LOCUS       sequence-453299-       18461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG
            PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 
            promoter; Hygromycin for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18461)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 18461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT04
FEATURES             Location/Qualifiers
     source          1..18461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..184
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        173..219
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             294..1319
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1360..1534
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1612..1636
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3260..4054
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4141..4729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4630..4649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4883..4900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4915..5055
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4981..5000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5141..5163
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5399..5593
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5659..6732)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7161..7790)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9085..9109
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9312..9328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9344..11340
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    11383..11407
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11429..11494
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11501..11521
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11546..15646
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             11546..15646
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             15559..15567
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             15647..15694
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    16369..16389
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        16426..16803
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             complement(16774..17106)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLCHG
                     SSAQLF"
     primer_bind     16971..16987
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16997..17016
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16997..17015
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(17063..17083)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(17063..17081)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(17102..17118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    17126..17142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(17150..17156,17157..17174,17175..17180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17195..17216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        17258..17333
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(17422..17446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      17510..17762
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(17779..17795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    17803..17819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(17827..17833,17834..17851,17852..17857))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17872..17893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
       61 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      121 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
      181 cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc
      241 tctctacaaa tctatctctc tcgagctttc gcagatccgg ggggcaatga gatatgaaaa
      301 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
      361 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      421 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      481 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt
      541 ttagcgagag cctgacctat tgcatctccc gccgttcaca gggtgtcacg ttgcaagacc
      601 tgcctgaaac cgaactgccc gctgttctac aaccggtcgc ggaggctatg gatgcgatcg
      661 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
      721 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
      781 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
      841 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
      901 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
      961 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     1021 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccacgactcc
     1081 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     1141 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     1201 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     1261 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aagaaataga
     1321 gtagatgccg accgggatct gtcgatcgac aagctcgagt ttctccataa taatgtgtga
     1381 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
     1441 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     1501 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
     1561 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     1621 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     1681 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     1741 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     1801 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     1861 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
     1921 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
     1981 agtgaattgg agttcgtctt gttataatta gggaaggtgc gaacaagtcc ctgatatgag
     2041 atcatgtttg tcatctggag ccatagaaca gggttcatca tgagtcatca acttaccttc
     2101 gccgacagtg aattcagcag taagcgccgt cagaccagaa aagagatttt cttgtcccgc
     2161 atggagcaga ttctgccatg gcaaaacatg gtggaagtca tcgagccgtt ttaccccaag
     2221 gctggtaatg gccggcgacc ttatccgctg gaaaccatgc tacgcattca ctgcatgcag
     2281 cattggtaca acctgagcga tggcgcgatg gaagatgctc tgtacgaaat cgcctccatg
     2341 cgtctgtttg cccggttatc cctggatagc gccttgccgg accgcaccac catcatgaat
     2401 ttccgccacc tgctggagca gcatcaactg gcccgccaat tgttcaagac catcaatcgc
     2461 tggctggccg aagcaggcgt catgatgact caaggcacct tggtcgatgc caccatcatt
     2521 gaggcaccca gctcgaccaa gaacaaagag cagcaacgcg atccggagat gcatcagacc
     2581 aagaaaggca atcagtggca ctttggcatg aaggcccaca ttggtgtcga tgccaagagt
     2641 ggcctgaccc acagcctggt caccaccgcg gccaacgagc atgacctcaa tcagctgggt
     2701 aatctgctgc atggagagga gcaatttgtc tcagccgatg ccggctacca aggggcgcca
     2761 cagcgcgagg agctggccga ggtggatgtg gactggctga tcgccgagcg ccccggcaag
     2821 gtaagaacct tgaaacagca tccacgcaag aacaaaacgg ccatcaacat cgaatacatg
     2881 aaagccagca tccgggccag ggtggagcac ccatttcgca tcatcaagcg acagttcggc
     2941 ttcgtgaaag ccagatacaa ggggttgctg aaaaacgata accaactggc gatgttattc
     3001 acgctggcca acctgtttcg ggcggaccaa atgatacgtc agtgggagag atctcactaa
     3061 aaactgggga taacgcctta aatggcgaag aaacggtcta aataggctga ttcaaggcat
     3121 ttacgggaga aaaaatcggc tcaaacatga agaaatgaaa tgactgagtc agccgagaag
     3181 aatttccccg cttattcgca ccttccttag cttcttgggg tatctttaaa tactgtagaa
     3241 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
     3301 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     3361 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     3421 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     3481 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     3541 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     3601 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     3661 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
     3721 ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt
     3781 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
     3841 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
     3901 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga
     3961 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
     4021 aaaatattat attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc
     4081 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4141 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4201 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4261 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4321 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4381 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4441 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4501 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4561 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4621 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4681 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4741 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4801 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4861 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     4921 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     4981 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     5041 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5101 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5161 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     5221 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     5281 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     5341 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     5401 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     5461 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     5521 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     5581 cgtgctatcc acaggaaaca gaccttttcg acctttttcc cctgctaggg caatttgccc
     5641 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     5701 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     5761 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     5821 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     5881 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     5941 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     6001 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     6061 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     6121 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     6181 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     6241 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     6301 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     6361 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     6421 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     6481 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     6541 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     6601 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     6661 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     6721 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     6781 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     6841 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     6901 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     6961 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     7021 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     7081 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     7141 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     7201 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     7261 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     7321 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     7381 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     7441 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     7501 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     7561 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     7621 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     7681 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     7741 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     7801 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     7861 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     7921 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     7981 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     8041 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     8101 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctaggc
     8161 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
     8221 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
     8281 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
     8341 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
     8401 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
     8461 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
     8521 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
     8581 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
     8641 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
     8701 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
     8761 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
     8821 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
     8881 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
     8941 atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca
     9001 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
     9061 ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag
     9121 tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctgctct agcattcgcc
     9181 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     9241 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     9301 gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcctgcagt gcagcgtgac
     9361 ccggtcgtgc ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc
     9421 acatattttt tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt
     9481 aaactttact ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga
     9541 atcatataaa tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga
     9601 ctctacagtt ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca
     9661 cctatataat acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt
     9721 tttatagact aattttttta gtacatctat tttattctat tttagcctct aaattaagaa
     9781 aactaaaact ctattttagt ttttttattt aataatttag atataaaata gaataaaata
     9841 aagtgactaa aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt
     9901 tcttgtttcg agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc
     9961 aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt
    10021 cgctgcctct ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg
    10081 catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc
    10141 tcctctcacg gcaccggcag ctacggggga ttcctttccc accgctcctt cgctttccct
    10201 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
    10261 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
    10321 ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt
    10381 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
    10441 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
    10501 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
    10561 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
    10621 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
    10681 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
    10741 aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata
    10801 catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac
    10861 atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga
    10921 tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca
    10981 aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt
    11041 tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt
    11101 ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt
    11161 acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga
    11221 tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat acgctattta
    11281 tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag
    11341 gtcgactcta gaggatcccc tcgaggcgcg ccaagctatc aaacaagttt gtacaaaaaa
    11401 gcaggctccg aattcgccct tcaccatgga ttacaaggac cacgacgggg attacaagga
    11461 ccacgacatt gattacaagg atgatgatga caagatggct ccgaagaaga agaggaaggt
    11521 tggcatccac ggggtgccag ctgctgacaa gaagtactcg atcggcctcg atattgggac
    11581 taactctgtt ggctgggccg tgatcaccga cgagtacaag gtgccctcaa agaagttcaa
    11641 ggtcctgggc aacaccgatc ggcattccat caagaagaat ctcattggcg ctctcctgtt
    11701 cgacagcggc gagacggctg aggctacgcg gctcaagcgc accgcccgca ggcggtacac
    11761 gcgcaggaag aatcgcatct gctacctgca ggagattttc tccaacgaga tggcgaaggt
    11821 tgacgattct ttcttccaca ggctggagga gtcattcctc gtggaggagg ataagaagca
    11881 cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg agaagtaccc
    11941 tacgatctac catctgcgga agaagctcgt ggactccaca gataaggcgg acctccgcct
    12001 gatctacctc gctctggccc acatgattaa gttcaggggc catttcctga tcgaggggga
    12061 tctcaacccg gacaatagcg atgttgacaa gctgttcatc cagctcgtgc agacgtacaa
    12121 ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc
    12181 cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc cgggcgagaa
    12241 gaagaacggc ctgttcggga atctcattgc gctcagcctg gggctcacgc ccaacttcaa
    12301 gtcgaatttc gatctcgctg aggacgccaa gctgcagctc tccaaggaca catacgacga
    12361 tgacctggat aacctcctgg cccagatcgg cgatcagtac gcggacctgt tcctcgctgc
    12421 caagaatctg tcggacgcca tcctcctgtc tgatattctc agggtgaaca ccgagattac
    12481 gaaggctccg ctctcagcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac
    12541 cctcctgaag gcgctggtca ggcagcagct ccccgagaag tacaaggaga tcttcttcga
    12601 tcagtcgaag aacggctacg ctgggtacat tgacggcggg gcctctcagg aggagttcta
    12661 caagttcatc aagccgattc tggagaagat ggacggcacg gaggagctgc tggtgaagct
    12721 caatcgcgag gacctcctga ggaagcagcg gacattcgat aacggcagca tcccacacca
    12781 gattcatctc ggggagctgc acgctatcct gaggaggcag gaggacttct accctttcct
    12841 caaggataac cgcgagaaga tcgagaagat tctgactttc aggatcccgt actacgtcgg
    12901 cccactcgct aggggcaact cccgcttcgc ttggatgacc cgcaagtcag aggagacgat
    12961 cacgccgtgg aacttcgagg aggtggtcga caagggcgct agcgctcagt cgttcatcga
    13021 gaggatgacg aatttcgaca agaacctgcc aaatgagaag gtgctcccta agcactcgct
    13081 cctgtacgag tacttcacag tctacaacga gctgactaag gtgaagtatg tgaccgaggg
    13141 catgaggaag ccggctttcc tgtctgggga gcagaagaag gccatcgtgg acctcctgtt
    13201 caagaccaac cggaaggtca cggttaagca gctcaaggag gactacttca agaagattga
    13261 gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct ccctggggac
    13321 ctaccacgat ctcctgaaga tcattaagga taaggacttc ctggacaacg aggagaatga
    13381 ggatatcctc gaggacattg tgctgacact cactctgttc gaggaccggg agatgatcga
    13441 ggagcgcctg aagacttacg cccatctctt cgatgacaag gtcatgaagc agctcaagag
    13501 gaggaggtac accggctggg ggaggctgag caggaagctc atcaacggca ttcgggacaa
    13561 gcagtccggg aagacgatcc tcgacttcct gaagagcgat ggcttcgcga accgcaattt
    13621 catgcagctg attcacgatg acagcctcac attcaaggag gatatccaga aggctcaggt
    13681 gagcggccag ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cgccagctat
    13741 caagaagggg attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag
    13801 gcacaagcct gagaacatcg tcattgagat ggcccgggag aatcagacca cgcagaaggg
    13861 ccagaagaac tcacgcgaga ggatgaagag gatcgaggag ggcattaagg agctggggtc
    13921 ccagatcctc aaggagcacc cggtggagaa cacgcagctg cagaatgaga agctctacct
    13981 gtactacctc cagaatggcc gcgatatgta tgtggaccag gagctggata ttaacaggct
    14041 cagcgattac gacgtcgatc atatcgttcc acagtcattc ctgaaggatg actccattga
    14101 caacaaggtc ctcaccaggt cggacaagaa ccggggcaag tctgataatg ttccttcaga
    14161 ggaggtcgtt aagaagatga agaactactg gcgccagctc ctgaatgcca agctgatcac
    14221 gcagcggaag ttcgataacc tcacaaaggc tgagaggggc gggctctctg agctggacaa
    14281 ggcgggcttc atcaagaggc agctggtcga gacacggcag atcactaagc acgttgcgca
    14341 gattctcgac tcacggatga acactaagta cgatgagaat gacaagctga tccgcgaggt
    14401 gaaggtcatc accctgaagt caaagctcgt ctccgacttc aggaaggatt tccagttcta
    14461 caaggttcgg gagatcaaca attaccacca tgcccatgac gcgtacctga acgcggtggt
    14521 cggcacagct ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt acggggacta
    14581 caaggtttac gatgtgagga agatgatcgc caagtcggag caggagattg gcaaggctac
    14641 cgccaagtac ttcttctact ctaacattat gaatttcttc aagacagaga tcactctggc
    14701 caatggcgag atccggaagc gccccctcat cgagacgaac ggcgagacgg gggagatcgt
    14761 gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa
    14821 tatcgtcaag aagacagagg tccagactgg cgggttctct aaggagtcaa ttctgcctaa
    14881 gcggaacagc gacaagctca tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg
    14941 gttcgacagc cccactgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa
    15001 gtccaagaag ctcaagagcg tgaaggagct gctggggatc acgattatgg agcgctccag
    15061 cttcgagaag aacccgatcg atttcctgga ggcgaagggc tacaaggagg tgaagaagga
    15121 cctgatcatt aagctcccca agtactcact cttcgagctg gagaacggca ggaagcggat
    15181 gctggcttcc gctggcgagc tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt
    15241 gaacttcctc tacctggcct cccactacga gaagctcaag ggcagccccg aggacaacga
    15301 gcagaagcag ctgttcgtcg agcagcacaa gcattacctc gacgagatca ttgagcagat
    15361 ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat ctggataagg tcctctccgc
    15421 gtacaacaag caccgcgaca agccaatcag ggagcaggct gagaatatca ttcatctctt
    15481 caccctgacg aacctcggcg cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg
    15541 caagaggtac acaagcacta aggaggtcct ggacgcgacc ctcatccacc agtcgattac
    15601 cggcctctac gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc ggccagcggc
    15661 gacgaagaag gcggggcagg cgaagaagaa gaagtgagct cagagctttc gttcgtatca
    15721 tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcgcac acaccagaat
    15781 cctactgagt ttgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg
    15841 cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga
    15901 tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt
    15961 tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt
    16021 tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa
    16081 acacttgtag ttgtaccatt atgcttattc actaggcaac aaatatattt tcagacctag
    16141 aaaagctgca aatgttactg aatacaagta tgtcctcttg tgttttagac atttatgaac
    16201 tttcctttat gtaattttcc agaatccttg tcagattcta atcattgctt tataattata
    16261 gttatactca tggatttgta gttgagtatg aaaatatttt ttaatgcatt ttatgacttg
    16321 ccaattgttc cggaactcta gataagctta ccggaaaggg cgaattcgca actttgtata
    16381 caaaagttgc cccatggcgt tccctctaga taacgcagga tcctaaaggg atctttaaac
    16441 atacgaacag atcacttaaa gttcttctga agcaacttaa agttatcagg catgcatgga
    16501 tcttggagga atcagatgtg cagtcaggga ccatagcaca ggacaggcgt cttctactgg
    16561 tgctaccagc aaatgctgga agccgggaac actgggtacg ttggaaacca cgtgatgtgg
    16621 agtaagataa actgtaggag aaaagcattt cgtagtgggc catgaagcct ttcaggacat
    16681 gtattgcagt atgggccggc ccattacgca attggacgac aacaaagact agtattagta
    16741 ccacctcggc tatccacata gatcaaagct ggtttaaaag agttgtgcag atgatccgtg
    16801 gcagagaccg gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
    16861 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    16921 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    16981 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct
    17041 cgaggtcctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
    17101 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    17161 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    17221 cgttgcgctc actgcccgct ttccaccggt ggtctctgtt ttagagctag aaatagcaag
    17281 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
    17341 gtcccttcga agggcaattc tgcagatatc catcacactg gcggccgctc gaggtcgagg
    17401 gtatcgataa gcttgaattc gacccagctt tcttgtacaa agtggttcga taattcttaa
    17461 ttaactagtt ctagagcggc cgccaccgcg gtggagctcg aatttccccg atcgttcaaa
    17521 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
    17581 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
    17641 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
    17701 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
    17761 tcgggaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    17821 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    17881 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    17941 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag
    18001 cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
    18061 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
    18121 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
    18181 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
    18241 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
    18301 ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg acactctcgt
    18361 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
    18421 acaaagggta atatcgggaa acctcctcgg attccattgc c
//
