LOCUS       sequence-454002-        9779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and 
            with constitutive RFP expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Gould SI, Wuest AN, Dong K, Johnson GA, Hsu A, Narendra VK, Atwa O, 
            Levine SS, Liu DR, Sanchez Rivera FJ
  TITLE     High-throughput evaluation of genetic variants with prime editing 
            sensor libraries.
  JOURNAL   Nat Biotechnol. 2024 Mar 12. doi: 10.1038/s41587-024-02172-9.
  PUBMED    38472508
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Mar 12. doi: 10.1038/s41587-024-02172-9."
COMMENT     Sequence Label: Lenti-UPEmS-tevo
FEATURES             Location/Qualifiers
     source          1..9779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(72..863,864..932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     695..714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(933..1037)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1112..1131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1303..1682
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1684..1882
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1837..1857
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1900..2080
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2127..2252
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2745..2978
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3163..3207
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3505..3622
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3718..3966
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3718..3738
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3889..3908
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     terminator      complement(3976..4007)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(4024..4701)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             4708..4719
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    4737..4755
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4762..4780
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4887..5098
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5152..5847
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(5285..5303)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5553..5572
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5936..6524
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5989..6009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6407..6418)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6596..6829
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6855..6872)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6861..7085
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7131..7559
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7218..7237)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7428..7449
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7568..7588)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7573..7902
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7753..7888
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7815..7834
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7950..7997
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8016..8390
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8520..8641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8557..8576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8611..8630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8690..8706)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8690..8706)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8703..8725)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8714..8730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8738..8744,8745..8762,8763..8768))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8783..8804
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8921..8938)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9092..9680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9172..9191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
       61 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      121 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      181 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      241 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      301 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      361 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      421 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      481 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      541 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      601 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      661 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      721 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      781 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      841 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      901 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1021 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     1081 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     1141 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     1201 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     1261 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     1321 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     1381 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     1441 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     1501 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     1561 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     1621 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1681 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     1741 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1801 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     1861 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
     1921 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     1981 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     2041 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     2101 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     2161 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     2221 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     2281 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     2341 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     2401 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     2461 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     2521 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     2581 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     2641 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     2701 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     2761 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     2821 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     2881 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     2941 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     3001 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     3061 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     3121 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     3181 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     3241 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     3301 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     3361 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     3421 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     3481 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     3541 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     3601 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     3661 aattaaggta ccgatcagtg tgagggagtg taaagctggt ttaaggtcgg gcaggaagag
     3721 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     3781 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     3841 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     3901 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     3961 aacacccgag acggtagtca aaagcctccg gtcggaggct tttgacttgg ctgaggagtg
     4021 ccattaagca ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta
     4081 gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg
     4141 aaccggtttt ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt
     4201 cagtttcaga cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc
     4261 ggtggaagct tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt
     4321 ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt
     4381 aacggtaaca acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg
     4441 gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc
     4501 gtactggaac tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac
     4561 tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac cttcgatttc
     4621 gaactcgtga ccgttaacgg aaccttccat acgaactttg aaacgcatga actctttgat
     4681 aacgtcttcg ctactcgcca tagatccttt ctcctctttc agatccgtgc tcagtatctc
     4741 tatcactgat agggatgtca atctctatca ctgataggga cgtctcgcgc ggttctatct
     4801 agttacgcgt taaaccaact agaatttttt taaaccgctg ttcctaggaa tcccgaggcc
     4861 ttcgagtggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     4921 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     4981 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5041 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     5101 gtcgtgacgc gggacccgac attagcgcta cagcttaagc ggtcgaccac catggtgagc
     5161 aagggcgagg cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg
     5221 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     5281 accgccaagc tgaaggtgac caagggtggc cccctgccct tctcctggga catcctgtcc
     5341 cctcagttca tgtacggctc cagggccttc accaagcacc ccgccgacat ccccgactac
     5401 tataagcagt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     5461 ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg
     5521 aagctccgcg gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc
     5581 tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg cgacattaag
     5641 atggccctgc gcctgaagga cggcggccgc tacctggcgg acttcaagac cacctacaag
     5701 gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt ggacatcacc
     5761 tcccacaacg aggactacac cgtggtggaa cagtacgaac gctccgaggg ccgccactcc
     5821 accggcggca tggacgagct gtacaagtaa aaacgttgtt gtttggggtt gaattactct
     5881 acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta tcgataatca
     5941 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6001 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6061 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6121 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6181 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6241 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6301 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6361 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6421 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6481 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     6541 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6601 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6661 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6721 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6781 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccggcga
     6841 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6901 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6961 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     7021 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     7081 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     7141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7501 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     7561 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7621 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7681 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7741 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7801 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     7861 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     7921 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     7981 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     8041 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     8101 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     8161 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     8221 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     8281 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     8341 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     8401 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     8461 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     8521 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8581 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8641 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8701 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8761 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8821 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8881 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8941 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9001 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9061 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9121 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9181 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9241 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9301 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9361 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9421 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9481 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9541 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9601 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9661 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9721 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacc
//
