LOCUS       sequence-453298-       17750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for 
            dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was 
            driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17750)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 17750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT13
FEATURES             Location/Qualifiers
     source          1..17750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..184
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        173..219
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             294..1319
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1360..1534
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1612..1636
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3260..4054
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4141..4729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4630..4649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4883..4900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4915..5055
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4981..5000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5141..5163
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5399..5593
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5659..6732)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7161..7790)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9091..9115
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9318..9334
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10017..10041
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10708..10773
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             10780..10800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(10825..10848,10849..10851,10852..14925)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             14838..14846
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             14926..14973
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             14995..15243
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             15256..15276
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      15298..15550
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    15595..15615
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(15976..16281)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGL"
     primer_bind     16146..16162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16172..16191
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16172..16190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(16238..16258)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16238..16256)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16277..16293)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    16301..16317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16325..16331,16332..16349,16350..16355))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16370..16391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        16433..16508
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(16711..16735)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      16799..17051
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(17068..17084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    17092..17108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(17116..17122,17123..17140,17141..17146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17161..17182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
       61 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      121 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
      181 cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc
      241 tctctacaaa tctatctctc tcgagctttc gcagatccgg ggggcaatga gatatgaaaa
      301 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
      361 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      421 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      481 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt
      541 ttagcgagag cctgacctat tgcatctccc gccgttcaca gggtgtcacg ttgcaagacc
      601 tgcctgaaac cgaactgccc gctgttctac aaccggtcgc ggaggctatg gatgcgatcg
      661 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
      721 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
      781 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
      841 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
      901 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
      961 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     1021 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccacgactcc
     1081 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     1141 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     1201 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     1261 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aagaaataga
     1321 gtagatgccg accgggatct gtcgatcgac aagctcgagt ttctccataa taatgtgtga
     1381 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
     1441 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     1501 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
     1561 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     1621 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     1681 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     1741 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     1801 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     1861 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
     1921 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
     1981 agtgaattgg agttcgtctt gttataatta gggaaggtgc gaacaagtcc ctgatatgag
     2041 atcatgtttg tcatctggag ccatagaaca gggttcatca tgagtcatca acttaccttc
     2101 gccgacagtg aattcagcag taagcgccgt cagaccagaa aagagatttt cttgtcccgc
     2161 atggagcaga ttctgccatg gcaaaacatg gtggaagtca tcgagccgtt ttaccccaag
     2221 gctggtaatg gccggcgacc ttatccgctg gaaaccatgc tacgcattca ctgcatgcag
     2281 cattggtaca acctgagcga tggcgcgatg gaagatgctc tgtacgaaat cgcctccatg
     2341 cgtctgtttg cccggttatc cctggatagc gccttgccgg accgcaccac catcatgaat
     2401 ttccgccacc tgctggagca gcatcaactg gcccgccaat tgttcaagac catcaatcgc
     2461 tggctggccg aagcaggcgt catgatgact caaggcacct tggtcgatgc caccatcatt
     2521 gaggcaccca gctcgaccaa gaacaaagag cagcaacgcg atccggagat gcatcagacc
     2581 aagaaaggca atcagtggca ctttggcatg aaggcccaca ttggtgtcga tgccaagagt
     2641 ggcctgaccc acagcctggt caccaccgcg gccaacgagc atgacctcaa tcagctgggt
     2701 aatctgctgc atggagagga gcaatttgtc tcagccgatg ccggctacca aggggcgcca
     2761 cagcgcgagg agctggccga ggtggatgtg gactggctga tcgccgagcg ccccggcaag
     2821 gtaagaacct tgaaacagca tccacgcaag aacaaaacgg ccatcaacat cgaatacatg
     2881 aaagccagca tccgggccag ggtggagcac ccatttcgca tcatcaagcg acagttcggc
     2941 ttcgtgaaag ccagatacaa ggggttgctg aaaaacgata accaactggc gatgttattc
     3001 acgctggcca acctgtttcg ggcggaccaa atgatacgtc agtgggagag atctcactaa
     3061 aaactgggga taacgcctta aatggcgaag aaacggtcta aataggctga ttcaaggcat
     3121 ttacgggaga aaaaatcggc tcaaacatga agaaatgaaa tgactgagtc agccgagaag
     3181 aatttccccg cttattcgca ccttccttag cttcttgggg tatctttaaa tactgtagaa
     3241 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
     3301 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     3361 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     3421 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     3481 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     3541 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     3601 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     3661 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
     3721 ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt
     3781 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
     3841 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
     3901 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga
     3961 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
     4021 aaaatattat attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc
     4081 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4141 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4201 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4261 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4321 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4381 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4441 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4501 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4561 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4621 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4681 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4741 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4801 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4861 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     4921 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     4981 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     5041 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5101 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5161 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     5221 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     5281 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     5341 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     5401 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     5461 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     5521 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     5581 cgtgctatcc acaggaaaca gaccttttcg acctttttcc cctgctaggg caatttgccc
     5641 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     5701 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     5761 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     5821 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     5881 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     5941 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     6001 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     6061 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     6121 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     6181 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     6241 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     6301 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     6361 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     6421 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     6481 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     6541 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     6601 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     6661 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     6721 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     6781 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     6841 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     6901 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     6961 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     7021 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     7081 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     7141 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     7201 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     7261 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     7321 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     7381 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     7441 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     7501 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     7561 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     7621 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     7681 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     7741 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     7801 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     7861 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     7921 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     7981 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     8041 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     8101 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     8161 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     8221 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     8281 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     8341 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     8401 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     8461 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     8521 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     8581 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     8641 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     8701 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     8761 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     8821 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     8881 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     8941 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     9001 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     9061 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     9121 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     9181 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     9241 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     9301 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctttcgacga gtcagtaata
     9361 aacggcgtca aagtggttgc agccggcaca cacgagtcgt gtttatcaac tcaaagcaca
     9421 aatacttttc ctcaacctaa aaataaggca attagccaaa aacaactttg cgtgtaaaca
     9481 acgctcaata cacgtgtcat tttattatta gctattgctt caccgcctta gctttctcgt
     9541 gacctagtcg tcctcgtctt ttcttcttct tcttctataa aacaataccc aaagagctct
     9601 tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc tcaattctct
     9661 ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc gattttgttt
     9721 tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc ttagatcgaa
     9781 gacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg gtttcataga
     9841 tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt cgatttgtga
     9901 tttctatcta gatctggtgt tagtttctag tttgtgcgat cgaatttgta gattaatctg
     9961 agtttttctg attaacaggg taccgggccc cccctcgagg cgcgccaagc tatcaaacaa
    10021 gtttgtacaa aaaagcaggc tccgaattcg cccttcatgg aggcctctcc ggcttcaggt
    10081 cctcgccatc tcatggaccc ccatatcttt acctccaatt tcaataacgg cattggcagg
    10141 cacaagacat acctctgtta tgaggtcgag cggctcgata acggaacctc agtgaagatg
    10201 gaccaacaca ggggtttcct tcataaccaa gcgaaaaatc tcctttgcgg tttctatggg
    10261 cggcacgcgg agctgagatt cttggatttg gtcccgagcc tccagctgga tcccgcgcaa
    10321 atataccgcg tcacttggtt catatcatgg agcccgtgct ttagctgggg gtgtgccggt
    10381 gaggttcgcg cgttcctcca ggaaaacacc catgttcgcc tccggatttt cgcagcgagg
    10441 attttcgact atgatccact ttacaaagag gccctccaaa tgcttagaga tgctggggct
    10501 caagtttcaa ttatgacata cgacgagttt aagcattgtt gggacacttt tgtcgatcat
    10561 cagggttgtc ccttccagcc gtgggatggt cttgatgagc attcgcaagc tttgagtggc
    10621 aggcttcgcg cgatacttca gaaccagggt aactcaggtt ctgagacgcc tggcacaagt
    10681 gagtcagcaa cacccgagtc caccatggat tacaaggacc acgacgggga ttacaaggac
    10741 cacgacattg attacaagga tgatgatgac aagatggctc cgaagaagaa gaggaaggtt
    10801 ggcatccacg gggtgccagc tgctgacaag aagtactcga tcggcctcgc tattgggact
    10861 aactctgttg gctgggccgt gatcaccgac gagtacaagg tgccctcaaa gaagttcaag
    10921 gtcctgggca acaccgatcg gcattccatc aagaagaatc tcattggcgc tctcctgttc
    10981 gacagcggcg agacggctga ggctacgcgg ctcaagcgca ccgcccgcag gcggtacacg
    11041 cgcaggaaga atcgcatctg ctacctgcag gagattttct ccaacgagat ggcgaaggtt
    11101 gacgattctt tcttccacag gctggaggag tcattcctcg tggaggagga taagaagcac
    11161 gagcggcatc caatcttcgg caacattgtc gacgaggttg cctaccacga gaagtaccct
    11221 acgatctacc atctgcggaa gaagctcgtg gactccacag ataaggcgga cctccgcctg
    11281 atctacctcg ctctggccca catgattaag ttcaggggcc atttcctgat cgagggggat
    11341 ctcaacccgg acaatagcga tgttgacaag ctgttcatcc agctcgtgca gacgtacaac
    11401 cagctcttcg aggagaaccc cattaatgcg tcaggcgtcg acgcgaaggc tatcctgtcc
    11461 gctaggctct cgaagtctcg gcgcctcgag aacctgatcg cccagctgcc gggcgagaag
    11521 aagaacggcc tgttcgggaa tctcattgcg ctcagcctgg ggctcacgcc caacttcaag
    11581 tcgaatttcg atctcgctga ggacgccaag ctgcagctct ccaaggacac atacgacgat
    11641 gacctggata acctcctggc ccagatcggc gatcagtacg cggacctgtt cctcgctgcc
    11701 aagaatctgt cggacgccat cctcctgtct gatattctca gggtgaacac cgagattacg
    11761 aaggctccgc tctcagcctc catgatcaag cgctacgacg agcaccatca ggatctgacc
    11821 ctcctgaagg cgctggtcag gcagcagctc cccgagaagt acaaggagat cttcttcgat
    11881 cagtcgaaga acggctacgc tgggtacatt gacggcgggg cctctcagga ggagttctac
    11941 aagttcatca agccgattct ggagaagatg gacggcacgg aggagctgct ggtgaagctc
    12001 aatcgcgagg acctcctgag gaagcagcgg acattcgata acggcagcat cccacaccag
    12061 attcatctcg gggagctgca cgctatcctg aggaggcagg aggacttcta ccctttcctc
    12121 aaggataacc gcgagaagat cgagaagatt ctgactttca ggatcccgta ctacgtcggc
    12181 ccactcgcta ggggcaactc ccgcttcgct tggatgaccc gcaagtcaga ggagacgatc
    12241 acgccgtgga acttcgagga ggtggtcgac aagggcgcta gcgctcagtc gttcatcgag
    12301 aggatgacga atttcgacaa gaacctgcca aatgagaagg tgctccctaa gcactcgctc
    12361 ctgtacgagt acttcacagt ctacaacgag ctgactaagg tgaagtatgt gaccgagggc
    12421 atgaggaagc cggctttcct gtctggggag cagaagaagg ccatcgtgga cctcctgttc
    12481 aagaccaacc ggaaggtcac ggttaagcag ctcaaggagg actacttcaa gaagattgag
    12541 tgcttcgatt cggtcgagat ctctggcgtt gaggaccgct tcaacgcctc cctggggacc
    12601 taccacgatc tcctgaagat cattaaggat aaggacttcc tggacaacga ggagaatgag
    12661 gatatcctcg aggacattgt gctgacactc actctgttcg aggaccggga gatgatcgag
    12721 gagcgcctga agacttacgc ccatctcttc gatgacaagg tcatgaagca gctcaagagg
    12781 aggaggtaca ccggctgggg gaggctgagc aggaagctca tcaacggcat tcgggacaag
    12841 cagtccggga agacgatcct cgacttcctg aagagcgatg gcttcgcgaa ccgcaatttc
    12901 atgcagctga ttcacgatga cagcctcaca ttcaaggagg atatccagaa ggctcaggtg
    12961 agcggccagg gggactcgct gcacgagcat atcgcgaacc tcgctggctc gccagctatc
    13021 aagaagggga ttctgcagac cgtgaaggtt gtggacgagc tggtgaaggt catgggcagg
    13081 cacaagcctg agaacatcgt cattgagatg gcccgggaga atcagaccac gcagaagggc
    13141 cagaagaact cacgcgagag gatgaagagg atcgaggagg gcattaagga gctggggtcc
    13201 cagatcctca aggagcaccc ggtggagaac acgcagctgc agaatgagaa gctctacctg
    13261 tactacctcc agaatggccg cgatatgtat gtggaccagg agctggatat taacaggctc
    13321 agcgattacg acgtcgatca tatcgttcca cagtcattcc tgaaggatga ctccattgac
    13381 aacaaggtcc tcaccaggtc ggacaagaac cggggcaagt ctgataatgt tccttcagag
    13441 gaggtcgtta agaagatgaa gaactactgg cgccagctcc tgaatgccaa gctgatcacg
    13501 cagcggaagt tcgataacct cacaaaggct gagaggggcg ggctctctga gctggacaag
    13561 gcgggcttca tcaagaggca gctggtcgag acacggcaga tcactaagca cgttgcgcag
    13621 attctcgact cacggatgaa cactaagtac gatgagaatg acaagctgat ccgcgaggtg
    13681 aaggtcatca ccctgaagtc aaagctcgtc tccgacttca ggaaggattt ccagttctac
    13741 aaggttcggg agatcaacaa ttaccaccat gcccatgacg cgtacctgaa cgcggtggtc
    13801 ggcacagctc tgatcaagaa gtacccaaag ctcgagagcg agttcgtgta cggggactac
    13861 aaggtttacg atgtgaggaa gatgatcgcc aagtcggagc aggagattgg caaggctacc
    13921 gccaagtact tcttctactc taacattatg aatttcttca agacagagat cactctggcc
    13981 aatggcgaga tccggaagcg ccccctcatc gagacgaacg gcgagacggg ggagatcgtg
    14041 tgggacaagg gcagggattt cgcgaccgtc aggaaggttc tctccatgcc acaagtgaat
    14101 atcgtcaaga agacagaggt ccagactggc gggttctcta aggagtcaat tctgcctaag
    14161 cggaacagcg acaagctcat cgcccgcaag aaggactggg atccgaagaa gtacggcggg
    14221 ttcgacagcc ccactgtggc ctactcggtc ctggttgtgg cgaaggttga gaagggcaag
    14281 tccaagaagc tcaagagcgt gaaggagctg ctggggatca cgattatgga gcgctccagc
    14341 ttcgagaaga acccgatcga tttcctggag gcgaagggct acaaggaggt gaagaaggac
    14401 ctgatcatta agctccccaa gtactcactc ttcgagctgg agaacggcag gaagcggatg
    14461 ctggcttccg ctggcgagct gcagaagggg aacgagctgg ctctgccgtc caagtatgtg
    14521 aacttcctct acctggcctc ccactacgag aagctcaagg gcagccccga ggacaacgag
    14581 cagaagcagc tgttcgtcga gcagcacaag cattacctcg acgagatcat tgagcagatt
    14641 tccgagttct ccaagcgcgt gatcctggcc gacgcgaatc tggataaggt cctctccgcg
    14701 tacaacaagc accgcgacaa gccaatcagg gagcaggctg agaatatcat tcatctcttc
    14761 accctgacga acctcggcgc ccctgctgct ttcaagtact tcgacacaac tatcgatcgc
    14821 aagaggtaca caagcactaa ggaggtcctg gacgcgaccc tcatccacca gtcgattacc
    14881 ggcctctacg agacgcgcat cgacctgtct cagctcgggg gcgacaagcg gccagcggcg
    14941 acgaagaagg cggggcaggc gaagaagaag aagggagacg gatcaggcgg gtcaacaaac
    15001 ctcagtgaca tcatagagaa ggaaactggt aagcaactgg ttattcaaga gagcattctg
    15061 atgctccctg aggaagtcga ggaagttata ggaaacaagc ctgagagcga tatactcgtg
    15121 catacggcgt atgacgagag cacggatgaa aatgtgatgc tcttgaccag cgatgcgcca
    15181 gaatataaac cctgggcact ggttattcag gactctaacg gggaaaataa aatcaagatg
    15241 ctctcaggtg gctccccaaa gaagaaacgc aaggtttagt ggcggccgcc gacgtccgat
    15301 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
    15361 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
    15421 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
    15481 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
    15541 ttactagatc gggaattgat cccccctcga cagcttccgg aaagggcgaa ttcgcaactt
    15601 tgtatacaaa agttgcccca tggcgttccc tctagataac gcaggatcct atttacttta
    15661 aatttttctt atggctcagc ctgtgatgga taactgaatc aaacaaatgg cgtctgggtt
    15721 taagaacatc tgttttggct atgttggacg aaacaagtga acttttagga tcaacttccg
    15781 tttatatacg gagcttatat cgagcaataa gataagtggg ctttttatgt aatttaatgg
    15841 gctatcgtcc atatattcac taatacccat gcccagtacc catgtatgcg tttcatataa
    15901 gctcctaatt tctcccacat cgctcaaatc taaacaaatc ttgttgtata tataacactg
    15961 agggagcacc attggtcaga gaccggcgcc gctacagggc gcgtcccatt cgccattcag
    16021 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    16081 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    16141 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
    16201 taccgggccc cccctcgagg tcctccagct tttgttccct ttagtgaggg ttaattgcgc
    16261 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    16321 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    16381 aactcacatt aattgcgttg cgctcactgc ccgctttcca ccggtggtct ctgttttaga
    16441 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
    16501 gtcggtgctt ttttttccct ttcctttttt cttttttttg ccataaactt aaatttgtat
    16561 atcgatcatt gtagatattg aaaacctaga acaaaccaac atccatgtga atgtctttca
    16621 tgactgattt agagataatt cttgaatttt ggaactagaa tctataatga gcctaaatta
    16681 aaacattgtg aactagtaag ggcgaattcg acccagcttt cttgtacaaa gtggttcgat
    16741 aattcttaat taactagttc tagagcggcc gccaccgcgg tggagctcga atttccccga
    16801 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
    16861 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
    16921 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
    16981 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
    17041 gttactagat cgggaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
    17101 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    17161 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    17221 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    17281 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc
    17341 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
    17401 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
    17461 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
    17521 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
    17581 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
    17641 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
    17701 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
//
