LOCUS       sequence-453502-       22568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for dSpCas9 mediated gene activation for dicot plants; 
            NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven
            by AtU3 promoter; Kanamycin for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22568)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 22568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT57
FEATURES             Location/Qualifiers
     source          1..22568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             425..1219
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1795..1814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2048..2065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2080..2220
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2146..2165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2306..2328
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2824..3897)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4326..4955)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6256..6280
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6483..6499
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6926..7270
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7330..7354
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7376..7441
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7448..7468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7493..7516,7517..7519,7520..10006,10007..10009,
                     10010..11593)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             11506..11514
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             11594..11641
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             11819..11872
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11876..12262
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             12263..12283
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12326..12352
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12332..12358
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             12371..12391
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12398..12418
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12482..12538
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12554..12610
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12626..12682
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12698..12754
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12770..12826
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12842..12898
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12914..12970
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12986..13042
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13058..13114
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13130..13186
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13268..13288
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        13928..15925
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             16673..16699
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             16790..17500
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             17885..18046
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             18074..18124
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     protein_bind    18795..18815
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(19176..19481)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGL"
     primer_bind     19346..19362
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     19372..19391
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19372..19390
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(19438..19458)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(19438..19456)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(19477..19493)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    19501..19517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(19525..19531,19532..19549,19550..19555))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19570..19591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        19650..19668
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        19720..19738
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     protein_bind    complement(19971..19995)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    20329..20385
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(20394..20410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    20418..20434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(20442..20448,20449..20466,20467..20472))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    20487..20508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        20699..21375
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             21438..22235
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(21495..21514)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     22105..22124
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    22292..22466
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    22544..22568
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccaa gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     3901 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     3961 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     4021 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     4081 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     4141 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     4201 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     4261 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     4321 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     4381 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     4441 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     4501 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     4561 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     4621 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     4681 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     4741 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     4801 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     4861 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     4921 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     4981 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     5041 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     5101 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     5161 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     5221 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     5281 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     5341 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     5401 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     5461 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     5521 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     5581 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     5641 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     5701 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     5761 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     5821 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     5881 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     5941 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     6001 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     6061 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     6121 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     6181 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     6241 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     6301 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     6361 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6421 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6481 ttgtaaaacg acggccagtg ccaagcttgg cgtgcctgca ggtcaacatg gtggagcacg
     6541 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
     6601 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
     6661 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
     6721 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
     6781 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     6841 attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
     6901 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
     6961 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
     7021 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
     7081 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     7141 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     7201 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     7261 tggagaggac ctcgactcta gaggatcccc gggtaccggg ccccccctcg aggcgcgcca
     7321 agctatcaaa caagtttgta caaaaaagca ggctccgaat tcgcccttca ccatggatta
     7381 caaggaccac gacggggatt acaaggacca cgacattgat tacaaggatg atgatgacaa
     7441 gatggctccg aagaagaaga ggaaggttgg catccacggg gtgccagctg ctgacaagaa
     7501 gtactcgatc ggcctcgcta ttgggactaa ctctgttggc tgggccgtga tcaccgacga
     7561 gtacaaggtg ccctcaaaga agttcaaggt cctgggcaac accgatcggc attccatcaa
     7621 gaagaatctc attggcgctc tcctgttcga cagcggcgag acggctgagg ctacgcggct
     7681 caagcgcacc gcccgcaggc ggtacacgcg caggaagaat cgcatctgct acctgcagga
     7741 gattttctcc aacgagatgg cgaaggttga cgattctttc ttccacaggc tggaggagtc
     7801 attcctcgtg gaggaggata agaagcacga gcggcatcca atcttcggca acattgtcga
     7861 cgaggttgcc taccacgaga agtaccctac gatctaccat ctgcggaaga agctcgtgga
     7921 ctccacagat aaggcggacc tccgcctgat ctacctcgct ctggcccaca tgattaagtt
     7981 caggggccat ttcctgatcg agggggatct caacccggac aatagcgatg ttgacaagct
     8041 gttcatccag ctcgtgcaga cgtacaacca gctcttcgag gagaacccca ttaatgcgtc
     8101 aggcgtcgac gcgaaggcta tcctgtccgc taggctctcg aagtctcggc gcctcgagaa
     8161 cctgatcgcc cagctgccgg gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct
     8221 cagcctgggg ctcacgccca acttcaagtc gaatttcgat ctcgctgagg acgccaagct
     8281 gcagctctcc aaggacacat acgacgatga cctggataac ctcctggccc agatcggcga
     8341 tcagtacgcg gacctgttcc tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga
     8401 tattctcagg gtgaacaccg agattacgaa ggctccgctc tcagcctcca tgatcaagcg
     8461 ctacgacgag caccatcagg atctgaccct cctgaaggcg ctggtcaggc agcagctccc
     8521 cgagaagtac aaggagatct tcttcgatca gtcgaagaac ggctacgctg ggtacattga
     8581 cggcggggcc tctcaggagg agttctacaa gttcatcaag ccgattctgg agaagatgga
     8641 cggcacggag gagctgctgg tgaagctcaa tcgcgaggac ctcctgagga agcagcggac
     8701 attcgataac ggcagcatcc cacaccagat tcatctcggg gagctgcacg ctatcctgag
     8761 gaggcaggag gacttctacc ctttcctcaa ggataaccgc gagaagatcg agaagattct
     8821 gactttcagg atcccgtact acgtcggccc actcgctagg ggcaactccc gcttcgcttg
     8881 gatgacccgc aagtcagagg agacgatcac gccgtggaac ttcgaggagg tggtcgacaa
     8941 gggcgctagc gctcagtcgt tcatcgagag gatgacgaat ttcgacaaga acctgccaaa
     9001 tgagaaggtg ctccctaagc actcgctcct gtacgagtac ttcacagtct acaacgagct
     9061 gactaaggtg aagtatgtga ccgagggcat gaggaagccg gctttcctgt ctggggagca
     9121 gaagaaggcc atcgtggacc tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct
     9181 caaggaggac tacttcaaga agattgagtg cttcgattcg gtcgagatct ctggcgttga
     9241 ggaccgcttc aacgcctccc tggggaccta ccacgatctc ctgaagatca ttaaggataa
     9301 ggacttcctg gacaacgagg agaatgagga tatcctcgag gacattgtgc tgacactcac
     9361 tctgttcgag gaccgggaga tgatcgagga gcgcctgaag acttacgccc atctcttcga
     9421 tgacaaggtc atgaagcagc tcaagaggag gaggtacacc ggctggggga ggctgagcag
     9481 gaagctcatc aacggcattc gggacaagca gtccgggaag acgatcctcg acttcctgaa
     9541 gagcgatggc ttcgcgaacc gcaatttcat gcagctgatt cacgatgaca gcctcacatt
     9601 caaggaggat atccagaagg ctcaggtgag cggccagggg gactcgctgc acgagcatat
     9661 cgcgaacctc gctggctcgc cagctatcaa gaaggggatt ctgcagaccg tgaaggttgt
     9721 ggacgagctg gtgaaggtca tgggcaggca caagcctgag aacatcgtca ttgagatggc
     9781 ccgggagaat cagaccacgc agaagggcca gaagaactca cgcgagagga tgaagaggat
     9841 cgaggagggc attaaggagc tggggtccca gatcctcaag gagcacccgg tggagaacac
     9901 gcagctgcag aatgagaagc tctacctgta ctacctccag aatggccgcg atatgtatgt
     9961 ggaccaggag ctggatatta acaggctcag cgattacgac gtcgatgcta tcgttccaca
    10021 gtcattcctg aaggatgact ccattgacaa caaggtcctc accaggtcgg acaagaaccg
    10081 gggcaagtct gataatgttc cttcagagga ggtcgttaag aagatgaaga actactggcg
    10141 ccagctcctg aatgccaagc tgatcacgca gcggaagttc gataacctca caaaggctga
    10201 gaggggcggg ctctctgagc tggacaaggc gggcttcatc aagaggcagc tggtcgagac
    10261 acggcagatc actaagcacg ttgcgcagat tctcgactca cggatgaaca ctaagtacga
    10321 tgagaatgac aagctgatcc gcgaggtgaa ggtcatcacc ctgaagtcaa agctcgtctc
    10381 cgacttcagg aaggatttcc agttctacaa ggttcgggag atcaacaatt accaccatgc
    10441 ccatgacgcg tacctgaacg cggtggtcgg cacagctctg atcaagaagt acccaaagct
    10501 cgagagcgag ttcgtgtacg gggactacaa ggtttacgat gtgaggaaga tgatcgccaa
    10561 gtcggagcag gagattggca aggctaccgc caagtacttc ttctactcta acattatgaa
    10621 tttcttcaag acagagatca ctctggccaa tggcgagatc cggaagcgcc ccctcatcga
    10681 gacgaacggc gagacggggg agatcgtgtg ggacaagggc agggatttcg cgaccgtcag
    10741 gaaggttctc tccatgccac aagtgaatat cgtcaagaag acagaggtcc agactggcgg
    10801 gttctctaag gagtcaattc tgcctaagcg gaacagcgac aagctcatcg cccgcaagaa
    10861 ggactgggat ccgaagaagt acggcgggtt cgacagcccc actgtggcct actcggtcct
    10921 ggttgtggcg aaggttgaga agggcaagtc caagaagctc aagagcgtga aggagctgct
    10981 ggggatcacg attatggagc gctccagctt cgagaagaac ccgatcgatt tcctggaggc
    11041 gaagggctac aaggaggtga agaaggacct gatcattaag ctccccaagt actcactctt
    11101 cgagctggag aacggcagga agcggatgct ggcttccgct ggcgagctgc agaaggggaa
    11161 cgagctggct ctgccgtcca agtatgtgaa cttcctctac ctggcctccc actacgagaa
    11221 gctcaagggc agccccgagg acaacgagca gaagcagctg ttcgtcgagc agcacaagca
    11281 ttacctcgac gagatcattg agcagatttc cgagttctcc aagcgcgtga tcctggccga
    11341 cgcgaatctg gataaggtcc tctccgcgta caacaagcac cgcgacaagc caatcaggga
    11401 gcaggctgag aatatcattc atctcttcac cctgacgaac ctcggcgccc ctgctgcttt
    11461 caagtacttc gacacaacta tcgatcgcaa gaggtacaca agcactaagg aggtcctgga
    11521 cgcgaccctc atccaccagt cgattaccgg cctctacgag acgcgcatcg acctgtctca
    11581 gctcgggggc gacaagcggc cagcggcgac gaagaaggcg gggcaggcga agaagaagaa
    11641 gaagggagac ggctctggat cggggtcggg ttctggctca gtcgacgatg ctcttgacga
    11701 ttttgacctc gatatgctcg acgctcttga tgattttgat ctcgacatgc tcgatgcact
    11761 tgatgacttt gaccttgaca tgctcgacgc actcgatgac ttcgacctcg acatgcttga
    11821 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatggcgtc
    11881 aaatttcacg cagtttgttt tggttgataa cggcgggact ggcgacgtta cagtagctcc
    11941 atcaaatttt gcgaacggag tcgctgagtg gattagctca aattcaaggt cccaggccta
    12001 caaggttacc tgttctgtta ggcagagttc tgcgcaaaaa agaaaatata ccatcaaggt
    12061 tgaagtccct aaagttgcaa cacaaacagt cggtggtgtt gagctccctg tggcagcctg
    12121 gagatcttac ttaaacatgg agctaacaat tccaatattc gctacaaact ctgattgtga
    12181 actgattgtt aaggcgatgc aaggcctctt gaaagatgga aaccctatac cgtccgctat
    12241 cgcagctaac agcggtatct atcctaagaa gaagaggaag gttggctctg gatcggggtc
    12301 gggttctggc tcaggatccg gtacctatcc ctatgacgtg cccgattatg ccagcctggg
    12361 cagcggctcc cccaagaaaa aacgcaaggt ggaagatcct aagaaaaagc ggaaagtgga
    12421 cggcattggt agtgggagca acggcagcag cggatccaac ggtccgactg acgccgcgga
    12481 agaagaactt ttgagcaaga attatcatct tgagaacgaa gtggctcgtc ttaagaaagg
    12541 ttctggcagt ggagaagaac tgctttcaaa gaattaccac ctggaaaatg aggtagctag
    12601 actgaaaaag gggagcggaa gtggggagga gttgctgagc aaaaattatc atttggagaa
    12661 cgaagtagca cgactaaaga aagggtccgg atcgggtgag gagttactct cgaaaaatta
    12721 tcatctcgaa aacgaagtgg ctcggctaaa aaagggcagt ggttctggag aagagctatt
    12781 atctaaaaac taccacctcg aaaatgaggt ggcacgctta aaaaagggaa gtggcagtgg
    12841 tgaagagcta ctatccaaga attatcatct tgagaacgag gtagcgcgtt tgaagaaggg
    12901 ttccggctca ggagaggaac tgctctcgaa gaactatcat cttgaaaatg aggtcgctcg
    12961 attaaaaaag ggatcgggca gtggtgagga actactttca aagaattacc acctcgaaaa
    13021 cgaagtagct cgattaaaga aaggttcagg gtcgggtgaa gaattactga gtaaaaatta
    13081 tcatctggaa aatgaggtag cgagactaaa aaaggggagt ggttctggcg aggaattgct
    13141 atcgaaaaat tatcatcttg agaacgaagt tgctaggctc aaaaagggct caggctcagg
    13201 caccgcggta aacataggtg gtggaaccgg tccgatggat ctacagcggc cgcaaggtgg
    13261 aggtggaccc aagaagaagc gcaaggtgtg aactagttaa agagctttcg ttcgtatcat
    13321 cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca caccagaatc
    13381 ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc
    13441 ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat
    13501 ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt
    13561 ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt
    13621 ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa
    13681 cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt cagacctaga
    13741 aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca tttatgaact
    13801 ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt ataattatag
    13861 ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt tatgacttgc
    13921 ccgtacgctg cagtgcagcg tgacccggtc gtgcccctct ctagagataa tgagcattgc
    13981 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
    14041 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
    14101 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
    14161 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
    14221 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
    14281 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
    14341 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
    14401 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
    14461 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
    14521 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
    14581 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
    14641 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
    14701 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
    14761 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
    14821 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gatctccccc
    14881 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc cccccccccc
    14941 tctctacctt ctctagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct
    15001 gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg ttcgtacacg
    15061 gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg
    15121 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
    15181 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
    15241 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
    15301 ggtcgttcta gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat
    15361 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
    15421 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
    15481 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
    15541 tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
    15601 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
    15661 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
    15721 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
    15781 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
    15841 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
    15901 ctgttgtttg gtgttacttc tgcagggcgc catggggccg gacatagtca tgacacaaag
    15961 tccgagttcg ctttctgcga gtgttgggga tagagttacg ataacctgca gatctagtac
    16021 gggggccgtc accacgtcga attacgcgtc atgggtccaa gaaaagccag gcaaactgtt
    16081 taagggtctt ataggtggca cgaacaaccg ggctcccgga gtcccttcca ggtttagtgg
    16141 atcacttatt ggtgacaagg caacgctgac gatatcttcc ctccagccag aagatttcgc
    16201 gacctacttt tgtgcacttt ggtattctaa ccactgggtc tttggccaag ggacgaaggt
    16261 cgagctcaaa cggggtggcg gtgggagcgg cgggggrgga tcagggggsg gaggttcgag
    16321 tggcgggggg agcgaagtca aactcctcga gtccggtgga ggattggtgc aaccaggtgg
    16381 ttcattgaag ctctcgtgtg cagtttctgg tttctctttg acagactacg gtgtcaattg
    16441 ggtccggcag gcaccggggc gcggtcttga gtggatcggc gttatctggg gcgacggaat
    16501 aacagattat aactctgctt tgaaggacag attcatcatt agcaaggaca acgggaagaa
    16561 cacggtgtac ctccagatga gcaaagtgcg cagtgatgat actgccctgt attactgcgt
    16621 cacaggtttg tttgattact ggggtcaagg cactcttgtg acagtctctt cgtatcctta
    16681 tgacgtgccc gactacgctg gcggaggggg cggcagcgga ggcggtggat ccggaggcgg
    16741 tggctcaggg ggcggcgggt cgcttgatcc tggtggcgga ggttctggga gcaaaggaga
    16801 ggagctcttt acaggtgtcg tccctatttt ggtggagctt gatggagatg tcaatggcca
    16861 taaattttcc gtcaggggcg aaggggaggg ggacgcgact aatggaaaat tgaccctcaa
    16921 gtttatctgc acgacaggaa agttgccggt cccctggccc actctggtta ccaccctcac
    16981 ttatggggtc cagtgcttta gcagataccc tgatcatatg aaacgccatg acttcttcaa
    17041 atcagccatg ccagagggtt atgtgcagga aagaactata agctttaagg acgatgggac
    17101 gtacaaaacg cgggcagagg ttaagtttga gggggatact cttgtgaata ggatcgaact
    17161 taagggcatc gattttaaag aagacggtaa tattcttggc cacaagttgg agtataattt
    17221 caattctcac aacgtgtaca taacagcaga taaacagaaa aatgggatca aggccaattt
    17281 taaaattagg cataatgttg aagatgggtc tgtgcaactg gcagatcatt atcagcagaa
    17341 tactccaatt ggcgatggcc ctgtcttgtt gcctgacaat cattaccttt ccacgcagtc
    17401 agtcctgtcc aaagatccga atgagaagcg ggaccacatg gtcttgctcg aattcgttac
    17461 tgccgccgga atcactcatg gcatggacga gctttacaag ggaggtggaa ggactggagg
    17521 tggcggaggg gggctgcttg accccggaac ccctatggat gccgacttgg tggcctcctc
    17581 cacggttgtt tgggaacagg atgccgaccc ttttgcgggc actgctgacg attttcccgc
    17641 tttcaatgag gaggagttgg cttggctgat ggaacttctc ccacagggtg gtagtggtgg
    17701 cctcctggat ccggggacac cgatggacgc agacctggtg gcgtcgtcaa cggttgtgtg
    17761 ggaacaggat gctgatccgt tcgcaggaac tgccgacgat tttcctgcat tcaacgaaga
    17821 ggaactcgca tggctcatgg agcttctgcc tcagggatcg ggtggcgggt cgcgaacgga
    17881 agaatacaag cttatattga acggtaagac actgaaaggt gaaactacga cagaagccgt
    17941 tgacgcggca actgcggaga aagtctttaa gcagtatgct aacgataatg gcgtggacgg
    18001 ggagtggacg tatgacgacg caaccaaaac attcacggtc accgaggggg gcggcagcgg
    18061 aggcggtacc tcgcccaaaa cccggagacg ccctaggcgc tcgcagcgca aaagacctcc
    18121 cacgtaaaga gctttcgttc gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat
    18181 cagtttcatt gcgcacacac cagaatccta ctgagtttga gtattatggc attgggaaaa
    18241 ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg
    18301 ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg
    18361 ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa
    18421 ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa
    18481 tgaccgaagt taatatgagg agtaaaacac ttgtagttgt accattatgc ttattcacta
    18541 ggcaacaaat atattttcag acctagaaaa gctgcaaatg ttactgaata caagtatgtc
    18601 ctcttgtgtt ttagacattt atgaactttc ctttatgtaa ttttccagaa tccttgtcag
    18661 attctaatca ttgctttata attatagtta tactcatgga tttgtagttg agtatgaaaa
    18721 tattttttaa tgcattttat gacttgccaa ttgttccgga actctagata agcttaccgg
    18781 aaagggcgaa ttcgcaactt tgtatacaaa agttgcccca tggcgttccc tctagataac
    18841 gcaggatcct atttacttta aatttttctt atggctcagc ctgtgatgga taactgaatc
    18901 aaacaaatgg cgtctgggtt taagaacatc tgttttggct atgttggacg aaacaagtga
    18961 acttttagga tcaacttccg tttatatacg gagcttatat cgagcaataa gataagtggg
    19021 ctttttatgt aatttaatgg gctatcgtcc atatattcac taatacccat gcccagtacc
    19081 catgtatgcg tttcatataa gctcctaatt tctcccacat cgctcaaatc taaacaaatc
    19141 ttgttgtata tataacactg agggagcacc attggtcaga gaccggcgcc gctacagggc
    19201 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    19261 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    19321 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
    19381 tcactatagg gcgaattggg taccgggccc cccctcgagg tcctccagct tttgttccct
    19441 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    19501 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    19561 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    19621 ccggtggtct ctgttttaga gctaggccaa catgaggatc acccatgtct gcagggccta
    19681 gcaagttaaa ataaggctag tccgttatca acttggccaa catgaggatc acccatgtct
    19741 gcagggccaa gtggcaccga gtcggtgctt ttttttccct ttcctttttt cttttttttg
    19801 ccataaactt aaatttgtat atcgatcatt gtagatattg aaaacctaga acaaaccaac
    19861 atccatgtga atgtctttca tgactgattt agagataatt cttgaatttt ggaactagaa
    19921 tctataatga gcctaaatta aaacattgtg aactagtaag ggcgaattcg acccagcttt
    19981 cttgtacaaa gtggttcgat aattcttaat taactagttc tagagcggcc gccaccgcgg
    20041 tggagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
    20101 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
    20161 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
    20221 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
    20281 tcgcgcgcgg tgtcatctat gttactgaat tcgtaatcat ggtcatagaa gcttgcatgc
    20341 ctgcaggtcg actctagagg atccccgggt accgagctcg aattcgtaat catgtcatag
    20401 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    20461 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    20521 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    20581 cgcgcgggga gaggcggttt gcgtattggc tagagcagct tgccaacatg gtggagcacg
    20641 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
    20701 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
    20761 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
    20821 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc
    20881 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
    20941 attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
    21001 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
    21061 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
    21121 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
    21181 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
    21241 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
    21301 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
    21361 gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc
    21421 agatctgtcg atcgaccatg gggattgaac aagatggatt gcacgcaggt tctccggccg
    21481 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
    21541 ccgccgtgtt tcggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
    21601 ccggtgccct gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg
    21661 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
    21721 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
    21781 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    21841 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    21901 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    21961 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc
    22021 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    22081 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    22141 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    22201 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcggatcgat
    22261 cctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
    22321 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
    22381 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
    22441 caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta
    22501 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
    22561 tcctgcca
//
