LOCUS       sequence-453943-        9492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA 
            polymerase D219A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9492)
  TITLE     Escherichia phage T4 Expression Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-Impact-T4gp43(exo-)
FEATURES             Location/Qualifiers
     source          1..9492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(559..1374)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(559..1374)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1282..1301
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1366..1385
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1496..2084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1985..2004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2238..2255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2270..2409
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2335..2354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2495..2517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2511..2702)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3511..4593)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4555..4574
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4594..4671)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4877..4896
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4980..4999
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4980..4998
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4999..5023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5038..5060
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7764..8582
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             9038..9049
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             9159..9314
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             9336..9353
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9406..9424)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9420..9467
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      601 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      661 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      721 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      781 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
      841 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      901 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      961 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     1021 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     1081 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     1141 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     1201 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     1261 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     1321 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     1381 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     1441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc gggggagcgc ctgatgcggt
     2281 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     2341 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2401 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2521 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     2581 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     2641 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     2701 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     2761 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     2821 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     2881 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     2941 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     3001 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3061 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     3121 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     3181 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     3241 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     3301 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     3361 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     3421 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     3481 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3541 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3601 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     3661 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     3721 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     3781 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     3841 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     3901 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     3961 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     4021 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     4081 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     4141 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     4201 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     4261 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     4321 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     4381 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     4441 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     4501 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     4561 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     4621 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     4681 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     4741 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     4801 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     4861 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     4921 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
     4981 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     5041 gtttaacttt aagaaggaga tatacataga tgaaagaatt ttatatctct attgaaacag
     5101 tcggaaataa cattgttgaa cgttatattg atgaaaatgg aaaggaacgt acccgtgaag
     5161 tagaatatct tccaactatg tttaggcatt gtaaggaaga gtcaaaatac aaagacatct
     5221 atggtaaaaa ctgcgctcct caaaaatttc catcaatgaa agatgctcga gattggatga
     5281 agcgaatgga agacatcggt ctcgaagctc tcggtatgaa cgattttaaa ctcgcttata
     5341 taagtgatac atatggttca gaaattgttt atgaccgaaa atttgttcgt gtagctaact
     5401 gtgacattga ggttactggt gataaatttc ctgacccaat gaaagcagaa tatgaaattg
     5461 atgctatcac tcattacgat tcaattgacg atcgttttta tgttttcgac cttttgaatt
     5521 caatgtacgg ttcagtatca aaatgggatg caaagttagc tgctaagctt gactgtgaag
     5581 gtggtgatga agttcctcaa gaaattcttg accgagtaat ttatatgcca ttcgataatg
     5641 agcgtgatat gctcatggaa tatatcaatc tttgggaaca gaaacgacct gctattttta
     5701 ctggttggaa tattgagggg tttgccgttc cgtatatcat gaatcgtgtt aaaatgattc
     5761 tgggtgaacg tagtatgaaa cgtttctctc caatcggtcg ggtaaaatct aaactaattc
     5821 aaaatatgta cggtagcaaa gaaatttatt ctattgatgg cgtatctatt cttgattatt
     5881 tagatttgta caagaaattc gcttttacta atttgccgtc attctctttg gaatcagttg
     5941 ctcaacatga aaccaaaaaa ggtaaattac catacgacgg tcctattaat aaacttcgtg
     6001 agactaatca tcaacgatac attagttata acatcattga cgtagaatca gttcaagcaa
     6061 tcgataaaat tcgtgggttt atagatctgg ttttaagtat gtcttattac gctaaaatgc
     6121 ctttttctgg tgtaatgagt cctattaaaa cttgggatgc tattattttt aactcattga
     6181 aaggtgaaca taaggttatt cctcaacaag gttcgcacgt taaacagagt tttccgggtg
     6241 catttgtgtt tgaacctaaa ccaattgcac gtcgatacat tatgagtttt gacttgacgt
     6301 ctctgtatcc gagcattatt cgccaggtta acattagtcc tgaaactatt cgtggtcagt
     6361 ttaaagttca tccaattcat gaatatatcg caggaacagc tcctaaaccg agtgatgaat
     6421 attcttgttc tccgaatgga tggatgtatg ataaacatca agaaggtatc attccaaagg
     6481 aaatcgctaa agtatttttc cagcgtaaag actggaaaaa gaaaatgttc gctgaagaaa
     6541 tgaatgccga agctattaaa aagattatta tgaaaggcgc agggtcttgt tcaactaaac
     6601 cagaagttga acgatatgtt aagttcagtg atgatttctt aaatgaacta tcgaattaca
     6661 ccgaatctgt tctcaatagt ctgattgaag aatgtgaaaa agcagctaca cttgctaata
     6721 caaatcagct gaaccgtaaa attctcatta acagtcttta tggtgctctt ggtaatattc
     6781 atttccgtta ctatgatttg cgaaatgcta ctgctatcac aattttcggc caagtcggta
     6841 ttcagtggat tgctcgtaaa attaatgaat atctgaataa agtatgcgga actaatgatg
     6901 aagatttcat tgcagcaggt gatactgatt cggtatatgt ttgcgtagat aaagttattg
     6961 aaaaagttgg tcttgaccga ttcaaagagc agaacgattt ggttgaattc atgaatcagt
     7021 tcggtaagaa aaagatggaa cctatgattg atgttgcata tcgtgagtta tgtgattata
     7081 tgaataaccg cgagcatctg atgcatatgg accgtgaagc tatttcttgc cctccgcttg
     7141 gttcaaaggg cgttggtgga ttttggaaag cgaaaaagcg ttatgctctg aacgtttatg
     7201 atatggaaga taagcgattt gctgaaccgc atctaaaaat catgggtatg gaaactcagc
     7261 agagttcaac accaaaagca gtgcaagaag ctctcgaaga aagtattcgt cgtattcttc
     7321 aggaaggtga agagtctgtc caagaatact acaagaactt cgagaaagaa tatcgtcaac
     7381 ttgactataa agttattgct gaagtaaaaa ctgcgaacga tatagcgaaa tatgatgata
     7441 aaggttggcc aggatttaaa tgcccgttcc atattcgtgg tgtgctaact tatcgtcgag
     7501 ctgttagcgg tttaggtgta gctccaattt tggatggaaa taaagtaatg gttcttccat
     7561 tacgtgaagg aaatccattt ggtgacaagt gcattgcttg gccatcgggt acagaacttc
     7621 caaaagaaat tcgttctgat gtgctatctt ggattgacca ctcaactttg ttccaaaaat
     7681 cgtttgttaa accgcttgcg ggtatgtgtg aatcggctgg catggactat gaagaaaaag
     7741 cttcgttaga cttcctgttt ggctgctttg ccaagggtac caatgtttta atggcggatg
     7801 ggtctattga atgtattgaa aacattgagg ttggtaataa ggtcatgggt aaagatggca
     7861 gacctcgtga ggtaattaaa ttgcccagag gaagagaaac tatgtacagc gtcgtgcaga
     7921 aaagtcagca cagagcccac aaaagtgact caagtcgtga agtgccagaa ttactcaagt
     7981 ttacgtgtaa tgcgacccat gagttggttg ttagaacacc tcgtagtgtc cgccgtttgt
     8041 ctcgtaccat taagggtgtc gaatattttg aagttattac ttttgagatg ggccaaaaga
     8101 aagcccccga cggtagaatt gttgagcttg tcaaggaagt ttcaaagagc tacccaatat
     8161 ctgaggggcc tgagagagcc aacgaattag tagaatccta tagaaaggct tcaaataaag
     8221 cttattttga gtggactatt gaggccagag atctttctct gttgggttcc catgttcgta
     8281 aagctaccta ccagacttac gctccaattc tttatgagaa tgaccacttt ttcgactaca
     8341 tgcaaaaaag taagtttcat ctcaccattg aaggtccaaa agtacttgct tatttacttg
     8401 gtttatggat tggtgatgga ttgtctgaca gggcaacttt ttcggttgat tccagagata
     8461 cttctttgat ggaacgtgtt actgaatatg ctgaaaagtt gaatttgtgc gccgagtata
     8521 aggacagaaa agaaccacaa gttgccaaaa ctgttaattt gtactctaaa gttgtcagag
     8581 gtaatggtat tcgcaataat cttaatactg agaatccatt atgggacgct attgttggct
     8641 taggattctt gaaggacggt gtcaaaaata ttccttcttt cttgtctacg gacaatatcg
     8701 gtactcgtga aacatttctt gctggtataa ttgattctga tggctatgtt actgatgagc
     8761 atggtattaa agcaacaata aagacaattc atacttctgt cagagatggt ttggtttccc
     8821 ttgctcgttc tttaggctta gtagtctcgg ttaacgcaga acctgctaag gttgacatga
     8881 atgtcaccaa acataaaatt agttatgcta tttatatgtc tggtggagat gttttgctta
     8941 acgttctttc gaagtgtgcc ggctctaaaa aattcaggcc tgctcccgcc gctgcttttg
     9001 cacgtgagtg ccgcggattt tatttcgagt tacaagaatt gaaggaagac gattattatg
     9061 ggattacttt atctgatgat tctgatcatc agtttttgct tggatcccag gttgtcgtcc
     9121 atgcatgcgg tggcctgacc ggtctgaact caggcctcac gacaaatcct ggtgtatccg
     9181 cttggcaggt caacacagct tatactgcgg gacaattggt cacatataac ggcaagacgt
     9241 ataaatgttt gcagccccac acctccttgg caggatggga accatccaac gttcctgcct
     9301 tgtggcagct tcaatgataa gcggccgcac tcgagcacca ccaccaccac cactgagatc
     9361 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     9421 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
     9481 ctatatccgg at
//
