LOCUS       sequence-454031-       10252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CROP-seq-puro-v2 with scaffold sequence removed for the insertion of
            CRISPRmap Opool with guide, scaffold and barcode sequences, using 
            mTurquoise2 as selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Gu J, Iyer A, Wesley B, Taglialatela A, Leuzzi G, Hangai S, Decker 
            A, Gu R, Klickstein N, Shuai Y, Jankovic K, Parker-Burns L, Jin Y, 
            Zhang JY, Hong J, Niu X, Costa JA, Pezet MG, Chou J, Snoeck HW, 
            Landau DA, Azizi E, Chan EM, Ciccia A, Gaublomme JT
  TITLE     Mapping multimodal phenotypes to perturbations in cells and tissue 
            with CRISPRmap.
  JOURNAL   Nat Biotechnol. 2024 Oct 7. doi: 10.1038/s41587-024-02386-x.
  PUBMED    39375448
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Oct 7. doi: 10.1038/s41587-024-02386-x."
COMMENT     Sequence Label: CROP-seq-mTurquoise2-v2-no-scaffold
FEATURES             Location/Qualifiers
     source          1..10252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1489..1606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1745..2923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1976..2914
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2871..2891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2930..2939
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2936..2938,2939..2941,2942..3655)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2981..3002)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3242..3261)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3589..3610
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3671..4259
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3724..3744)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4142..4153)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4415..4663
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4415..4435
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4586..4605
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             6591..6771
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6849..6970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6886..6905)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6940..6959
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7010..7145
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7165..7184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7166..7184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7194..7211)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7194..7210)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7203..7225)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7352..7807
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7439..7458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7649..7670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7833..7937
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7938..8006,8007..8798)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8156..8175)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8969..9557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9458..9477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9711..9728
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9845..9866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9881..9886,9887..9904,9905..9911)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9919..9935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9924..9946
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9943..9959
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9943..9959
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9978..9998
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9980..9998
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10026..10252
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     1501 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1561 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1621 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa
     1681 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     1741 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1801 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     1861 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1921 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1981 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2041 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     2101 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     2161 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     2221 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     2281 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     2341 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     2401 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     2461 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     2521 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2581 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2641 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     2701 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     2761 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2821 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2881 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg ccaccatggt
     2941 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3001 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3061 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3121 gaccaccctg tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     3181 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3241 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3301 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3361 ggagtacaac tactttagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     3421 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     3481 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3541 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3601 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaacgcg
     3661 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3721 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3781 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3841 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3901 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3961 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4021 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4081 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4141 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4201 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     4261 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4321 aaggggggac tggaagggct aattcactcc caacgaagac aagatttcac cattatcgtt
     4381 tcagacccac ctcccaaccc cgaggggacc cagagagggc ctatttccca tgattccttc
     4441 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
     4501 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     4561 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     4621 cgatttcttg gctttatata tcttgtggaa aggacgaaac accggagacg gttgtaaatg
     4681 agcacacaaa atacacatgc taaaatatta tattctatga cctttataaa atcaaccaaa
     4741 atcttctttt taataacttt agtatcaata attagaattt ttatgttcct ttttgcaaac
     4801 ttttaataaa aatgagcaaa ataaaaaaac gctagtttta gtaactcgcg ttgttttctt
     4861 cacctttaat aatagctact ccaccacttg ttcctaagcg gtcagctcct gcttcaatca
     4921 ttttttgagc atcttcaaat gttctaactc caccagctgc tttaactaaa gcattgtctt
     4981 taacaactga cttcattagt ttaacatctt caaatgttgc acctgatttt gaaaatcctg
     5041 ttgatgtttt aacaaattct aatccagctt caacagctat ttcacaagct ttcatgattt
     5101 cttcttttgt taataaacaa ttttccataa tacatttaac aacatgtgat ccagctgctt
     5161 tttttacagc tttcatgtct tctaaaacta attcataatt tttgtctttt aatgcaccaa
     5221 tatttaatac catatcaatt tctgttgcac catctttaat tgcttcagaa acttcgaatg
     5281 cttttgtagc tgttgtgcat gcacctagag gaaaacctac aacatttgtt attcctacat
     5341 ttgtgccttt taataattct ttacaatagc ttgttcaata tgaattaaca caaactgttg
     5401 caaaatcaaa ttcaattgct tcatcacata attgtttaat ttcagctttc gtagcatctt
     5461 gttttaataa tgtgtgatct atatatttgt ttagtttcat tttttctcct atatattcat
     5521 ttttaatttt aattctttaa taatttcgtc tactttaact ttagcgtttt gaacagattc
     5581 accaacacct ataaaataaa tttttagttt aggttcagtt ccacttgggc gaacagcaaa
     5641 tcatgactta tcttctaaat aaaattttag taagtcttgt cctggcatat tatacattcc
     5701 atcgatgtag tcttcaacat taacaacttt aagtccagca atttgagtta agggtgttgc
     5761 tctcaatgat ttcattaatg gttcaatttt taatttcttt tcttctggtt taaaattcaa
     5821 gtttaaagtg aaagtgtaat atgcacccat ttctttaaat aaatcttcta aatagtctac
     5881 taatgtttta ttttgttttt tataaaatca agcagcctct gctattaata tagaagcttg
     5941 tattccatct ttatctctag ctgagtcatc aattacatat ccataacttt cttcataagc
     6001 aaaaacaaaa tttaatccgt tatcttcttc tttagcaatt tctctaccca ttcatttaaa
     6061 tccagttaaa gtttttacaa tattaactcc atatttttca tgagcgattc tatcacccaa
     6121 atcacttgtt acaaaacttg aatatagagc cggatttttt ggaatgctat ttaagcgttt
     6181 tagatttgat aattttcaat caattaaaat tggtcctgtt tgatttccat ctaatcttac
     6241 aaaatgacca tcatgtttta ttgccattcc aaatctgtca gcatctgggt cattcataat
     6301 aataatatct gcatcatgtt taataccata ttcaagcggt atttttcatg caggatcaaa
     6361 ttctggattt ggatttacaa catttttaaa tgtttcatct tcaaatgcat gctcttcaac
     6421 ctcaataacg ttatatcctg attcacgtaa tatttttggg gtaaatttag ttcctgttcc
     6481 attaactgcg ctaaaaataa tttttaaatc ttttttagct tcttgctctt ttttgtacgt
     6541 ctctaagctt ggcgtaacta gatcttgaga cactgctttt tgcttgtact gggtctctct
     6601 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6661 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6721 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     6781 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6841 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6961 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     7021 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7081 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7141 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     7201 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7261 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7321 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     7381 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7441 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7501 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7561 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7621 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7681 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7741 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7801 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7861 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7921 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7981 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8041 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8101 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8161 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8221 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8281 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8341 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8401 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8461 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8521 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8581 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8641 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8701 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8761 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8821 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8881 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8941 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9001 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9061 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9361 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9541 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9601 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9661 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9721 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9781 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9841 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9901 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9961 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    10021 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
    10081 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
    10141 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
    10201 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa ac
//
