LOCUS       sequence-45324-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: MCHR1B_R (TAL 3009)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..55)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(71..658)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gatgaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga
       61 agggccctta aaagtttatc tcgccgttat taaatttccg tctgacttcc tctaaggtta
      121 atgtgccggc tttaatcatt tctccaccaa ttaaaagctc ttctacacta agaacagctc
      181 cattacaatt agtgatatga tttaatcgtg taagctgagc tttgtagttt cctttaaagt
      241 gaccactcac aaataaaaac ttaaattccg ttacagaaga tggatagact ttccaccatt
      301 cattagggtt gatatgtttg tttcgtgttt gattttcttc gacatatcgt tgcatttcat
      361 ctgcttggcc aattggcaga ttataacctc cgctataagc tttagtatcc acgatcacac
      421 cgtaatcaat aggagatccg acagtataaa ttgctccgtc cggtttcctt gatccaccca
      481 aatgtttacc tctatatcca taaactttca taaaaaattc cattaccttc atttcaagaa
      541 ttctatcctg agtggaattt ctggcaattt caattaattc aatatattca tgaggcacat
      601 atttcaattt atgacgaagt tcagatttct tctcctccag ttcacttttg actagttggg
      661 atcccgcgac tcgatgggaa gttctctcgg gaatccgccg gttggttctt ttgatcaatg
      721 cgggagcatg aggcagaccc tttttgactg catcgagcgc gggtcgtcca ccaagacatg
      781 ccaacgccac cagatggtca ttcgttaacg cagccaacgc gggatcgggc ctggacaatt
      841 gggctacgat gg
//
