LOCUS       sequence-453295-        4719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0017h is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP20170 - pAAV-AiE0017h-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     regulatory      661..670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             673..693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1759..1870
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1982..2122
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1982..2111
                     /label=AAV2 ITR
     rep_origin      2197..2652
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2284..2303)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2494..2515
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2669..2688)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2788..2810
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2848..2866)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2934..3038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3039..3107,3108..3899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3257..3276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4070..4658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4559..4578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggtaa cagcctgagg gctgcaggtc
      181 acacccacag ggccaagggg tgggcagcat ggaggtgcag ggtggcaggg accctgggcg
      241 gggcggcaca gctgtgcggg aggctgggct gctggcatca gcaggcgccc ctcctcccca
      301 cctcgctaaa caatcattgc acaaaatatg caaatggtat aattactgtt atttgttttg
      361 ctgatataag tgtttgaaat gcaaatgtca agtttgggcg ccttcatttt tccaaccctc
      421 tcacccggac atttgcaagt tgatgagttg ttcttcatcc tggaaggagg aggaggagct
      481 ccccccaacc gccagggtgc caggggagtg agtccagcgt ggcagccgcc actgcctgcc
      541 cgagggcact gctgggcccc ccttccgacg gcacacagtg agctcgggct gggcataaaa
      601 gtcagggcag agccatctat tgcttacatt tgcttctggg atccagatct ttcgaagcta
      661 gccaccatgg tgcccaagaa gaagaggaaa gtctccaacc tgctgactgt gcaccaaaac
      721 ctgcctgccc tccctgtgga tgccacctct gatgaagtca ggaagaacct gatggacatg
      781 ttcagggaca ggcaggcctt ctctgaacac acctggaaga tgctcctgtc tgtgtgcaga
      841 tcctgggctg cctggtgcaa gctgaacaac aggaaatggt tccctgctga acctgaggat
      901 gtgagggact acctcctgta cctgcaagcc agaggcctgg ctgtgaagac catccaacag
      961 cacctgggcc agctcaacat gctgcacagg agatctggcc tgcctcgccc ttctgactcc
     1021 aatgctgtgt ccctggtgat gaggagaatc agaaaggaga atgtggatgc tggggagaga
     1081 gccaagcagg ccctggcctt tgaacgcact gactttgacc aagtcagatc cctgatggag
     1141 aactctgaca gatgccagga catcaggaac ctggccttcc tgggcattgc ctacaacacc
     1201 ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg acatctcccg caccgatggt
     1261 gggagaatgc tgatccacat tggcaggacc aagaccctgg tgtccacagc tggtgtggag
     1321 aaggccctgt ccctgggggt taccaagctg gtggagagat ggatctctgt gtctggtgtg
     1381 gctgatgacc ccaacaacta cctgttctgc cgggtcagaa agaatggtgt ggctgcccct
     1441 tctgccacct cccaactgtc cacccgggcc ctggaaggga tctttgaggc cacccaccgc
     1501 ctgatctatg gtgccaagga tgactctggg cagagatacc tggcctggtc tggccactct
     1561 gccagagtgg gtgctgccac cgacatggcc agggctggtg tgtccatccc tgaaatcatg
     1621 caggctggtg gctggaccaa tgtgaacatt gtgatgaact acatcagaaa cctggactct
     1681 gagactgggg ccatggtgag gctgctcgaa gatggggact gaggcgcgcc gaattcaagc
     1741 ttctcgagag atcttcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     1801 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     1861 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     1921 acagcaaggg ggaggattgg gaagacaata gcaggcatgc acgtgcggac cgagcggccg
     1981 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     2041 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     2101 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     2161 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     2221 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2281 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2341 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2401 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2461 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2521 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     2581 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2641 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2701 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     2761 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     2821 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     2881 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     2941 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3001 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3061 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3121 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3181 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3241 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3301 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3361 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3421 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3481 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3541 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3601 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3661 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3721 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3781 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3841 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3901 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3961 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4021 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4081 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4141 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4201 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4261 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4321 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4441 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4621 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4681 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
