LOCUS       sequence-45310-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Cortesio CL, Chan KT, Perrin BJ, Burton NO, Zhang S, Zhang ZY, 
            Huttenlocher A
  TITLE     Calpain 2 and PTP1B function in a novel pathway with Src to regulate
            invadopodia dynamics and breast cancer cell invasion.
  JOURNAL   J Cell Biol. 2008 Mar 10;180(5):957-71.
  PUBMED    18332219
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2008-03-10"; volume: "180(5)"; pages: "957-71"
COMMENT     Sequence Label: pCDNA 3.1 Flag-rat-PTP1B
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttggtaccg agctcggatc cgccaccatg gactacaagg acgacgatga caagaagctt
      121 gggatggaaa tggagaagga attcgagcag atcgataagg ctgggaactg ggcggctatt
      181 taccaggata ttcgacatga agccagtgac ttcccatgca gaatagcgaa acttcctaag
      241 aacaaaaacc ggaacaggta ccgagatgtc agcccttttg accacagtcg gattaaattg
      301 catcaggaag ataatgacta tatcaatgcc agcttgataa aaatggagga agcccagagg
      361 agctatatcc tcacccaggg ccctttacca aacacgtgcg ggcacttctg ggagatggtg
      421 tgggagcaga agagcagggg cgtggtcatg ctcaaccgca tcatggagaa aggctcgtta
      481 aaatgtgccc agtattggcc acagaaagaa gaaaaagaga tggtcttcga tgacaccaat
      541 ttgaagctga cactgatctc tgaagatgtc aagtcatatt acacagtacg gcagttggag
      601 ttggagaacc tggctaccca ggaggctcga gagatcctgc atttccacta caccacctgg
      661 cctgactttg gagtccctga gtcacctgcc tctttcctca atttcctatt caaagtccga
      721 gagtcaggct cactcagccc agagcacggc cccattgtgg tccactgcag tgctggcatt
      781 ggcaggtcag ggaccttctg cctggctgac acctgcctct tactgatgga caagaggaaa
      841 gacccgtcct ctgtggacat ca
//
