LOCUS       sequence-45335-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALETF_v2 (NN)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             717..737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             762..911
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacgg tctcgactca
      121 cgcctgagca ggtagtggct attgcatcca ataacggggg cagacccgca ctggagtcaa
      181 tcgtggccca gctttcgagg ccggaccccg cgctggccgc actcactaat gatcatcttg
      241 tagcgctggc ctgcctcggc ggacgacccg ccttggatgc ggtgaagaag gggctcccgc
      301 acgcgcctgc attgattaag cggaccaaca gaaggattcc cgagaggaca tcacatcgag
      361 tggcagatca cgcgcaagtg gtccgcgtgc tcggattctt ccagtgtcac tcccaccccg
      421 cacaagcgtt cgatgacgcc atgactcaat ttggtatgtc gagacacgga ctgctgcagc
      481 tctttcgtag agtcggtgtc acagaactcg aggcccgctc gggcacactg cctcccgcct
      541 cccagcggtg ggacaggatt ctccaagcga gcggtatgaa acgcgcgaag ccttcaccta
      601 cgtcaactca gacacctgac caggcgagcc ttcatgcgtt cgcagactcg ctggagaggg
      661 atttggacgc gccctcgccc atgcatgaag gggaccaaac tcgcgcgtca gctagcccca
      721 agaagaagag aaaggtggag gccagcggtt ccggacgggc tgacgcattg gacgattttg
      781 atctggatat gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg
      841 atgcccttga tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc
      901 tggacatgct ga
//
