LOCUS       sequence-453799-        4971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            emiRFP670 with SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4971)
  TITLE     Teusink Lab yeast expression plasmids
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-yemiRFP670-SpHis5
FEATURES             Location/Qualifiers
     source          1..4971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     329..348
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(567..671)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     739..757
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(795..817)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     917..936
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1017..1035
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1017..1034
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      2075..2262
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2130..2154)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            2337..3537
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5 
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        2337..2680
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2681..3334
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHTNQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMSNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      3340..3537
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3641..3660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3642..3660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3747..3764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3918..4506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3998..4017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4677..4971,1..497,498..566))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
       61 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
      121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
      181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
      241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
      301 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
      361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
      421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
      481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
      541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
      601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
      661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
      721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
      781 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
      841 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
      901 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatggacata
      961 ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
     1021 aggtgacact atagaacgcg gccgccagct gaagcttcgt acgctgcagg tcgacggatc
     1081 ggtgacggtg ctggtttaat taacatggct gaaggttctg ttgctagaca accagatttg
     1141 ttgacttgtg aacatgaaga aattcatttg gctggttcta ttcaaccaca tggtgctttg
     1201 ttggtcgttt ctgaacatga ccatagagtt attcaagcct ctgctaatgc cgctgaattc
     1261 ttaaacttgg gttctgtttt aggtgttcca ttagctgaaa ttgatggtga tttgttaatt
     1321 aagattttac cacatttaga tccaaccgct gaaggtatgc cagttgctgt tagatgtaga
     1381 atcggtaatc cttccactga atactgtggt ttgatgcata gaccaccaga aggtggtttg
     1441 attattgaat tggaaagagc tggtccatct attgatttat ccggtacttt ggctccagct
     1501 ttggaaagaa ttagaactgc tggttcttta agagctttgt gtgatgacac tgttttgtta
     1561 ttccaacaat gtactggtta tgatagagtt atggtttaca gattcgatga acaaggtcat
     1621 ggtttagttt tctctgaatg tcatgtccct ggtttggaat cttacttcgg taatagatac
     1681 ccatcctcta ctgttccaca aatggctaga caattatacg ttagacaaag agttagagtt
     1741 ttggtcgatg ttacttacca accagttcca ttggaaccaa gattatcccc attgactggt
     1801 agagatttag atatgtctgg ttgtttctta agatctatgt ctccttgtca tttgcaattc
     1861 ttgaaggata tgggtgttag agctactttg gccgtttctt tagttgttgg tggtaagttg
     1921 tggggtttgg ttgtttgtca ccattacttg ccaagattca ttagattcga attaagagct
     1981 atctgtaaga gattggctga aagaattgct actagaatta ccgctttaga atcttaagcg
     2041 gccgcctcga ggtcggcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt
     2101 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt
     2161 tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca
     2221 ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg ctagggataa
     2281 cagggtaata tagatctgtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca
     2341 tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg
     2401 actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt
     2461 ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc tgcgagcagg
     2521 gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata
     2581 taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc
     2641 taggatacag ttctcacatc acatccgaac ataaacaacc atgggtagga gggcttttgt
     2701 agaaagaaat acgaacgaaa cgaaaatcag cgttgccatc gctttggaca aagctccctt
     2761 acctgaagag tcgaatttta ttgatgaact tataacttcc aagcatacaa accaaaaggg
     2821 agaacaagta atccaagtag acacgggaat tggattcttg gatcacatgt atcatgcact
     2881 ggctaaacat gcaggctgga gcttacgact ttactcaaga ggtgatttaa tcatcgatga
     2941 tcatcacact gcagaagata ctgctattgc acttggtatt gcattcaagc aggctatgag
     3001 taactttgcc ggcgttaaaa gatttggaca tgcttattgt ccacttgacg aagctctttc
     3061 tagaagcgta gttgacttgt cgggacggcc ctatgctgtt atcgatttgg gattaaagcg
     3121 tgaaaaggtt ggggaattgt cctgtgaaat gatccctcac ttactatatt ccttttcggt
     3181 agcagctgga attactttgc atgttacctg cttatatggt agtaatgacc atcatcgtgc
     3241 tgaaagcgct tttaaatctc tggctgttgc catgcgcgcg gctactagtc ttactggaag
     3301 ttctgaagtc ccaagcacga agggagtgtt gtaaagagta ctgacaataa aaagattctt
     3361 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
     3421 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
     3481 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg
     3541 tcgattcgat actaacgccg ccatccagtt taaacgagct cgaattcatc gatgatatca
     3601 gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg agtcgtatta
     3661 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3721 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt g
//
