LOCUS       sequence-453531-       19173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for SpRY-D10A based A-to-G base editing for monocot 
            plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 
            and the sgRNA was driven by OsU3; Hygromycin for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19173)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 19173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT40
FEATURES             Location/Qualifiers
     source          1..19173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    34..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(58..64,65..82,83..88))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        315..991
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     928..948
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1058..2083
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2376..2400
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4024..4818
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4905..5493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5394..5413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5647..5664
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5679..5819
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5745..5764)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5905..5927
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6163..6357
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6423..7496)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7925..8554)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9849..9873
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10076..10092
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10108..12105
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    12148..12172
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             12212..12232
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12833..12898
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             12905..12925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             16963..16971
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             17051..17098
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    17773..17793
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        17830..18207
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             complement(18178..18510)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLCHG
                     SSAQLF"
     primer_bind     18375..18391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18401..18420
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        18401..18419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(18467..18487)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(18467..18485)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(18506..18522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    18530..18546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(18554..18560,18561..18578,18579..18584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18599..18620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        18662..18737
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(18826..18850)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      18914..19166
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      541 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
      601 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
      661 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
      721 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
      781 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      841 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      901 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      961 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     1021 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     1321 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
     2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
     2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2761 tcttgttata attagggaag gtgcgaacaa gtccctgata tgagatcatg tttgtcatct
     2821 ggagccatag aacagggttc atcatgagtc atcaacttac cttcgccgac agtgaattca
     2881 gcagtaagcg ccgtcagacc agaaaagaga ttttcttgtc ccgcatggag cagattctgc
     2941 catggcaaaa catggtggaa gtcatcgagc cgttttaccc caaggctggt aatggccggc
     3001 gaccttatcc gctggaaacc atgctacgca ttcactgcat gcagcattgg tacaacctga
     3061 gcgatggcgc gatggaagat gctctgtacg aaatcgcctc catgcgtctg tttgcccggt
     3121 tatccctgga tagcgccttg ccggaccgca ccaccatcat gaatttccgc cacctgctgg
     3181 agcagcatca actggcccgc caattgttca agaccatcaa tcgctggctg gccgaagcag
     3241 gcgtcatgat gactcaaggc accttggtcg atgccaccat cattgaggca cccagctcga
     3301 ccaagaacaa agagcagcaa cgcgatccgg agatgcatca gaccaagaaa ggcaatcagt
     3361 ggcactttgg catgaaggcc cacattggtg tcgatgccaa gagtggcctg acccacagcc
     3421 tggtcaccac cgcggccaac gagcatgacc tcaatcagct gggtaatctg ctgcatggag
     3481 aggagcaatt tgtctcagcc gatgccggct accaaggggc gccacagcgc gaggagctgg
     3541 ccgaggtgga tgtggactgg ctgatcgccg agcgccccgg caaggtaaga accttgaaac
     3601 agcatccacg caagaacaaa acggccatca acatcgaata catgaaagcc agcatccggg
     3661 ccagggtgga gcacccattt cgcatcatca agcgacagtt cggcttcgtg aaagccagat
     3721 acaaggggtt gctgaaaaac gataaccaac tggcgatgtt attcacgctg gccaacctgt
     3781 ttcgggcgga ccaaatgata cgtcagtggg agagatctca ctaaaaactg gggataacgc
     3841 cttaaatggc gaagaaacgg tctaaatagg ctgattcaag gcatttacgg gagaaaaaat
     3901 cggctcaaac atgaagaaat gaaatgactg agtcagccga gaagaatttc cccgcttatt
     3961 cgcaccttcc ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
     4021 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
     4081 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
     4141 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
     4201 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
     4261 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
     4321 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
     4381 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
     4441 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
     4501 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
     4561 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
     4621 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
     4681 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
     4741 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
     4801 ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg tgagttttcg
     4861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5041 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     5701 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     5761 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     5821 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5881 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5941 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     6001 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
     6061 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     6121 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     6181 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     6241 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     6301 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     6361 aacagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     6421 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     6481 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
     6541 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
     6601 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
     6661 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
     6721 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
     6781 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
     6841 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
     6901 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
     6961 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
     7021 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
     7081 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
     7141 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
     7201 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
     7261 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
     7321 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
     7381 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
     7441 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
     7501 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc cagccgctta
     7561 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     7621 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     7681 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     7741 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     7801 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     7861 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     7921 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     7981 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     8041 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     8101 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     8161 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     8221 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     8281 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     8341 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     8401 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     8461 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     8521 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     8581 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     8641 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     8701 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     8761 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     8821 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     8881 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct aggcaccgcg ccgcctggcc
     8941 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
     9001 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
     9061 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
     9121 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
     9181 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
     9241 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
     9301 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
     9361 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
     9421 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
     9481 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
     9541 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
     9601 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
     9661 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
     9721 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
     9781 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
     9841 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg
     9901 gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac
     9961 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    10021 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    10081 acgacggcca gtgccaagct tgcatgcctg cagtgcagcg tgacccggtc gtgcccctct
    10141 ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc
    10201 acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga
    10261 ataatataat ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca
    10321 gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac agttttatct
    10381 ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca
    10441 tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt
    10501 tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt
    10561 tagttttttt atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta
    10621 aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga
    10681 taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca
    10741 gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc
    10801 ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt
    10861 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg
    10921 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
    10981 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
    11041 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
    11101 cgtcctcccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatggttag
    11161 ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt
    11221 gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt
    11281 gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga
    11341 tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa
    11401 tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg
    11461 atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattaattc tgtttcaaac
    11521 tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac
    11581 gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt
    11641 ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt
    11701 gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat
    11761 ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg
    11821 gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata
    11881 catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa
    11941 taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag
    12001 cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt
    12061 ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcaggtcga ctctagagga
    12121 tcccctcgag gcgcgccaag ctatcaaaca agtttgtaca aaaaagcagg ctccgatgaa
    12181 acggacagcc gacggaagcg agttcgagtc accaaagaag aagcggaaag tctctgaggt
    12241 ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacggga
    12301 tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg
    12361 ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag
    12421 acagggcggc ctggtcatgc agaactacag actgattgac gccaccctgt acgtgacatt
    12481 cgagccttgc gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt
    12541 tggcgtgagg aactcaaaaa gaggcgccgc aggctccctg atgaacgtgc tgaactaccc
    12601 cggcatgaat caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct
    12661 gctgtgcgat ttctatcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag
    12721 ctccatcaac tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag
    12781 cgagagcgca acacctgaaa gcagcggggg cagcagcggg gggtcaacca tggattacaa
    12841 ggaccacgac ggggattaca aggaccacga cattgattac aaggatgatg atgacaagat
    12901 ggctccgaag aagaagagga aggttggcat ccacggggtg ccagctgctg acaagaagta
    12961 ctcgatcggc ctcgctattg ggactaactc tgttggctgg gccgtgatca ccgacgagta
    13021 caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa
    13081 gaatctcatt ggcgctctcc tgttcgacag cggcgagacg gctgagagga cgcggctcaa
    13141 gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc atctgctacc tgcaggagat
    13201 tttctccaac gagatggcga aggttgacga ttctttcttc cacaggctgg aggagtcatt
    13261 cctcgtggag gaggataaga agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga
    13321 ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc tcgtggactc
    13381 cacagataag gcggacctcc gcctgatcta cctcgctctg gcccacatga ttaagttcag
    13441 gggccatttc ctgatcgagg gggatctcaa cccggacaat agcgatgttg acaagctgtt
    13501 catccagctc gtgcagacgt acaaccagct cttcgaggag aaccccatta atgcgtcagg
    13561 cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag tctcggcgcc tcgagaacct
    13621 gatcgcccag ctgccgggcg agaagaagaa cggcctgttc gggaatctca ttgcgctcag
    13681 cctggggctc acgcccaact tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca
    13741 gctctccaag gacacatacg acgatgacct ggataacctc ctggcccaga tcggcgatca
    13801 gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac gccatcctcc tgtctgatat
    13861 tctcagggtg aacaccgaga ttacgaaggc tccgctctca gcctccatga tcaagcgcta
    13921 cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtcaggcagc agctccccga
    13981 gaagtacaag gagatcttct tcgatcagtc gaagaacggc tacgctgggt acattgacgg
    14041 cggggcctct caggaggagt tctacaagtt catcaagccg attctggaga agatggacgg
    14101 cacggaggag ctgctggtga agctcaatcg cgaggacctc ctgaggaagc agcggacatt
    14161 cgataacggc agcatcccac accagattca tctcggggag ctgcacgcta tcctgaggag
    14221 gcaggaggac ttctaccctt tcctcaagga taaccgcgag aagatcgaga agattctgac
    14281 tttcaggatc ccgtactacg tcggcccact cgctaggggc aactcccgct tcgcttggat
    14341 gacccgcaag tcagaggaga cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg
    14401 cgctagcgct cagtcgttca tcgagaggat gacgaatttc gacaagaacc tgccaaatga
    14461 gaaggtgctc cctaagcact cgctcctgta cgagtacttc acagtctaca acgagctgac
    14521 taaggtgaag tatgtgaccg agggcatgag gaagccggct ttcctgtctg gggagcagaa
    14581 gaaggccatc gtggacctcc tgttcaagac caaccggaag gtcacggtta agcagctcaa
    14641 ggaggactac ttcaagaaga ttgagtgctt cgattcggtc gagatctctg gcgttgagga
    14701 ccgcttcaac gcctccctgg ggacctacca cgatctcctg aagatcatta aggataagga
    14761 cttcctggac aacgaggaga atgaggatat cctcgaggac attgtgctga cactcactct
    14821 gttcgaggac cgggagatga tcgaggagcg cctgaagact tacgcccatc tcttcgatga
    14881 caaggtcatg aagcagctca agaggaggag gtacaccggc tgggggaggc tgagcaggaa
    14941 gctcatcaac ggcattcggg acaagcagtc cgggaagacg atcctcgact tcctgaagag
    15001 cgatggcttc gcgaaccgca atttcatgca gctgattcac gatgacagcc tcacattcaa
    15061 ggaggatatc cagaaggctc aggtgagcgg ccagggggac tcgctgcacg agcatatcgc
    15121 gaacctcgct ggctcgccag ctatcaagaa ggggattctg cagaccgtga aggttgtgga
    15181 cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac atcgtcattg agatggcccg
    15241 ggagaatcag accacgcaga agggccagaa gaactcacgc gagaggatga agaggatcga
    15301 ggagggcatt aaggagctgg ggtcccagat cctcaaggag cacccggtgg agaacacgca
    15361 gctgcagaat gagaagctct acctgtacta cctccagaat ggccgcgata tgtatgtgga
    15421 ccaggagctg gatattaaca ggctcagcga ttacgacgtc gatcatatcg ttccacagtc
    15481 attcctgaag gatgactcca ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg
    15541 caagtctgat aatgttcctt cagaggaggt cgttaagaag atgaagaact actggcgcca
    15601 gctcctgaat gccaagctga tcacgcagcg gaagttcgat aacctcacaa aggctgagag
    15661 gggcgggctc tctgagctgg acaaggcggg cttcatcaag aggcagctgg tcgagacacg
    15721 gcagatcact aagcacgttg cgcagattct cgactcacgg atgaacacta agtacgatga
    15781 gaatgacaag ctgatccgcg aggtgaaggt catcaccctg aagtcaaagc tcgtctccga
    15841 cttcaggaag gatttccagt tctacaaggt tcgggagatc aacaattacc accatgccca
    15901 tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc aagaagtacc caaagctcga
    15961 gagcgagttc gtgtacgggg actacaaggt ttacgatgtg aggaagatga tcgccaagtc
    16021 ggagcaggag attggcaagg ctaccgccaa gtacttcttc tactctaaca ttatgaattt
    16081 cttcaagaca gagatcactc tggccaatgg cgagatccgg aagcgccccc tcatcgagac
    16141 gaacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa
    16201 ggttctctcc atgccacaag tgaatatcgt caagaagaca gaggtccaga ctggcgggtt
    16261 ctctaaggag tcaattaggc ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga
    16321 ctgggatccg aagaagtacg gcgggttcct gtggcccact gtggcctact cggtcctggt
    16381 tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg agctgctggg
    16441 gatcacgatt atggagcgct ccagcttcga gaagaacccg atcgatttcc tggaggcgaa
    16501 gggctacaag gaggtgaaga aggacctgat cattaagctc cccaagtact cactcttcga
    16561 gctggagaac ggcaggaagc ggatgctggc ttccgctaag cagctgcaga aggggaacga
    16621 gctggctctg ccgtccaagt atgtgaactt cctctacctg gcctcccact acgagaagct
    16681 caagggcagc cccgaggaca acgagcagaa gcagctgttc gtcgagcagc acaagcatta
    16741 cctcgacgag atcattgagc agatttccga gttctccaag cgcgtgatcc tggccgacgc
    16801 gaatctggat aaggtcctct ccgcgtacaa caagcaccgc gacaagccaa tcagggagca
    16861 ggctgagaat atcattcatc tcttcaccct gacgaggctc ggcgccccta gggctttcaa
    16921 gtacttcgac acaactatcg atccgaagca gtacaggagc actaaggagg tcctggacgc
    16981 gaccctcatc caccagtcga ttaccggcct ctacgagacg cgcatcgacc tgtctcagct
    17041 cgggggcgac aagcggccag cggcgacgaa gaaggcgggg caggcgaaga agaagaagtg
    17101 agctcagagc tttcgttcgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca
    17161 gtttcattgc gcacacacca gaatcctact gagtttgagt attatggcat tgggaaaact
    17221 gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct
    17281 atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt
    17341 cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt
    17401 ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg
    17461 accgaagtta atatgaggag taaaacactt gtagttgtac cattatgctt attcactagg
    17521 caacaaatat attttcagac ctagaaaagc tgcaaatgtt actgaataca agtatgtcct
    17581 cttgtgtttt agacatttat gaactttcct ttatgtaatt ttccagaatc cttgtcagat
    17641 tctaatcatt gctttataat tatagttata ctcatggatt tgtagttgag tatgaaaata
    17701 ttttttaatg cattttatga cttgccaatt gttccggaac tctagataag cttaccggaa
    17761 agggcgaatt cgcaactttg tatacaaaag ttgccccatg gcgttccctc tagataacgc
    17821 aggatcctaa agggatcttt aaacatacga acagatcact taaagttctt ctgaagcaac
    17881 ttaaagttat caggcatgca tggatcttgg aggaatcaga tgtgcagtca gggaccatag
    17941 cacaggacag gcgtcttcta ctggtgctac cagcaaatgc tggaagccgg gaacactggg
    18001 tacgttggaa accacgtgat gtggagtaag ataaactgta ggagaaaagc atttcgtagt
    18061 gggccatgaa gcctttcagg acatgtattg cagtatgggc cggcccatta cgcaattgga
    18121 cgacaacaaa gactagtatt agtaccacct cggctatcca catagatcaa agctggttta
    18181 aaagagttgt gcagatgatc cgtggcagag accggcgccg ctacagggcg cgtcccattc
    18241 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    18301 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    18361 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
    18421 cgaattgggt accgggcccc ccctcgaggt cctccagctt ttgttccctt tagtgagggt
    18481 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    18541 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    18601 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccac cggtggtctc
    18661 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
    18721 tggcaccgag tcggtgcttt ttttgtccct tcgaagggca attctgcaga tatccatcac
    18781 actggcggcc gctcgaggtc gagggtatcg ataagcttga attcgaccca gctttcttgt
    18841 acaaagtggt tcgataattc ttaattaact agttctagag cggccgccac cgcggtggag
    18901 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
    18961 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
    19021 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
    19081 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
    19141 cgcggtgtca tctatgttac tagatcggga att
//
