LOCUS       sequence-453575-       17272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for temperature tolerance LbCas12a-D156R based 
            mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the 
            crRNA were driven by separate ZmUbi1 promoter; BASTA for plants 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 17272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT43
FEATURES             Location/Qualifiers
     source          1..17272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    283..307
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     510..526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        542..2539
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2582..2606
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2631..2651
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6357..6404
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6435..6687
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6732..6752
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     ncRNA           8748..8784
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(8794..9108)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLIYT
                     "
     primer_bind     8973..8989
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8999..9018
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8999..9017
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9065..9085)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9065..9083)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9104..9120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9128..9144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9152..9158,9159..9176,9177..9182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9197..9218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     ncRNA           9260..9327
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    complement(9347..9371)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9439..9455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9463..9479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9487..9493,9494..9511,9512..9517))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9532..9553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9744..10420
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10357..10377
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10465..11016
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11023..11197
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11275..11299
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11724..12518
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12605..13193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13094..13113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13347..13364
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13379..13519
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13445..13464)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13605..13627
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13863..14057
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14123..15196)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15625..16254)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
       61 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
      121 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
      181 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
      241 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
      301 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
      361 gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      421 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      481 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
      541 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      601 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      661 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      721 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      781 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      841 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      901 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      961 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     1021 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     1081 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     1141 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     1201 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     1261 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     1321 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     1381 cgctccttcg ctttcccttc cycgcccgcc gtaataaata gacaccccct ccacaccctc
     1441 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     1501 mcccgtcggc acctccgctt caaggtacgc cgctcgtccc cccccccccc cccctctcta
     1561 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     1621 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     1681 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     1741 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     1801 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     1861 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     1921 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     1981 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     2041 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     2101 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     2161 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     2221 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     2281 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     2341 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     2401 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     2461 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     2521 tttggtgtta cttctgcagg tcgactctag aggatcccct cgaggcgcgc caagctatca
     2581 aacaagtttg tacaaaaaag caggctccga attcgccctt caccatggct cctaagaaga
     2641 agcggaaggt tggtattcac ggggtgcctg cggcttcaaa gctcgagaaa ttcaccaact
     2701 gttattcgtt gagcaaaaca ctgcggttta aagcgattcc agtcggcaag actcaagaga
     2761 atatagacaa taagcggctg ttggtggaag atgaaaagcg cgcggaagac tacaaagggg
     2821 tgaagaagtt gttggacaga tactacctct cttttatcaa tgatgtcttg cactcaatca
     2881 aattgaagaa tctgaacaac tacatctccc tcttcagaaa gaaaacaagg acagaaaagg
     2941 agaataagga acttgaaaat ttggagatca atctgaggaa agagatcgcg aaagccttta
     3001 aaggcaacga aggatacaaa agtctgttca agaaggatat aattgagaca attttgccag
     3061 agttcctcga tgacaaggac gagattgcgc tggtcaattc gttcaacgga ttcacaacag
     3121 cattcacagg cttctttcgc aatcgggaaa atatgttctc tgaggaggca aagtccactt
     3181 ctattgcgtt caggtgtatc aatgagaatc tcactaggta catttccaac atggatatct
     3241 ttgagaaggt tgacgcaatt tttgacaagc acgaagttca ggagattaag gagaagatcc
     3301 tcaattccga ttatgacgtt gaggacttct tcgaaggtga gttttttaat ttcgtgctca
     3361 ctcaagaggg tatcgacgtg tataatgcga tcatcggtgg gttcgtgact gagtccggtg
     3421 aaaagattaa gggattgaac gagtatatca acctttacaa ccaaaagacg aaacagaagc
     3481 tgccaaagtt caagcctctt tacaaacagg ttctttcaga ccgcgagtca ctctcgttct
     3541 atggggaggg ctacacttcg gatgaggaag tcctggaggt gttcaggaat actctcaata
     3601 agaattcgga gattttctct tctataaaaa aactggaaaa gttgtttaag aattttgacg
     3661 aatactctag cgccggcata tttgtgaaaa acggcccggc catatcaacg ataagtaaag
     3721 atatcttcgg cgaatggaac gtgatcagag acaaatggaa cgcggagtat gacgatattc
     3781 acctgaagaa gaaggctgtc gtaacggaga agtacgagga tgatcgcagg aaaagcttca
     3841 aaaagatcgg aagtttcagc ctggaacagt tgcaggagta tgctgacgcc gatcttagcg
     3901 tcgtcgagaa gttgaaggag ataatcatcc aaaaggtcga cgagatatat aaagtctatg
     3961 gatcaagtga aaaactgttc gacgccgact tcgttttgga gaagtccctg aagaagaacg
     4021 acgctgttgt tgccattatg aaggatctgc tcgacagcgt gaagagtttc gagaactata
     4081 ttaaggcttt tttcggggag gggaaggaga ctaacagaga tgagtccttc tacggagact
     4141 tcgtcctcgc gtacgatata ctccttaagg tagaccacat ctacgacgca atcagaaatt
     4201 acgtgacaca aaagccgtac agcaaggaca agttcaaact ctacttccag aacccccagt
     4261 tcatgggcgg ctgggacaag gacaaggaaa cggattacag ggctacgatc ctgaggtatg
     4321 gttcaaaata ctacttggcg attatggaca agaagtacgc caagtgtctc cagaagattg
     4381 acaaagacga tgtcaatggc aattatgaga agatcaacta caagctgctt ccgggtccga
     4441 acaagatgct cccaaaggtt ttcttcagca agaaatggat ggcctactat aacccaagcg
     4501 aggacatcca gaagatttat aagaacggta cgttcaagaa gggcgacatg ttcaatctta
     4561 acgactgtca caagctgatc gacttcttca aagactcaat tagccggtac ccaaagtggt
     4621 ctaacgccta tgacttcaac ttttcggaaa ccgagaagta caaggatata gccggatttt
     4681 atagagaggt ggaagagcag ggctacaagg tgtcattcga gtccgccagc aagaaggaag
     4741 tggacaagct cgtggaagag ggtaagctct acatgttcca gatttataat aaagacttta
     4801 gcgataagag ccacgggaca cctaatctcc acacaatgta tttcaagctg ctcttcgacg
     4861 agaataacca cggccaaatc aggttgtcag gaggggctga actcttcatg cggcgcgcta
     4921 gccttaagaa ggaggagctt gtagtccacc ctgcgaatag tccaattgcg aataagaacc
     4981 cggacaatcc taaaaagact acaacattga gctacgacgt gtacaaggat aagaggtttt
     5041 ccgaggatca gtacgagctc cacatcccga ttgcgatcaa caagtgccca aagaatattt
     5101 tcaagataaa cacagaggtg cgtgtactcc tgaagcatga cgacaatcct tacgtcattg
     5161 ggattgatcg gggcgagagg aacctcctct atattgtggt ggtggacggg aaggggaaca
     5221 tagtcgaaca gtactccctt aacgaaataa ttaacaattt caacggcatc cgtatcaaga
     5281 ccgactacca ttcgttgctg gacaagaagg agaaggagag atttgaggcg cggcaaaatt
     5341 ggacaagtat cgagaacatc aaggaactca aagcaggtta tatctctcaa gttgtgcata
     5401 agatatgcga gctggttgag aagtatgacg cagtgatcgc tcttgaggac ctcaactcgg
     5461 gctttaagaa ttctagagtt aaagtggaga agcaggtcta tcaaaagttc gagaagatgc
     5521 ttatagataa gctcaactac atggtcgata agaaatcgaa cccatgtgcc accggcggcg
     5581 cactcaaagg ttaccaaata acaaacaaat tcgagtcctt caaatcgatg agtactcaga
     5641 atgggttcat attttatata ccggcgtggc ttacgtctaa gatcgacccg tcaactggtt
     5701 ttgtcaacct gttgaagacg aaatacacgt ccattgccga ttcgaaaaag ttcatatcta
     5761 gttttgatcg tattatgtac gtcccagagg aagatctttt cgagtttgct ctcgactaca
     5821 aaaacttttc gcggaccgat gcggattaca ttaaaaaatg gaaactctat tcgtacggca
     5881 acagaatcag gatttttcgc aaccctaaga agaataacgt ctttgattgg gaggaagttt
     5941 gcttgactag cgcgtacaag gagctcttta ataagtatgg cattaactac caacagggtg
     6001 atatcagagc actgctttgc gaacaatctg acaaggcttt ctactcatcc ttcatggctt
     6061 tgatgagcct gatgctccag atgagaaatt caattacagg cagaaccgac gtggatttct
     6121 tgatctcccc ggttaaaaat tctgatggca tcttttacga tagcaggaac tatgaagcgc
     6181 aagagaatgc gattctgcca aaaaatgcag acgccaacgg tgcctataac atcgccagga
     6241 aagtcctgtg ggcgatcggc cagttcaaaa aggccgaaga cgaaaaattg gacaaggtca
     6301 aaatcgctat cagcaacaaa gagtggctgg agtatgctca gacatccgta aagcataagc
     6361 gtcctgctgc caccaaaaag gccggacagg ctaagaaaaa gaagtgagac gactagtggc
     6421 ggccgccgac gtccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     6481 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     6541 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     6601 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     6661 gcgcggtgtc atctatgtta ctagatcggg aattgatccc ccctcgacag cttccggaaa
     6721 gggcgaattc gcaactttgt atacaaaagt tgccccatgg cgttccctct agagagataa
     6781 tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt
     6841 gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga ataatataat
     6901 ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg
     6961 gtctaaagga caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg
     7021 catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat
     7081 tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat
     7141 ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt
     7201 atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc
     7261 ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc
     7321 ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc
     7381 gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag
     7441 ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg
     7501 cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg
     7561 gggattcctt tcccmccgct ccttcgcttt cccttccycg cccgccgtaa taaatagaca
     7621 ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca
     7681 gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc
     7741 cccccccccc tctctmcctt ctctagatcg gcgttccggt ccatggttag ggcccggtag
     7801 ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg
     7861 ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt
     7921 ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt
     7981 ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt
     8041 gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt
     8101 ctggttgggc ggtcgttcta gatcggagta gaattaattc tgtttcaaac tacctggtgg
     8161 atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga
     8221 tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc
     8281 atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt
     8341 tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt
     8401 tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata
     8461 tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata catgatggca
     8521 tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta
     8581 tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg
     8641 tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc
     8701 gatgctcacc ctgttgtttg gtgttacttc tgcagggatc caaattactg atgagtccgt
     8761 gaggacgaaa cgagtaagct cgtctaattt ctactaagtg tagatgagac cggcgccgct
     8821 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     8881 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     8941 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     9001 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcc tccagctttt
     9061 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     9121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9241 ctttccaccg gtggtctctg gccggcatgg tcccagcctc ctcgctggcg ccggctgggc
     9301 aacatgcttc ggcatggcga atgggacggt acccggccgg aattcgaccc agctttcttg
     9361 tacaaagtgg ttcgataatt cttaattaac tagttctaga gcggccgcca ccgcggtgga
     9421 gctcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9481 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9541 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9601 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag
     9661 cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     9721 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     9781 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     9841 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     9901 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     9961 ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg acactctcgt
    10021 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
    10081 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
    10141 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
    10201 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
    10261 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
    10321 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
    10381 tatataagga agttcatttc atttggagag gacacgctga aatcaccagt ctctctctac
    10441 aaatctatct ctctcgagtc taccatgagc ccagaacgac gcccggccga catccgccgt
    10501 gccaccgagg cggacatgcc ggcggtctgc accatcgtca accactacat cgagacaagc
    10561 acggtcaact tccgtaccga gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt
    10621 ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc
    10681 tacgcgggcc cctggaaggc acgcaacgcc tacgactgga cggccgagtc gaccgtgtac
    10741 gtctcccccc gccaccagcg gacgggactg ggctccacgc tctacaccca cctgctgaag
    10801 tccctggagg cacagggctt caagagcgtg gtcgctgtca tcgggctgcc caacgacccg
    10861 agcgtgcgca tgcacgaggc gctcggatat gccccccgcg gcatgctgcg ggcggccggc
    10921 ttcaagcacg ggaactggca tgacgtgggt ttctggcagc tggacttcag cctgccggta
    10981 ccgccccgtc cggtcctgcc cgtcaccgag atttgactcg agtttctcca taataatgtg
    11041 tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca
    11101 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
    11161 attcctaaaa ccaaaatcca gtactaaaat ccagatcccc cgaattaatt cggcgttaat
    11221 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
    11281 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
    11341 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
    11401 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
    11461 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
    11521 gttgctgcct gtgatcaccg cggtttcaaa atcggctccg tcgatactat gttatacgcc
    11581 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
    11641 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
    11701 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
    11761 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
    11821 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
    11881 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
    11941 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
    12001 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
    12061 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
    12121 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
    12181 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
    12241 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
    12301 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
    12361 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
    12421 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
    12481 aaataaaata ttatatttta ctggatgaat tgttttagta cctagaatgc atgaccaaaa
    12541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    12601 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    12661 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    12721 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
    12781 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    12841 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    12901 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    12961 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    13021 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    13081 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    13141 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    13201 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    13261 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    13321 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    13381 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
    13441 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
    13501 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
    13561 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
    13621 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
    13681 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
    13741 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
    13801 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
    13861 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
    13921 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
    13981 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
    14041 tgtacgtgct atccacagga aacagacctt ttcgaccttt ttcccctgct agggcaattt
    14101 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
    14161 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
    14221 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
    14281 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
    14341 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
    14401 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
    14461 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
    14521 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
    14581 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
    14641 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
    14701 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
    14761 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
    14821 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
    14881 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
    14941 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
    15001 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
    15061 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
    15121 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
    15181 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
    15241 gcaggcaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
    15301 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
    15361 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
    15421 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
    15481 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
    15541 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
    15601 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
    15661 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
    15721 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
    15781 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
    15841 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
    15901 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
    15961 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
    16021 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
    16081 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    16141 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    16201 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    16261 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    16321 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    16381 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    16441 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
    16501 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
    16561 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
    16621 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
    16681 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
    16741 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
    16801 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
    16861 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
    16921 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
    16981 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
    17041 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
    17101 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
    17161 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
    17221 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta tt
//
