LOCUS       sequence-453529-        3057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: replication origin (pWV01) for the 
            construction of Level 1 plasmids. **Plasmid has been discontinued. 
            See Comments section below for suggestions from depositing lab..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPO4
FEATURES             Location/Qualifiers
     source          1..3057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..302)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2021..2115)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2136..2795)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2710..2729
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2957..2975
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2979..3022)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3025..3046
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ctcgagctag agcacacggt ttaacgactt aattacgaag taaataagtc tagtgtgtta
       61 gactttatga aatctatata cgtttatata tatttattat ccgatttttt attaaaacgt
      121 ctcaaaatcg tttctgagac gttttagcgt ttatttcgtt tagttatcgg cataatcgtt
      181 aaaacaggcg ttatcgtagc gtaaaagccc ttgagcgtag cgtggctttg cagcgaagat
      241 gttgtctgtt agattatgaa agccgatgac tgaatgaaat aataagcgca gcgcccttct
      301 atttcggttg gaggaggctc aagggagtat gagggaatga aattccctca tgggtttgat
      361 tttaaaaatt gcttgcaatt ttgccgagcg gtagcgctgg aaaatttttg aaaaaaattt
      421 ggaatttgga aaaaaatggg gggaaaggaa gcgaattttg cttccgtact acgacccccc
      481 cattaagtgc cgagtgccaa tttttgtgcc aaaaacgctc tatcccaact ggctcaaggg
      541 tttaaggggt ttttcaatcg ccaacgaatc gccaacgttt tcgccaacgt tttcgccaac
      601 gttttttata aatctatatt taagtagctt tattgttgtt tttatgatta caaagtgata
      661 cactaacttt ataaaattat ttgattggag ttttttaaat ggtgatttca gaatcgaaaa
      721 aaagagttat gatttctctg acaaaagagc aagataaaaa attaacagat atggcgaaac
      781 aaaaaggttt ttcaaaatct gcggttgcgg cgttagctat agaagaatat gcaagaaagg
      841 aatcagaaca aaaaaaataa gcgaaagctc gcgtttttag aaggatacga gttttcgcta
      901 cttgtttttg ataaggtaat tatatcatgg ctattaaaaa tactaaagct agaaattttg
      961 gatttttatt atatcctgac tcaattccta atgattggaa agaaaaatta gagagtttgg
     1021 gcgtatctat ggctgtcagt cctttacacg atatggacga aaaaaaagat aaagatacat
     1081 ggaatagtag tgatgttata cgaaatggaa agcactataa aaaaccacac tatcacgtta
     1141 tatatattgc acgaaatcct gtaacaatag aaagcgttag gaacaagatt aagcgaaaat
     1201 tggggaatag ttcagttgct catgttgaga tacttgatta tatcaaagtt tcatatgaat
     1261 atttgactca tgaatcaaag gacgctattg ctaagaataa acatatatac gacaaaaaag
     1321 atattttgaa cattaatgat tttgatattg accgctatat aacacttgat gaaagccaaa
     1381 aaagagaatt gaagaattta cttttagata tagtggatga ctataatttg gtaaatacaa
     1441 aagatttaat ggcttttatt cgccttaggg gagcggagtt tggaatttta aatacgaatg
     1501 atgtaaaaga tattgtttca acaaactcta gcgcctttag attatggttt gagggcaatt
     1561 atcagtgtgg atatagagca agttatgcaa aggttcttga tgctgaaacg ggggaaataa
     1621 aatgacaaac aaagaaaaag agttatttgc tgaaaatgag gaattaaaaa aagaaattaa
     1681 ggacttaaaa gagcgtattg aaagatacag agaaatggaa gttgaattaa gtacaacaat
     1741 agatttattg agaggaggga ttattgaata aataaaagcc ccctgacgaa agtcgaaggg
     1801 ggtttttatt ttggtttgat gttgcgatta atagcaatac aattgcaata aacaaaatga
     1861 tcttccttca ggttatgact gcttgagacc cacgttaagg gattttggtc atgagattat
     1921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1981 gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg
     2041 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     2101 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcgtcg
     2161 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     2221 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     2281 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     2341 ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt agggaaatag
     2401 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     2461 tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg
     2521 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     2581 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     2641 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     2701 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     2761 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     2821 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     2881 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
     2941 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     3001 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagcgat gagtggt
//
