: CX-nestinGFPas
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             548..577
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             551..580
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             554..583
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             557..586
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             560..589
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             563..592
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             566..595
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             569..598
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             572..601
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             575..604
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             578..607
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcgaccggt ggatctcgac tggtctggcc cccgagggca
       61 agcggacgtg gagcactaga gaagggagtg gcccgtggag cttttagggg ttgaaaagaa
      121 gtagcggcga cagtgaccca gcggaagcgg agaactccag cagggagctg gagcgagggt
      181 attcatactc ccgccggctg ggcggaaaag gggcggcccc ccaggcctta accctttagc
      241 gttcagtgca acacgcaacc ggggttcctg cttcatccgg gtgcagaaga gaagaaccaa
      301 agtgcagccc ctccctgagg gatccggtcc ggtgcgggtg attttctgag agaatcttga
      361 gatacagcca gacaccccgg aacaccctat gatgcacaga actgggcctg cggacctcct
      421 ttctctacac actcaaattc tctgagactc ggcttcttat ttatagaatg gcttggtgtt
      481 ggaatcctca tgaactgttg gaaaaacgga cggtgcagtg ttttgtgtaa aacagtaaat
      541 attcacacac caccaccacc accaccacca ccaccaccac caccaccacc accaccacca
      601 ccaccacctg tccccaggga ggcaacattg cagagagaca ctgcccgaag caagaaaaac
      661 ccactggtac tgggttttcc tgagctccga agcccgaaga aggattaagg ccagcctggg
      721 cagtaaacac cccccacccc tttcttcaga tggttggtat cgctatgtga atcaggctgg
      781 tctcgaaatc gtagagatgc tcctgctttt
//
