LOCUS       sequence-454069-        2985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0, CDS module (AATG-GCTT) containing rice OsL5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu 
            CH
  TITLE     Development of a tightly regulated copper-inducible transient gene 
            expression system in Nicotiana benthamiana incorporating suicide 
            exon and Cre recombinase
  JOURNAL   bioRxiv 2024.03.23.586378
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.23.586378"
COMMENT     Sequence Label: pCHW100002
FEATURES             Location/Qualifiers
     source          1..2985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     84..103
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1448..2239
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2580..2985,1..183)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
       61 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      121 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      181 aaaaacgcca gcaacgcggc ctttttacgg ttcctgcact ctgtggtctc aaatgggagg
      241 gtttgtcaag acccagaaaa ccaatgccta ccacaagcgt ttccaagtca agttcaagag
      301 aaggaggcag ggcaagactg actacagggc caggattagg ctcaccagcc aagataagaa
      361 caagtacaac acaccgaagt accgctttgt tgtgagattt acaaacaaag atatcacagc
      421 tcaaattgtc tatgctacca ttgcgggtga tatcgtgatg gctgctgcct actcccatga
      481 gctgcctcgt tatggtcttg aagttggtct taccaactat gcggcagctt actgcactgg
      541 cttgcttctg gctcgccgtg tgctcacgct ccgtggtttg gaccaggagt acgagggcaa
      601 tgttgaggcc actggggagg actactatgt tgaaccagct gatgaaagga ggcctttccg
      661 tgctctcttg gatgttggcc tcattaggac aaccactgga aaccgtgtct ttggtgccct
      721 caagggagct ttggatggtg gtcttgacat tcctcacagt gacaagaggt ttgctgggtt
      781 caagaaggac gagaagcagc ttgattctga tattcaccgc aagtacatct acggtgggca
      841 cgtggctgac tacatgaggt ctatggctga ggaggaacct gagaaattcc aagcccactt
      901 tagtgagtac ctcaagaagg gaattgacgc tgatggcatg gaatcactgt acaagaaggt
      961 ccatgctgcc atccgcgctg atcctaccat ggccaaatcg accaagaagg aaccggctac
     1021 ccacaagaga tacaacctca agaagctaac ctacgagcag aggaaggcca gtctcgtcga
     1081 aagattgaat gctctcaact cctcagctgg tgctgatgat gatgacgagg aagaggatga
     1141 tgagtgagct tcgagaccac gaagtgccga ggcacgaacc cagtggacat aagcctgttc
     1201 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     1261 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     1321 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     1381 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     1441 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     1501 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     1561 ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga ccgtaaggct
     1621 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     1681 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     1741 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     1801 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     1861 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     1921 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     1981 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     2041 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     2101 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     2161 cttggcctcg cgcgcagatc agttggaaga atttgtccat tacgtgaaag gcgagatcac
     2221 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg
     2281 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
     2341 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
     2401 tatatcatgc tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2461 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2521 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2581 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2641 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2701 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2761 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2821 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2881 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2941 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgcc
//
