LOCUS       sequence-45308-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pNN_v2
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(119..138)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        205..296
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctggcagttc cctactctcg catggggagt ccccacacta ccatcggcgc tacggcgttt
       61 cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaaca
      121 aggggtgtta tgagccatat tcaggtataa atgggctcgc gataatgttc agaattggtt
      181 aattggttgt aacactgacc cctatttgtt tatttttcta aatacattca aatatgtatc
      241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagaatatga
      301 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
      361 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
      421 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
      481 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcca cttccgacca
      541 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
      601 aaacagcgtt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
      661 tggcagtgtt cctgcgccgg ttgcactcga ttcctgtttg taattgtcct tttaacagcg
      721 atcgcgtatt tcgcctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
      781 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
      841 aacttttg
//
