LOCUS       sequence-454029-        9586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pyl aaRS/tRNA system for mammalian TAA suppression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Osgood AO, Zheng Y, Roy SJS, Biris N, Hussain M, Loynd C, Jewel D, 
            Italia JS, Chatterjee A
  TITLE     An Efficient Opal-Suppressor Tryptophanyl Pair Creates New Routes 
            for Simultaneously Incorporating up to Three Distinct Noncanonical 
            Amino Acids into Proteins in Mammalian Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2023 May 2;62(19):e202219269. doi: 
            10.1002/anie.202219269. Epub 2023 Apr 12.
  PUBMED    36905325
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl."; date: "2023-05-2"; volume: "62(19):e202219269. doi"; 
            pages: " 10.1002/anie.202219269. Epub 2023 Apr 12"
COMMENT     Sequence Label: pAcBac1-Pyl-TAA
FEATURES             Location/Qualifiers
     source          1..9586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..471
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(104..123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     313..334
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        497..601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(602..670,671..1462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(820..839)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1633..2221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2122..2141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2375..2392
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2526..2750
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2817..3350)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3539..3544,3545..3561,3562..3567))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    3997..4221
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4210..4227
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    4276..4864
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4382..4393
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4791..4811
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      6143..6152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6171..6190)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6172..6190)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6235..6438)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6264..6284)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6439..6742
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(6880..7128)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6938..6957)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7108..7128)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        7260..7351
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     7276..7293
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(7410..7457,7458..8945)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    9091..9225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9128..9147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9182..9201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  9254..9419
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
       61 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      121 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      181 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      241 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      301 acgttcttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      361 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      421 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
      481 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      541 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      601 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      661 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      721 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      781 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      841 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      901 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      961 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1021 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1081 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1141 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1201 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1261 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1321 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1381 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1441 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1501 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1561 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1621 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1681 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1741 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1801 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1861 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1921 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1981 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2041 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2101 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2161 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2221 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2281 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2341 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2401 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga
     2461 ccagccgcgt aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa
     2521 gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat
     2581 aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa
     2641 agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc
     2701 ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt
     2761 gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag
     2821 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
     2881 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
     2941 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
     3001 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
     3061 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     3121 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     3181 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     3241 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     3301 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
     3361 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     3421 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
     3481 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
     3541 tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt
     3601 gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa
     3661 cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta
     3721 cggcaaggtg ctgtgcacgg atctgccctt gcttcaggag atcggtagac ctcggccgtc
     3781 gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga
     3841 aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtac
     3901 ccgtagtggc tatggcaggg cttgcgctta atgcgccgct acagggcgcg tggggatacc
     3961 ccctagagcc ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag
     4021 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     4081 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     4141 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     4201 gctggcaact agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctctagact
     4261 cgagttaaag tcgacgcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa
     4321 ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg
     4381 gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac
     4441 aggcgagcag ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc
     4501 ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg
     4561 caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc
     4621 aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg
     4681 gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag
     4741 ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa
     4801 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
     4861 gattgtcgac ttaacgcgtt gaattcttac agattggttg aaatcccatt atagtaagat
     4921 tcggaccttg atgccctctt aatgttttta aagccgtgca taaccttgag caagcgttca
     4981 agaccaaaac ctgcacctat ccatggtttg tcaatacccc attctctatc aagagaaact
     5041 ggcccgacga ctgccgaaga aagctccagg tccccgtgca ttatatcaag agtatcccca
     5101 aagaccatac aggaatctcc tacgatttcg aagtcgattt ccagatagtc cagaaactct
     5161 ttgatgagag cttcaagatt ttcccgagta catcccgaac ccatctgaca gaagttcacc
     5221 atagtaaatt cttccaggtg ctctttgccg tcagactctt tccggtaaca aggtccgact
     5281 tcgaaaattt ttattgggcc tggtaaaatc ctatcgagtt ttcgcagata gttgtaaaga
     5341 gtcggggcaa gcattggcct caagcagaga tttttatcca cccggaagat ctgttttgaa
     5401 agttcagtat cattattaat acccattctc tccacgtatt ccgccggaat aaggatagga
     5461 gactttatct ccagaaaacc ccggtctacg aaaaatttcg taatatcacg ttcgagttta
     5521 ccgaggtagt cttctctatc attggtatag agccgctgaa aatcgttttt tcttcttgtc
     5581 acaagttcag gctcaagttc cctgaaaggc tttgccatat ttagagaaat tttatcctct
     5641 ggacttaaga gagcctcaac cctatcaagc tggcttcttg taagtgaagg agcaggagcc
     5701 gatgcgggaa cagacgaatt tggagttgat tttgcaggcg aaggtacaga tctggatgtg
     5761 ttcgttgatg cctttgcaga aacagaattt tccagaggct tcggagccct tgaaactgat
     5821 ttcggcatag cttttttgac ctttggagca gaaactaccc taactttcac actgtttttg
     5881 ctttcggttg atcttgtgag aaaattattg atatcctcgt ccgaaaccct acatcgtttg
     5941 caggtttttc tgtacttatg atgtctgaat gctctggctg ttctacaact cctggaatta
     6001 ttcacaacaa gatggtctcc acacgccatt tcaatgtata ttttacttct tgagacctca
     6061 tggtgcttga ttttgtggag cgtgccagtc ctggacatcc agagcccggt cgcagatatt
     6121 aaaacatcta atggtttttt atccatggtg gcgctagcca gcttgggtct ccctatagtg
     6181 agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct
     6241 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     6301 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     6361 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     6421 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     6481 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     6541 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     6601 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     6661 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     6721 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     6781 aattgattac tattaataac tagcaaaaaa cgggcagccc gggaatctaa cccggctgaa
     6841 cggatttaaa gtccgttcga tctacatgat cagccgcccg gtgtttcgtc ctttccacaa
     6901 gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat
     6961 tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt
     7021 attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct ctctaacagc
     7081 cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctt cctgcccgag
     7141 gtacccctag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
     7201 gcaaaacgga tcctgcaggt atttgcggcc gcggtccgta tactccggaa tattaataga
     7261 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     7321 taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat
     7381 cgggcgcgaa ctcctaaaaa accgccacca tgaagtgcct tttgtactta gcctttttat
     7441 tcattggggt gaattgcaag ttcaccatag tttttccaca caaccaaaaa ggaaactgga
     7501 aaaatgttcc ttctaattac cattattgcc cgtcaagctc agatttaaat tggcataatg
     7561 acttaatagg cacagcctta caagtcaaaa tgcccaagag tcacaaggct attcaagcag
     7621 acggttggat gtgtcatgct tccaaatggg tcactacttg tgatttccgc tggtatggac
     7681 cgaagtatat aacacattcc atccgatcct tcactccatc tgtagaacaa tgcaaggaaa
     7741 gcattgaaca aacgaaacaa ggaacttggc tgaatccagg cttccctcct caaagttgtg
     7801 gatatgcaac tgtgacggat gccgaagcag tgattgtcca ggtgactcct caccatgtgc
     7861 tggttgatga atacacagga gaatgggttg attcacagtt catcaacgga aaatgcagca
     7921 attacatatg ccccactgtc cataactcta caacctggca ttctgactat aaggtcaaag
     7981 ggctatgtga ttctaacctc atttccatgg acatcacctt cttctcagag gacggagagc
     8041 tatcatccct gggaaaggag ggcacagggt tcagaagtaa ctactttgct tatgaaactg
     8101 gaggcaaggc ctgcaaaatg caatactgca agcattgggg agtcagactc ccatcaggtg
     8161 tctggttcga gatggctgat aaggatctct ttgctgcagc cagattccct gaatgcccag
     8221 aagggtcaag tatctctgct ccatctcaga cctcagtgga tgtaagtcta attcaggacg
     8281 ttgagaggat cttggattat tccctctgcc aagaaacctg gagcaaaatc agagcgggtc
     8341 ttccaatctc tccagtggat ctcagctatc ttgctcctaa aaacccagga accggtcctg
     8401 ctttcaccat aatcaatggt accctaaaat actttgagac cagatacatc agagtcgata
     8461 ttgctgctcc aatcctctca agaatggtcg gaatgatcag tggaactacc acagaaaggg
     8521 aactgtggga tgactgggca ccatatgaag acgtggaaat tggacccaat ggagttctga
     8581 ggaccagttc aggatataag tttcctttat acatgattgg acatggtatg ttggactccg
     8641 atcttcatct tagctcaaag gctcaggtgt tcgaacatcc tcacattcaa gacgctgctt
     8701 cgcaacttcc tgatgatgag agtttatttt ttggtgatac tgggctatcc aaaaatccaa
     8761 tcgagcttgt agaaggttgg ttcagtagtt ggaaaagctc tattgcctct tttttcttta
     8821 tcatagggtt aatcattgga ctattcttgg ttctccgagt tggtatccat ctttgcatta
     8881 aattaaagca caccaagaaa agacagattt atacagacat agagatgaac cgacttggaa
     8941 agtgataagg ccaggccggc caagcttgtc gagaagtact agaggatcat aatcagccat
     9001 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     9061 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     9121 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9181 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag
     9241 cctaggagat ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
     9301 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
     9361 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag
     9421 cggggcattt ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa
     9481 accgtcatct tcggctactt tttctctgtc acagaatgaa aatttttctg tcatctcttc
     9541 gttattaatg tttgtaattg actgaatatc aacgcttatt tgcagc
//
