LOCUS       sequence-45401-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6652 MSCV-P C-Flag HA 8E7-Kozak
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..84
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     100..116
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     144..163
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        144..162
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    192..216
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(526..550)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             582..605
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             621..647
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             621..647
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(695..718)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tcgggtagcc cttgtcaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc
       61 ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct
      121 tctctaggcg ccggaattag atctaatacg actcactata gggagaccca agctggctag
      181 ttaagctatc aacaagtttg tacaaaaaag ttggcaccat gattggtaaa gaggtcactg
      241 tgcaagattt tgtgttggag ttaagtgaga tacagcctga agtgttacca gttgacctgc
      301 tttgtgaaga ggaattacca aacgaacagg aaacggagga ggagctagac atcgaaagaa
      361 ctgtattcaa aattgttgca ccgtgtggct gcagctgctg tcaggtcaag ctacgtcttt
      421 ttgtcaacgc aactgattcg ggtatcagga cctttcaaga attgctgttc agagacctac
      481 agcttctgtg tcctgagtgc cgcggtaact gcaaacatgg cggatcacca actttcttgt
      541 acaaagtggt tgatctagag ggcccgcggt tcgaactcga ggactacaag gacgacgatg
      601 acaagtcggc cgctggagga tacccctacg acgtgcccga ctacgcctag aattctaccg
      661 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
      721 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
      781 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
      841 gaagttcccc cccgccccgc actcgcgctg gaga
//
