LOCUS       sequence-454028-       10763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes constitutive rtTA, tetOn-Pcdh19-GFP, and constitutive 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   McNamara HM, Solley SC, Adamson B, Chan MM, Toettcher JE
  TITLE     Recording morphogen signals reveals mechanisms underlying gastruloid
            symmetry breaking.
  JOURNAL   Nat Cell Biol. 2024 Oct 2. doi: 10.1038/s41556-024-01521-9.
  PUBMED    39358450
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2024 Oct 2. doi: 10.1038/s41556-024-01521-9."
COMMENT     Sequence Label: pHM148
FEATURES             Location/Qualifiers
     source          1..10763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     759..776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    893..914
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(929..934,935..952,953..959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     972..994
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     991..1007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     991..1007
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   1042..1076
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1451..1950
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1486..1509)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1883..1902
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1974..2717
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    2890..3011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3107..3485
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3115..3133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3151..3169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3187..3205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3223..3241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3259..3277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3295..3313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3331..3349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3394..3418
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3486..3495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6825..6827,6828..6830,6831..7544)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6825..6827,6828..6830,6831..7541)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6870..6891)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7131..7150)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7478..7499
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7589..7710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7907..7927)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7912..8241
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8092..8227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8251..8961
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8396..8414)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8664..8683
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     repeat_region   9205..9267
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9379..9398)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9498..9520
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9558..9576)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9644..9748
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9749..9817,9818..10609)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9967..9986)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gctggcacta caggtttccc gactggaaag cgggcagtga tcgcaacgca attaatgtga
      901 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      961 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgccg
     1021 gggcgcgcca ttctagatta accctagaaa gatagtctgc gtaaaattga cgcatgcatt
     1081 cttgaaatat tgctctctct ttctaaatag cgcgaatccg tcgctgtgca tttaggacat
     1141 ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat
     1201 tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta cgtgactttt
     1261 aagatttaac tcatacgata attatattgt tatttcatgt tctacttacg tgataactta
     1321 ttatatatat attttcttgt tatagatatc aactagaatg ctagcatggg cccagatctc
     1381 cgaggggacc cgacaggccc gcagggacag cagagatcca gtttggatcg ataagcttga
     1441 tatcgaattg gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     1501 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     1561 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     1621 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     1681 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     1741 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     1801 ctgggaaggg gtgggtcccg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     1861 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     1921 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgatgagcc
     1981 ggctggacaa gagcaaagtg atcaacagcg ccctggaact gctgaacggc gtgggcatcg
     2041 agggcctgac cacccggaag ctggcccaga aactgggcgt ggaacagccc accctgtact
     2101 ggcacgtgaa gaacaagcgg gccctgctgg acgccctgcc catcgagatg ctggaccggc
     2161 accacaccca cagctgccct ctggaaggcg agagctggca ggacttcctg cggaacaacg
     2221 ccaagagcta cagatgcgcc ctgctgagcc accgggacgg cgccaaagtg cacctgggca
     2281 ccagacccac cgagaagcag tacgagacac tggaaaacca gctggccttc ctgtgccagc
     2341 agggcttcag cctggaaaac gccctgtacg ccctgagcgc cgtgggccac ttcaccctgg
     2401 gctgcgtgct ggaagaacag gaacaccagg tcgccaaaga ggaaagagag acacccacca
     2461 ccgacagcat gccccccctg ctgaagcagg ccatcgagct gttcgacaga cagggcgccg
     2521 agcccgcctt cctgttcggc ctggaactga tcatctgcgg cctcgagaag cagctgaagt
     2581 gcgagagcgg cggacccacc gacgccctgg acgacttcga cctggacatg ctgcccgccg
     2641 atgccctgga tgattttgat ctggatatgc tccctgctga cgctctcgat gacttcgatc
     2701 tcgatatgct gccaggcaag cttagccatg gcttcccgcc ggaggtggag gagcaggatg
     2761 atggcacgct gcccatgtct tgtgcccagg agagcgggat ggaccgtcac cctgcagcct
     2821 gtgcttctgc taggatcaat gtgtgataat tctagagtcg gggcggccgg ccgcttcgag
     2881 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     2941 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3001 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     3061 gggaggtttt ttaaagcaag cgatcacgag actagcctcg agagttggct ttactcccta
     3121 tcagtgatag agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga
     3181 ctttactccc tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag
     3241 aacgtatgac cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta
     3301 tcagtgatag agaacgtata tccagtttac tccctatcag tgatagagaa cgtataagct
     3361 ttaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
     3421 ctggagcaat tccacaacac ttttgtctta taccaacttt ccgtaccact tcctaccctc
     3481 gtaaagccac catggagtct ctcctgctgc cggtgctatt gctgctggct gtactgtgga
     3541 cgcaggcggc tgccctgatt aaccttaaat actcggtcga agaggagcag cgcgccggga
     3601 cggtgatcgc taacgtggcc aaggacgcgc gggaggccgg cttcgcgctg gacccccgac
     3661 aggcttctgc tttccgcgtg gtgtccaact cggctccgca cctggtggac atcaacccca
     3721 gctcgggcct cttggtcacc aagcagaaga ttgaccgaga cctgctgtgc cgccagagcc
     3781 ccaagtgtat catctcgctc gaggtcatgt ccagctcaat ggagatctgt gtgattaagg
     3841 tggagatcaa agacctgaac gacaatgctc ccagttttcc ggccgcacag atagagctgg
     3901 agatctctga ggcagccagc cccggcaccc gcatcccact ggacagtgca tatgacccgg
     3961 actccggcag ctttggtgtg cagacctacg agctcacgcc caacgagctg tttggcctgg
     4021 aaataaagac gcggggtgac ggttcgcgct ttgctgaact ggtggtggag aagagcctgg
     4081 accgtgagac acagtcacat tacagctttc gcattacggc tctcgacgga ggcgacccac
     4141 cacacatggg cacagttggc ctcagcatca aggtgaccga ttcgaatgac aacaacccag
     4201 tgtttggcga gtccacctac tcagtgagcg tgcctgaaaa ttcacctccc aatacacccg
     4261 tcatccgcct caatgccagc gatccagatg agggcaccaa tggccaagtg gtctattcct
     4321 tctatggtta tgtcaatgac cgtacgcgtg aactcttcca aattgaccca cacagcggcc
     4381 tggtcaccgt caccggtgcg ctagactatg aagagggcca tgtgtacgaa ctggatgtgc
     4441 aagccaaaga cctggggccc aactctatcc ctgcacactg caaagtcaca gtcagcgtac
     4501 ttgatactaa tgacaacccg ccaattatca acctcctgtc ggtcaatagc gagcttgtgg
     4561 aggtgagcga gagcgccccc ccgggctatg tgattgccct ggttcgggtg tctgatcgcg
     4621 actccgggct caatggacgt gtgcagtgcc gcttgctggg caatgtcccc tttcgactgc
     4681 aggagtatga gagcttctcc actattcttg tggatgggag gctggaccga gagcagcatg
     4741 accagtacaa tctcactatt caggctcgag acagcggtgt gcctatgctg cagagtgcca
     4801 agtcgttcac tgtgcgtatc acagatgaga atgacaacca cccacacttc tccaagcctt
     4861 actaccaagt cattgtacag gagaacaaca ctcctggtgc ctatctgctc tcagtgtctg
     4921 cccgcgaccc tgacatgggt cttaatggca gtgtctccta tcaaattgtg ccatcacagg
     4981 tgcgagacat gccagtcttc acctatgtct caatcaatcc caactctggt gacatctatg
     5041 cgctccgttc ctttaaccat gagcagacca aggcgtttga attcaaggtc ctggccaagg
     5101 atggcggctt gccctccctg caaagcaatg ccactgtgag ggtcattatc ctcgacgtca
     5161 atgacaatac accggtcatc actgccccac ctctgatcaa tggcactgct gaggtctaca
     5221 ttcctcgaaa ctctggcata ggctatttgg tgactgtagt taaggcagac gattatgatg
     5281 agggcgaaaa tggccgagtc acctatgaca tgacagaagg tgaccgaggt ttcttcgaaa
     5341 tagaccaggt caatggagaa gtcagaacca ctcgtacctt taatgagaac tccaagcctt
     5401 cctatgagct tatagtggtg gcccatgacc atggcaagac atctctctct gcttctgccc
     5461 ttgtcctaat atacctgtcc ccagctcttg atgcccaaga gtcaatgggc tctgtgaact
     5521 tatccctgat tttcattatt gctctgggct ccattgcggg cattctcttt gtcactatga
     5581 tcttcgtggc aatcaaatgc aagcgtgaca acaaagagat ccggacctac aattgcagaa
     5641 tcgctgagta ctcctatggg catcaaaaga aatcaagtaa gaagaaaaaa attagtaaga
     5701 atgacatccg ccttgtacct cgggacgtgg aggagacaga caagatgaat gttgtcagtt
     5761 gctcctccct tacttcgtcc ctcaactatt tcgactacca ccagcagaca ctgcccctgg
     5821 gctgtcgtcg ctctgagagc actttcctga atgtggagaa ccagaatacc cgtaacacca
     5881 ctgccagcca catctaccat cactccttca acagccaggg cccacagcag ccagacctga
     5941 tcatcaatgg tgtgcccctg cctgagactg aaaactattc ttttgactct aactacgtga
     6001 atagccgtgc ccatttaatc aaaagctcca ccttcaagga cctagagggc aacagcctga
     6061 aggatagtgg gcatgaggag agtgaccaga ctgacagtga gcatgatgtc cagaggagcc
     6121 tgtactgcga tactgctgtg aatgatgtcc tgaacaccag tgtgacttcc atgggatctc
     6181 agatgcctga ccatgaccag aatgaaggat ttcattgcag ggaagaatgc cggattcttg
     6241 gccactctga tagatgctgg atgcctcgga atcccatgcc cacccgctcc aagtcacctg
     6301 agcacgtgag gaacatcatc gctctgtcca ttgaagctac tgctgctgat gttgaggctt
     6361 atgacgactg cggccccacc aagcggactt tcgcgacctt tggaaaagat gtcagtagcc
     6421 accgggctga ggagcgtccc atcctgaaag gcaagcggac tgtcgatgtg accatctgca
     6481 gccccaaagt caacagcgct atccgggagg caggcaatgg gtgtgaagct attagccctg
     6541 tcacctcccc actccacctg aagagccctc tgcccaccaa gccttctata tcttacaccg
     6601 tcgccctggc tcctccagct cacgatctgg agcaccatgc caacagtggc gcctctcgtc
     6661 cttctgaggc tgagccccgt ggagccgaca acgagaaagt gatgcatgag gtcaacccta
     6721 ttcggaagga tggtcgtgac aaggaatctc ctagcgtgaa gcgtctgaag gatatcgttc
     6781 tcctggaggc ctccccctcc aaccccggcg cctccaacgg ctccatggtg agcaagggcg
     6841 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     6901 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     6961 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     7021 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     7081 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     7141 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     7201 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     7261 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     7321 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     7381 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     7441 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     7501 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagtaatt ctagagtcgg
     7561 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     7621 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     7681 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     7741 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     7801 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacctcg accaaggcag
     7861 ctgtagatct tagccatctg ctttttgctt gtactgtcga ccctgtggaa tgtgtgtcag
     7921 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7981 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8041 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     8101 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8161 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8221 ggaggcctag gcttttgcaa aaagcttacc atggtgagca agggcgagga ggataacatg
     8281 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     8341 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     8401 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     8461 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     8521 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     8581 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     8641 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     8701 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     8761 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     8821 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     8881 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     8941 atggacgagc tgtacaagta acagatcggc gtgatggaag agtaaagcgg ccgcgaaagg
     9001 agcgcacgac cccatgcatc gatactagtt aaaagttttg ttactttata gaagaaattt
     9061 tgagtttttg ttttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat
     9121 tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac
     9181 ctcgatatac agaccgataa aacacatgcg tcaattttac gcatgattat ctttaacgta
     9241 cgtcacaata tgattatctt tctagggtta atctagtata cgcgtatgcg gccgcttaat
     9301 taatccatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     9361 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccctgaca
     9421 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     9481 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     9541 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     9601 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9661 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9721 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9781 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9841 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9901 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9961 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
    10021 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    10081 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    10141 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    10201 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    10261 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    10321 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    10381 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10441 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10501 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10561 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10621 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10681 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10741 ctgagcgtca gaccccgtag aaa
//
