LOCUS       sequence-453896-        5151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            miRFP680 with URA3 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5151)
  TITLE     Teusink Lab yeast expression plasmids
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-ymiRFP680-URA3
FEATURES             Location/Qualifiers
     source          1..5151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(568..672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     740..758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(796..818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     918..937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1018..1036
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1018..1035
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      2094..2281
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2149..2173)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2356..2699
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3518..3715
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3822..3841)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3823..3841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3928..3945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4099..4687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4179..4198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4858..5151,1..498,499..567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
       61 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      961 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     1021 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     1081 cggtgacggt gctggtttaa ttaacatggc tgaaggttct gttgctagac aaccagattt
     1141 gttgacttgt gatgatgaac caattcatat tccaggtgct attcaaccac atggtttgtt
     1201 attagctttg gctgctgata tgaccattgt tgctggttct gataacttgc ctgaattgac
     1261 tggtttagct attggtgcct tgatcggtag atctgctgct gatgttttcg attctgaaac
     1321 ccataataga ttgactattg ctttggctga accaggtgct gctgtcggtg ctccaattac
     1381 cgtcggtttc accatgcgta aggatgccgg tttcattggt tcttggcata gacatgatca
     1441 attaattttc ttggaattgg aaccaccaca acgtgacgtt gccgaaccac aggctttctt
     1501 ccgtagaacc aactctgcta ttagaagatt acaagctgct gaaactttgg aatctgcttg
     1561 tgctgctgct gctcaagaag ttcgtaagat tactggtttc gatagagtta tgatctacag
     1621 atttgcttct gatttctctg gtgaagttat tgctgaagat agatgtgctg aagttgaatc
     1681 taagttgggt ttgcattacc cagcttctac tgttccagct caagctagaa gattgtacac
     1741 tattaatcca gttagaatta ttccagatat caactacaga ccagtcccag ttaccccaga
     1801 tttgaaccca gttactggta gacctattga tttatctttc gctattttga gatctgtttc
     1861 cccatgtcat ttggaattca tgagaaacat cggtatgcat ggtaccatgt ctatttctat
     1921 tttgagaggt gaaagattgt ggggtttgat tgtttgtcat catagaactc catactacgt
     1981 tgacttggat ggtagacaag cctgtaagag agttgctgaa agattggcta cccaaattgg
     2041 tgttatggaa gaataagcgg ccgcctcgag gtcggcgcgc cacttctaaa taagcgaatt
     2101 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
     2161 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
     2221 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
     2281 gcagatccgc tagggataac agggtaatat agatctgttt agcttgcctc gtccccgccg
     2341 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
     2401 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
     2461 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
     2521 ctgcggacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc
     2581 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata
     2641 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca
     2701 tgacagtcaa cactaagacc tatagtgaga gagcagaaac tcatgcctca ccagtagcac
     2761 aacgattatt tcgattaatg gaactgaaga aaaccaattt atgtgcatca attgatgttg
     2821 ataccactaa ggaattcctt gaattaattg ataaattggg tccttatgta tgcttaatca
     2881 agactcatat tgatataatc aatgattttt cctatgaatc cactattgaa ccattattag
     2941 aactttcacg taaacatcaa tttatgattt ttgaagatag aaaatttgct gatattggta
     3001 ataccgtgaa gaaacaatat attggtggag tttataaaat tagtagttgg gcagatatta
     3061 ctaatgctca tggtgtcact gggaatggag tagttgaagg attaaaacag ggagctaaag
     3121 aaaccaccac caaccaagag ccaagagggt tattgatgtt agctgaatta tcatcagtgg
     3181 gatcattagc atatggagaa tattctcaaa aaactgttga aattgctaaa tccgataagg
     3241 aatttgttat tggatttatt gcccaacgtg atatgggtgg acaagaagaa ggatttgatt
     3301 ggcttattat gacacctgga gttggattag atgataaagg tgatggatta ggacaacaat
     3361 atagaactgt tgatgaagtt gttagcactg gaactgatat tatcattgtt ggtagaggat
     3421 tgtttggtaa aggaagagat ccagatattg aaggtaaaag gtatagagat gctggttgga
     3481 atgcttattt gaaaaagact ggccaattat aatcagtact gacaataaaa agattcttgt
     3541 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa
     3601 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag
     3661 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc
     3721 gattcgatac taacgccgcc atccagtgtc gaaaacgagc tcgaattcat cgatgatatc
     3781 agatccacta gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt
     3841 aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3901 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3961 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4021 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4081 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4141 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4201 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4261 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4321 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4381 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4441 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4501 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4561 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4621 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4681 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4741 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4801 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4861 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4921 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4981 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5041 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5101 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt t
//
