LOCUS       sequence-453878-        8443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  R2pH-LAMP1-labelled lysosomes with 3xLFA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Cheetham-Wilkinson IJ, Sivalingam B, Flitton C, Flottmann F, Vehling
            L, Drechsler M, Stojchevska M, Raimondi A, Paululat A, Frohlich F, 
            Swan LE, Stagi M
  TITLE     RpH-ILV: Probe for lysosomal pH and acute LLOMe-induced membrane 
            permeabilization in cell lines and Drosophila.
  JOURNAL   Sci Adv. 2025 Jan 3;11(1):eadr7325. doi: 10.1126/sciadv.adr7325. 
            Epub 2025 Jan 3.
  PUBMED    39752501
REFERENCE   2  (bases 1 to 8443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-01-3"; volume: "11(1):eadr7325. doi"; pages: " 
            10.1126/sciadv.adr7325. Epub 2025 Jan 3"
COMMENT     Sequence Label: R2pH-LAMPx3ALFA
FEATURES             Location/Qualifiers
     source          1..8443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..664
                     /label=chicken beta-actin promoter
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1689..1708
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1737..1746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1820..2527
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             2540..3247
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             3272..3292
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3293..4006
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3323..3351)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3946..3966
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4007..4027
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5183..5206
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5222..5260
                     /codon_start=1
                     /product="rationally designed epitope tag that forms a 
                     stable alpha-helix (Gotzke et al., 2019)"
                     /label=ALFA-tag
                     /note="It may be beneficial to flank the ALFA-tag sequence 
                     with proline residues to preserve the helical structure."
                     /translation="SRLEEELRRRLTE"
     CDS             5276..5314
                     /codon_start=1
                     /product="rationally designed epitope tag that forms a 
                     stable alpha-helix (Gotzke et al., 2019)"
                     /label=ALFA-tag
                     /note="It may be beneficial to flank the ALFA-tag sequence 
                     with proline residues to preserve the helical structure."
                     /translation="SRLEEELRRRLTE"
     CDS             5330..5368
                     /codon_start=1
                     /product="rationally designed epitope tag that forms a 
                     stable alpha-helix (Gotzke et al., 2019)"
                     /label=ALFA-tag
                     /note="It may be beneficial to flank the ALFA-tag sequence 
                     with proline residues to preserve the helical structure."
                     /translation="SRLEEELRRRLTE"
     primer_bind     complement(5461..5480)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5526..5581
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5580..5599)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5942..5958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5942..5958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5955..5977)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5966..5982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5990..5996,5997..6014,6015..6020))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6035..6056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6115..6310
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6161..6296
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6316..6450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6353..6372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6407..6426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6518..6535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6689..7277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6769..6788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7448..8239,8240..8308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8071..8090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8309..8413)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 ccccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      481 atgggggcgg gggggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcaccat
     1741 ggcggccccc ggcgcccggc ggccgctgct cctgctgctg ctggcaggcc ttgcacatgg
     1801 cgcctcagca gctagcggta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     1861 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     1921 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1981 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     2041 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     2101 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     2161 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     2221 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     2281 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     2341 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     2401 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     2461 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     2521 gtacaaggga gctagcggta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     2581 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     2641 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     2701 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     2761 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     2821 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     2881 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     2941 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     3001 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     3061 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     3121 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     3181 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     3241 gtacaaggga gctagcgggg ggcccgggat ggagaatctg tatttccagg ggatgagtaa
     3301 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     3361 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     3421 ccttaaattt atttgcacta ctggaaaact acctgttcct tggccaacac ttgtcactac
     3481 tttaacttat ggcgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt
     3541 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
     3601 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     3661 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     3721 caactataac gatcaccagg tgtacatcat ggcagacaaa caaaagaatg gaatcaaagc
     3781 taacttcaaa attagacaca acattgaaga tggaggcgtt caactagcag accattatca
     3841 acaaaatact ccaattggcg atgggcccgt ccttttacca gacaaccatt acctgtttac
     3901 aacttctact ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     3961 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaagaga atctgtattt
     4021 ccaggggccc ggggggctct ttgaggtgaa aaacaatggc acaacgtgta taatggccag
     4081 cttctctgcc tcctttctga ccacctacga gactgcgaat ggttctcaga tcgtgaacat
     4141 ttccctgcca gcctctgcag aagtactgaa aaatggcagt tcttgtggta aagaaaatgt
     4201 ttctgacccc agcctcacaa ttacttttgg aagaggatat ttactgacac tcaacttcac
     4261 aaaaaataca acacgttaca gtgtccagca tatgtatttt acatataact tgtcagatac
     4321 agaacatttt cccaatgcca tcagcaaaga gatctacacc atggattcca caactgacat
     4381 caaggcagac atcaacaaag cataccggtg tgtcagtgat atccgggtct acatgaagaa
     4441 tgtgaccgtt gtgctccggg atgccactat ccaggcctac ctgtcgagtg gcaacttcag
     4501 caaggaagag acacactgca cacaggatgg accttcccca accactgggc cacccagccc
     4561 ctcaccacca cttgtgccca caaaccccac tgtatccaag tacaatgtta ctggtaacaa
     4621 cggaacctgc ctgctggcct ctatggcact gcaactgaat atcacctacc tgaaaaagga
     4681 caacaagacg gtgaccagag cgttcaacat cagcccaaat gacacatcta gtgggagttg
     4741 cggtatcaac ttggtgaccc tgaaagtgga gaacaagaac agagccctgg aattgcagtt
     4801 tgggatgaat gccagctcta gcctgttttt cctgcaagga gtgcgcttga atatgactct
     4861 tcctgatgcc ctagtgccca cattcagcat ctccaaccat tcactgaaag ctcttcaggc
     4921 cactgtggga aactcataca agtgcaacac tgaggaacac atctttgtca gcaagatgct
     4981 ctccctcaat gtcttcagtg tgcaggtcca ggctttcaag gtggacagtg acaggtttgg
     5041 gtctgtggaa gagtgtgttc aggatggtaa caacatgttg atccccattg ctgtgggcgg
     5101 tgccctggca gggctggtcc tcatcgtcct cattgcctac ctcattggca ggaagaggag
     5161 tcacgccggc tatcagacca tcttggaggt tctgtttcaa gggcctgata tcgggggagg
     5221 ttcaagactc gaagaagagt tgaggcgacg gcttacggag gatatcggtg gtggatcccg
     5281 actggaagag gagctcagga gaaggttgac tgaggatatc ggagggggtt ctcggcttga
     5341 agaagaactt cgccgacgcc tcacggaaga tatctaggaa ttcactcctc aggtgcaggc
     5401 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     5461 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     5521 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     5581 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     5641 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     5701 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     5761 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     5821 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     5881 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     5941 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6001 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6061 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     6121 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6181 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6241 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6301 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6361 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6421 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     6481 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8401 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtc
//
