LOCUS       sequence-453500-       15538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; 
            highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were 
            driven by separate 2x35s promoter; Kanamycin for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15538)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 15538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT51
FEATURES             Location/Qualifiers
     source          1..15538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             425..1219
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1795..1814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2048..2065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2080..2220
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2146..2165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2306..2328
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2824..3897)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4326..4955)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6256..6280
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6483..6499
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6926..7270
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7330..7354
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7379..7399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11105..11152
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      11183..11435
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    11480..11500
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        11939..12283
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     ncRNA           12342..12378
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(12388..12702)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLIYT
                     "
     primer_bind     12567..12583
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12593..12612
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12593..12611
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12659..12679)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12659..12677)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12698..12714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12722..12738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12746..12752,12753..12770,12771..12776))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12791..12812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     ncRNA           12854..12921
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    complement(12941..12965)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    13299..13355
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(13364..13380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13388..13404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13412..13418,13419..13436,13437..13442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13457..13478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13669..14345
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             14408..15205
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14465..14484)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     15075..15094
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    15262..15436
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    15514..15538
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccaa gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     3901 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     3961 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     4021 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     4081 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     4141 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     4201 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     4261 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     4321 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     4381 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     4441 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     4501 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     4561 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     4621 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     4681 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     4741 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     4801 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     4861 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     4921 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     4981 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     5041 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     5101 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     5161 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     5221 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     5281 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     5341 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     5401 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     5461 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     5521 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     5581 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     5641 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     5701 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     5761 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     5821 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     5881 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     5941 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     6001 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     6061 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     6121 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     6181 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     6241 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     6301 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     6361 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6421 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6481 ttgtaaaacg acggccagtg ccaagcttgg cgtgcctgca ggtcaacatg gtggagcacg
     6541 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
     6601 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
     6661 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
     6721 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
     6781 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     6841 attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
     6901 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
     6961 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
     7021 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
     7081 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     7141 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     7201 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     7261 tggagaggac ctcgactcta gaggatcccc gggtaccggg ccccccctcg aggcgcgcca
     7321 agctatcaaa caagtttgta caaaaaagca ggctccgaat tcgcccttca ccatggctcc
     7381 taagaagaag cggaaggttg gtattcacgg ggtgcctgcg gcttcaaagc tcgagaaatt
     7441 caccaactgt tattcgttga gcaaaacact gcggtttaaa gcgattccag tcggcaagac
     7501 tcaagagaat atagacaata agcggctgtt ggtggaagat gaaaagcgcg cggaagacta
     7561 caaaggggtg aagaagttgt tggacagata ctacctctct tttatcaatg atgtcttgca
     7621 ctcaatcaaa ttgaagaatc tgaacaacta catctccctc ttcagaaaga aaacaaggac
     7681 agaaaaggag aataaggaac ttgaaaattt ggagatcaat ctgaggaaag agatcgcgaa
     7741 agcctttaaa ggcaacgaag gatacaaaag tctgttcaag aaggatataa ttgagacaat
     7801 tttgccagag ttcctcgatg acaaggacga gattgcgctg gtcaattcgt tcaaccgctt
     7861 cacaacagca ttcacaggct tctttcgcaa tcgggaaaat atgttctctg aggaggcaaa
     7921 gtccacttct attgcgttca ggtgtatcaa tgagaatctc actgtgtaca tttccaacat
     7981 ggatatcttt gagaaggttg acgcaatttt tgacaagcac gaagttcagg agattaagga
     8041 gaagatcctc aattccgatt atgacgttga ggacttcttc gaaggtgagt tttttaattt
     8101 cgtgctcact caagagggta tcgacgtgta taatgcgatc atcggtgggt tcgtgactga
     8161 gtccggtgaa aagattaagg gattgaacga gtatatcaac ctttacaacc aaaagacgaa
     8221 acagaagctg ccaaagttca agcctcttta caaacaggtt ctttcagacc gcgagtcact
     8281 ctcgttctat ggggagggct acacttcgga tgaggaagtc ctggaggtgt tcaggaatac
     8341 tctcaataag aattcggaga ttttctcttc tataaaaaaa ctggaaaagt tgtttaagaa
     8401 ttttgacgaa tactctagcg ccggcatatt tgtgaaaaac ggcccggcca tatcaacgat
     8461 aagtaaagat atcttcggcg aatggaacgt gatcagagac aaatggaacg cggagtatga
     8521 cgatattcac ctgaagaaga aggctgtcgt aacggagaag tacgaggatg atcgcaggaa
     8581 aagcttcaaa aagatcggaa gtttcagcct ggaacagttg caggagtatg ctgacgccga
     8641 tcttagcgtc gtcgagaagt tgaaggagat aatcatccaa aaggtcgacg agatatataa
     8701 agtctatgga tcaagtgaaa aactgttcga cgccgacttc gttttggaga agtccctgaa
     8761 gaagaacgac gctgttgttg ccattatgaa ggatctgctc gacagcgtga agagtttcga
     8821 gaactatatt aaggcttttt tcggggaggg gaaggagact aacagagatg agtccttcta
     8881 cggagacttc gtcctcgcgt acgatatact ccttaaggta gaccacatct acgacgcaat
     8941 cagaaattac gtgacacaaa agccgtacag caaggacaag ttcaaactct acttccagaa
     9001 cccccagttc atgggcggct gggacaagga caaggaaacg gattacaggg ctacgatcct
     9061 gaggtatggt tcaaaatact acttggcgat tatggacaag aagtacgcca agtgtctcca
     9121 gaagattgac aaagacgatg tcaatggcaa ttatgagaag atcaactaca agctgcttcc
     9181 gggtccgaac aagatgctcc caaaggtttt cttcagcaag aaatggatgg cctactataa
     9241 cccaagcgag gacatccaga agatttataa gaacggtacg ttcaagaagg gcgacatgtt
     9301 caatcttaac gactgtcaca agctgatcga cttcttcaaa gactcaatta gccggtaccc
     9361 aaagtggtct aacgcctatg acttcaactt ttcggaaacc gagaagtaca aggatatagc
     9421 cggattttat agagaggtgg aagagcaggg ctacaaggtg tcattcgagt ccgccagcaa
     9481 gaaggaagtg gacaagctcg tggaagaggg taagctctac atgttccaga tttataataa
     9541 agactttagc gataagagcc acgggacacc taatctccac acaatgtatt tcaagctgct
     9601 cttcgacgag aataaccacg gccaaatcag gttgtcagga ggggctgaac tcttcatgcg
     9661 gcgcgctagc cttaagaagg aggagcttgt agtccaccct gcgaatagtc caattgcgaa
     9721 taagaacccg gacaatccta aaaagactac aacattgagc tacgacgtgt acaaggataa
     9781 gaggttttcc gaggatcagt acgagctcca catcccgatt gcgatcaaca agtgcccaaa
     9841 gaatattttc aagataaaca cagaggtgcg tgtactcctg aagcatgacg acaatcctta
     9901 cgtcattggg attgatcggg gcgagaggaa cctcctctat attgtggtgg tggacgggaa
     9961 ggggaacata gtcgaacagt actcccttaa cgaaataatt aacaatttca acggcatccg
    10021 tatcaagacc gactaccatt cgttgctgga caagaaggag aaggagagat ttgaggcgcg
    10081 gcaaaattgg acaagtatcg agaacatcaa ggaactcaaa gcaggttata tctctcaagt
    10141 tgtgcataag atatgcgagc tggttgagaa gtatgacgca gtgatcgctc ttgaggacct
    10201 caactcgggc tttaagaatt ctagagttaa agtggagaag caggtctatc aaaagttcga
    10261 gaagatgctt atagataagc tcaactacat ggtcgataag aaatcgaacc catgtgccac
    10321 cggcggcgca ctcaaaggtt accaaataac aaacaaattc gagtccttca aatcgatgag
    10381 tactcagaat gggttcatat tttatatacc ggcgtggctt acgtctaaga tcgacccgtc
    10441 aactggtttt gtcaacctgt tgaagacgaa atacacgtcc attgccgatt cgaaaaagtt
    10501 catatctagt tttgatcgta ttatgtacgt cccagaggaa gatcttttcg agtttgctct
    10561 cgactacaaa aacttttcgc ggaccgatgc ggattacatt aaaaaatgga aactctattc
    10621 gtacggcaac agaatcagga tttttcgcaa ccctaagaag aataacgtct ttgattggga
    10681 ggaagtttgc ttgactagcg cgtacaagga gctctttaat aagtatggca ttaactacca
    10741 acagggtgat atcagagcac tgctttgcga acaatctgac aaggctttct actcatcctt
    10801 catggctttg atgagcctga tgctccagat gagaaattca attacaggca gaaccgacgt
    10861 ggatttcttg atctccccgg ttaaaaattc tgatggcatc ttttacgata gcaggaacta
    10921 tgaagcgcaa gagaatgcga ttctgccaaa aaatgcagac gccaacggtg cctataacat
    10981 cgccaggaaa gtcctgtggg cgatcggcca gttcaaaaag gccgaagacg aaaaattgga
    11041 caaggtcaaa atcgctatca gcaacaaaga gtggctggag tatgctcaga catccgtaaa
    11101 gcataagcgt cctgctgcca ccaaaaaggc cggacaggct aagaaaaaga agtgagacga
    11161 ctagtggcgg ccgccgacgt ccgatcgttc aaacatttgg caataaagtt tcttaagatt
    11221 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
    11281 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
    11341 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgctcaa actaggataa
    11401 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttgatccccc ctcgacagct
    11461 tccggaaagg gcgaattcgc aactttgtat acaaaagttg ccccatggcg ttccctctag
    11521 tggcgtgcct gcaggtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
    11581 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
    11641 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
    11701 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
    11761 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
    11821 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgataacatg gtggagcacg
    11881 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
    11941 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
    12001 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
    12061 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
    12121 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
    12181 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
    12241 acccttcctc tatataagga agttcatttc atttggagag gacctcgact ctagaggatc
    12301 cccgggtacg tttggtgtta cttctgcagg gatccaaatt actgatgagt ccgtgaggac
    12361 gaaacgagta agctcgtcta atttctacta agtgtagatg agaccggcgc cgctacaggg
    12421 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    12481 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    12541 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
    12601 ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc ttttgttccc
    12661 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    12721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    12781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    12841 accggtggtc tctggccggc atggtcccag cctcctcgct ggcgccggct gggcaacatg
    12901 cttcggcatg gcgaatggga cggtacccgg ccggaattcg acccagcttt cttgtacaaa
    12961 gtggttcgat aattcttaat taactagttc tagagcggcc gccaccgcgg tggagctcga
    13021 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
    13081 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
    13141 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
    13201 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
    13261 tgtcatctat gttactgaat tcgtaatcat ggtcatagaa gcttgcatgc ctgcaggtcg
    13321 actctagagg atccccgggt accgagctcg aattcgtaat catgtcatag ctgtttcctg
    13381 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    13441 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    13501 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    13561 gaggcggttt gcgtattggc tagagcagct tgccaacatg gtggagcacg acactctcgt
    13621 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
    13681 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
    13741 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
    13801 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
    13861 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
    13921 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
    13981 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
    14041 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
    14101 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
    14161 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
    14221 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
    14281 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
    14341 gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc agatctgtcg
    14401 atcgaccatg gggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
    14461 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
    14521 tcggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
    14581 gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
    14641 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
    14701 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
    14761 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
    14821 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
    14881 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
    14941 catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat
    15001 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
    15061 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
    15121 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
    15181 tcgccttctt gacgagttct tctgagcggg actctggggt tcggatcgat cctctagcta
    15241 gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg
    15301 aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
    15361 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
    15421 tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta aaaacgtccg
    15481 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgcca
//
