LOCUS       sequence-45296-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Cortesio CL, Chan KT, Perrin BJ, Burton NO, Zhang S, Zhang ZY, 
            Huttenlocher A
  TITLE     Calpain 2 and PTP1B function in a novel pathway with Src to regulate
            invadopodia dynamics and breast cancer cell invasion.
  JOURNAL   J Cell Biol. 2008 Mar 10;180(5):957-71.
  PUBMED    18332219
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2008-03-10"; volume: "180(5)"; pages: "957-71"
COMMENT     Sequence Label: pTrcHisA-rat-PTP1B
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             84..110
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             126..143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             147..179
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(183..191,192..206)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 acaatttatc agacaatctg tgtgggcact cgaccggaat tatcgattaa ctttattatt
       61 aaaaattaaa gaggtatata ttaatgtatc gattaaataa ggaggaataa accatggggg
      121 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc
      181 gggatctgta cgacgatgac gataaggatc gatggggatc catggaaatg gagaaggaat
      241 tcgagcagat cgataaggct gggaactggg cggctattta ccaggatatt cgacatgaag
      301 ccagtgactt cccatgcaga atagcgaaac ttcctaagaa caaaaaccgg aacaggtacc
      361 gagatgtcag cccttttgac cacagtcgga ttaaattgca tcaggaagat aatgactata
      421 tcaatgccag cttgataaaa atggaggaag cccagaggag ctatatcctc acccagggcc
      481 ctttaccaaa cacgtgcggg cacttctggg agatggtgtg ggagcagaag agcaggggcg
      541 tggtcatgct caaccgcatc atggagaaag gctcgttaaa atgtgcccag tattggccac
      601 agaaagaaga aaaagagatg gtcttcgatg acaccaattt gaagctgaca ctgatctctg
      661 aagatgtcaa gtcatattac acagtacggc agttggagtt ggagaacctg gctacccagg
      721 aggctcgaga gatcctgcat ttccactaca ccacctggcc tgactttgga gtccctgagt
      781 cacctgcctc tttcctcaat ttcctattca aagtccgaga gtcaggctca ctcagcccag
//
