LOCUS       sequence-45369-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CmCherry-ABCG2a
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..28
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10..28
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    141..275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(178..197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     232..251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(604..623)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccagcgcgca attaaccctc actaaaggga acaaaagctg ggtaccgggc ccaatgcatt
       61 ggcgccgcgg ccgcgaatta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      121 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
      181 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      241 ccaaactcat caatgtatct tatcatgtct ggatctacgt aatacgactc actatagttc
      301 tagaggctcg agaggccttg aattcttact tgtacagctc gtccatgccg ccggtggagt
      361 ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
      421 tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt
      481 aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga
      541 tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca
      601 tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct
      661 tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt
      721 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt
      781 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca
      841 ggatgtcc
//
