LOCUS       sequence-453370-        5426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0780m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in striatal direct pathway MSNs (D1-MSNs).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP15582: pAAV-AiE0780m-minBG-jRGECO1a-WPRE3-B...
FEATURES             Location/Qualifiers
     source          1..5426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     RBS             375..383
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             831..848
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             852..884
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(888..896,897..911)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     1691..1710
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2253..2273)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2460..2571
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2689..2829
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2689..2818
                     /label=AAV2 ITR
     rep_origin      2904..3359
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2991..3010)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3201..3222
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3376..3395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3495..3517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3555..3573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3641..3745
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3746..3814,3815..4606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3964..3983)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4777..5365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5266..5285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttgttt cacaaactac aggttcactg
      181 gagcacagcc atgccaacat ggcatctaag gctggtatga cctcgagacc cctccagcac
      241 tccttcgttc agccatagaa caaggagaca cagataaagt ctggagccgg gcccaggtac
      301 ctgaggctca gagatgagcc cgcagcgaag ctgacaactg actcattcct ccgacactct
      361 cccaccctcc cagctaagga ggtgtctgat gtgaacatgt tctgcctctt tgttgcctgc
      421 tgttgggggc gagggctttg ggtggatttt gaagagcaat tatgcttatt actggttagg
      481 atttctctgt ttgctgagtg cattaacttt tagcgcttcc catcactcat tcttcttcac
      541 accctcttgt cagttacaaa tgtgcaggtg gagagccacg aaaaaaccct gcagcaacgg
      601 caacagaaat cacagcaaca acaacaaatg aacagaaagg atagaaagca gatactcaaa
      661 gatggagaaa aagatactcc cgagaggctg agctcgggct gggcataaaa gtcagggcag
      721 agccatctat tgcttacatt tgcttctggg atccacccgc caccatgctg cagaacgagc
      781 ttgctcttaa gttggctgga cttgatatta acaagactgg aggaggttct catcatcatc
      841 atcatcatgg tatggcaagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg
      901 atgacgataa ggatctcgca acaatggtcg actcatcacg tcgtaagtgg aataaggcag
      961 gtcacgcagt cagagctata ggtcggctga gctcccccgt ggtttccgag cggatgtacc
     1021 ccgaggacgg cgccctgaag agcgagatca agaaggggct gaggctgaag gacggcggcc
     1081 actacgccgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     1141 cctacatcgt ggacatcaag ttggacatcg tgtcccacaa cgaggactac accatcgtgg
     1201 aacagtgcga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
     1261 gaggtacagg cgggagtctg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     1321 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     1381 agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg aaggtgacca
     1441 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     1501 aggcctacat taagcaccca gccgacatcc ccgactactt caagctgtcc ttccccgagg
     1561 gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac gtgaaccagg
     1621 actcctccct gcaggacggc gtattcatct acaaggtgaa gctgcgcggc accaacttcc
     1681 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggctact cgtgacgacc
     1741 tgactgaaga gcagatcgca gaatttaaag aggctttctc cctatttgac aaggacgggg
     1801 atgggacgat aacaaccaag gagcttggga cggtgttccg atcgctgggg cagaacccca
     1861 cagaagcaga gctgcaggac atgatcaatg aagtagatgc cgacggtgac ggcacattcg
     1921 acttccctga gttcctgacg atgatggcaa gaaaaatgaa tgacacagac agtgaagagg
     1981 aaattcgcga agcgttccgc gtgtttgata aggacggcaa tggctacatc ggcgcagcag
     2041 agcttcgcca cgtgatgaca gaccttggag agaagttaac agatgaggag gttgatgaaa
     2101 tgatcagggt agcagacatc gatggggatg gtcaggtaaa ctacgaagag tttgtacaaa
     2161 tgatgacagc gaagtgaggc gcgccgaatt cgatatcata atcaacctct ggattacaaa
     2221 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2281 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2341 ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc
     2401 tgctggacag gggctcggct gttgggcact gacaattccg tggctcgaga gatcttcgac
     2461 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     2521 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     2581 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     2641 ggaagacaat agcaggcatg agatctcacg tgcggaccga gcggccgcag gaacccctag
     2701 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     2761 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     2821 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2881 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2941 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3001 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3061 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     3121 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3181 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3241 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3301 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     3361 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     3421 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3481 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     3541 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     3601 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3661 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3721 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3781 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3841 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3901 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3961 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4021 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4081 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4141 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4201 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4261 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4321 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4381 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4441 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4501 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4561 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4621 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4681 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4741 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4801 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4861 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4921 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4981 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5041 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5101 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5161 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5221 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5281 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5341 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5401 cttttgctgg ccttttgctc acatgt
//
