LOCUS       sequence-453968-        9343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a chimeric construct having N-terminal human EGFR and 
            C-terminal human Tyro3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Kimani SG, Kumar S, Davra V, Chang YJ, Kasikara C, Geng K, Tsou WI, 
            Wang S, Hoque M, Bohac A, Lewis-Antes A, De Lorenzo MS, Kotenko SV, 
            Birge RB
  TITLE     Normalization of TAM post-receptor signaling reveals a cell invasive
            signature for Axl tyrosine kinase.
  JOURNAL   Cell Commun Signal. 2016 Sep 6;14(1):19. doi: 
            10.1186/s12964-016-0142-1.
  PUBMED    27595981
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2016-09-6"; volume: "14(1):19. doi"; pages: " 
            10.1186/s12964-016-0142-1"
COMMENT     Sequence Label: pEF2-EFGR-hTyro
FEATURES             Location/Qualifiers
     source          1..9343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1420..1438)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1421..1438)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1458..1475)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1464..1688
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1734..2162
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1821..1840)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2031..2052
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2171..2191)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2176..2505
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2356..2491
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2418..2437
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2572..3366
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2626..2645)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3236..3255
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3540..3661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3577..3596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3631..3650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3710..3726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3710..3726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3723..3745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3734..3750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3758..3764,3765..3782,3783..3788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3803..3824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3941..3958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4112..4700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4192..4211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4871..5662,5663..5731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5494..5513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5732..5836)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5911..5930)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6218..7396
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6449..7387
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7344..7364
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 atcccgtcct ggctagcagt ggtccttggt gtgctaacgg ccctggtgac ggctgctgcc
       61 ctggccctca tcctgcttcg aaagagacgg aaagagacgc ggtttgggca agcctttgac
      121 agtgtcatgg cccggggaga gccagccgtt cacttccggg cagcccggtc cttcaatcga
      181 gaaaggcccg agcgcatcga ggccacattg gacagcttgg gcatcagcga tgaactaaag
      241 gaaaaactgg aggatgtgct catcccagag cagcagttca ccctgggccg gatgttgggc
      301 aaaggagagt ttggttcagt gcgggaggcc cagctgaagc aagaggatgg ctcctttgtg
      361 aaagtggctg tgaagatgct gaaagctgac atcattgcct caagcgacat tgaagagttc
      421 ctcagggaag cagcttgcat gaaggagttt gaccatccac acgtggccaa acttgttggg
      481 gtaagcctcc ggagcagggc taaaggccgt ctccccatcc ccatggtcat cttgcccttc
      541 atgaagcatg gggacctgca tgccttcctg ctcgcctccc ggattgggga gaaccccttt
      601 aacctacccc tccagaccct gatccggttc atggtggaca ttgcctgcgg catggagtac
      661 ctgagctctc ggaacttcat ccaccgagac ctggctgctc ggaattgcat gctggcagag
      721 gacatgacag tgtgtgtggc tgacttcgga ctctcccgga agatctacag tggggactac
      781 tatcgtcaag gctgtgcctc caaactgcct gtcaagtggc tggccctgga gagcctggcc
      841 gacaacctgt atactgtgca gagtgacgtg tgggcgttcg gggtgaccat gtgggagatc
      901 atgacacgtg ggcagacgcc atatgctggc atcgaaaacg ctgagattta caactacctc
      961 attggcggga accgcctgaa acagcctccg gagtgtatgg aggacgtgta tgatctcatg
     1021 taccagtgct ggagtgctga ccccaagcag cgcccgagct ttacttgtct gcgaatggaa
     1081 ctggagaaca tcttgggcca gctgtctgtg ctatctgcca gccaggaccc cttatacatc
     1141 aacatcgaga gagctgagga gcccactgcg ggaggcagcc tggagctacc tggcagggat
     1201 cagccctaca gtggggctgg ggatggcagt ggcatggggg cagtgggtgg cactcccagt
     1261 gactgtcggt acatactcac ccccggaggg ctggctgagc agccagggca ggcagagcac
     1321 cagccagaga gtcccctcaa tgagacacag aggcttttgc tgctgcagca agggctactg
     1381 ccacacagta gctgttgaat tcctactaga gggccctatt ctatagtgtc acctaaatgc
     1441 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     1501 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     1561 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     1621 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     1681 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     1741 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1801 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1861 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1921 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     1981 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2041 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2101 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2161 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2221 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2281 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     2341 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2401 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     2461 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     2521 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     2581 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     2641 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     2701 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     2761 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     2821 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     2881 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     2941 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3001 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3061 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     3121 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3181 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3241 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3301 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     3361 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     3421 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     3481 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     3541 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3601 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3661 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     3721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     3901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5401 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5461 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5521 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5581 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5641 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5701 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5761 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5821 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     5881 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5941 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     6001 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     6061 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     6121 gcttttgcaa aaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
     6181 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     6241 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     6301 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     6361 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     6421 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     6481 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     6541 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     6601 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     6661 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     6721 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     6781 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     6841 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     6901 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     6961 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     7021 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     7081 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     7141 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     7201 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     7261 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     7321 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     7381 atttcaggtg tcgtgaggaa ttagcttggt accagcgatg cgaccctccg ggacggccgg
     7441 ggcagcgctc ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa
     7501 gaaagtttgc caaggcacga gtaacaagct cacgcagttg ggcacttttg aagatcattt
     7561 tctcagcctc cagaggatgt tcaataactg tgaggtggtc cttgggaatt tggaaattac
     7621 ctatgtgcag aggaattatg atctttcctt cttaaagacc atccaggagg tggctggtta
     7681 tgtcctcatt gccctcaaca cagtggagcg aattcctttg gaaaacctgc agatcatcag
     7741 aggaaatatg tactacgaaa attcctatgc cttagcagtc ttatctaact atgatgcaaa
     7801 taaaaccgga ctgaaggagc tgcccatgag aaatttacag gaaatcctgc atggcgccgt
     7861 gcggttcagc aacaaccctg ccctgtgcaa cgtggagagc atccagtggc gggacatagt
     7921 cagcagtgac tttctcagca acatgtcgat ggacttccag aaccacctgg gcagctgcca
     7981 aaagtgtgat ccaagctgtc ccaatgggag ctgctggggt gcaggagagg agaactgcca
     8041 gaaactgacc aaaatcatct gtgcccagca gtgctccggg cgctgccgtg gcaagtcccc
     8101 cagtgactgc tgccacaacc agtgtgctgc aggctgcaca ggcccccggg agagcgactg
     8161 cctggtctgc cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc ccccactcat
     8221 gctctacaac cccaccacgt accagatgga tgtgaacccc gagggcaaat acagctttgg
     8281 tgccacctgc gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg gctcgtgcgt
     8341 ccgagcctgt ggggccgaca gctatgagat ggaggaagac ggcgtccgca agtgtaagaa
     8401 gtgcgaaggg ccttgccgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc
     8461 actctccata aatgctagga atattaaaca cttcaaaaac tgcacctcca tcagtggcga
     8521 tctccacatc ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga
     8581 tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca
     8641 ggcttggcct gaaaacagga cggaccttca tgcctttgag aacctagaaa tcatacgcgg
     8701 caggaccaag caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt
     8761 gggattacgc tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa
     8821 tttgtgctat gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac
     8881 caaaattata agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc
     8941 cttgtgctcc cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa
     9001 tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga
     9061 gtttgtggag aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa
     9121 catcacctgc acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg
     9181 cccccactgc gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg
     9241 gaagtacgca gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg
     9301 cactgggcca ggtcttgaag gctgtccaac gaatgggcct aag
//
