LOCUS       sequence-454077-       15887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 targeting vector containing an all-in-one DOX-inducible system
            for CRISPRa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15887)
  AUTHORS   Petazzi P, Ventura T, Luongo FP, McClafferty H, May A, Taylor HA, 
            Shipston MJ, Romano N, Forrester LM, Menendez P, Fidanza A
  TITLE     A novel human pluripotent stem cell gene activation system 
            identifies IGFBP2 as a mediator in the production of haematopoietic 
            progenitors in vitro.
  JOURNAL   Elife. 2024 Dec 23;13:RP94884. doi: 10.7554/eLife.94884.
  PUBMED    39714446
REFERENCE   2  (bases 1 to 15887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-12-23"; volume: "13:RP94884. doi"; pages: " 
            10.7554/eLife.94884"
COMMENT     Sequence Label: AAVS1-iSAM
FEATURES             Location/Qualifiers
     source          1..15887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2049..2054,2055..2072,2073..2079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2196..3032
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    3157..3175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3193..3211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3229..3247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3265..3283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3301..3319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3337..3355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3373..3391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3556..3576
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3586..3609,3610..3612,3613..6099,6100..6102,
                     6103..7686)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3586..7686
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7741..7761
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7786..7935
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7954..8010
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8023..8409
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             8464..8484
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8500..9042
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             9067..9438
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             9454..9507
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9508..10218
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMSFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9653..9671)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    10235..10283
                     /note="synthetic polyadenylation signal"
     polyA_signal    10921..11055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10958..10977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11012..11031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(11065..11811)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(11919..13101)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11928..12870
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(11951..11971)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    13363..13474
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     13463..13480
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(13496..14290)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13607..13626)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     14217..14236
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(14300..14353)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    14377..14402
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    14403..15206
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(15220..15239)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15221..15239)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15249..15266)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15249..15265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15258..15280)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      15407..15862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15494..15513)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15704..15725
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctccaccgc ggtggagagc agagccagga acccctgtag
     2221 ggaaggggca ggagagccag gggcatgaga tggtggacga ggaaggggga cagggaagcc
     2281 tgagcgcctc tcctgggctt gccaaggact caaacccaga agcccagagc agggccttag
     2341 ggaagcggga ccctgctctg ggcggaggaa tatgtcccag atagcactgg ggactcttta
     2401 aggaaagaag gatggagaaa gagaaaggga gtagaggcgg ccacgacctg gtgaacacct
     2461 aggacgcacc attctcacaa agggagtttt ccacacggac acccccctcc tcaccacagc
     2521 cctgccagga cggggctggc tactggcctt atctcacagg taaaactgac gcacggagga
     2581 acaatataaa ttggggacta gaaaggtgaa gagccaaagt tagaactcag gaccaactta
     2641 ttctgatttt gtttttccaa actgcttctc ctcttgggaa gtgtaaggaa gctgcagcac
     2701 caggatcagt gaaacgcacc agacagccgc gtcagagcag ctcaggttct gggagagggt
     2761 agcgcagggt ggccactgag aaccgggcag gtcacgcatc ccccccttcc ctcccacccc
     2821 ctgccaagct ctccctccca ggatcctctc tggctccatc gtaagcaaac cttagaggtt
     2881 ctggcaagga gagagatggc tccaggaaat gggggtgtgt caccagataa ggaatctgcc
     2941 taacaggagg tgggggttag acccaatatc aggagactag gaaggaggag gcctaaggat
     3001 ggggcttttc tgtcaccaat cctgtcccta gtagcggccc tagtgatcca agtataagac
     3061 aaaagtgttg tggaattgct ccaggcgatc tgacggttca ctaaacgagc tctgctttta
     3121 taggcgccca ccgtacacgc ctacctcgac atacgttctc tatcactgat agggagtaaa
     3181 ctggatatac gttctctatc actgataggg agtaaactgt agatacgttc tctatcactg
     3241 atagggagta aactggtcat acgttctcta tcactgatag ggagtaaact ccttatacgt
     3301 tctctatcac tgatagggag taaactggat atacgttctc tatcactgat agggagtaaa
     3361 ctgtagatac gttccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg
     3421 gcgcctataa aagcagagct cgtttagtga accgtcagat cgcctggagc aattccacaa
     3481 cacttttgtc ttatacttgg atcactaggg ccgctagctc tagagagcgt cgaattgtcg
     3541 acggccacca tgagccccaa gaagaagaga aaggtggagg ccagcgacaa gaagtacagc
     3601 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     3661 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     3721 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     3781 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     3841 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     3901 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     3961 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     4021 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     4081 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     4141 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     4201 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     4261 gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc cctgagcctg
     4321 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     4381 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     4441 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     4501 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     4561 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     4621 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     4681 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     4741 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     4801 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     4861 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     4921 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     4981 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     5041 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     5101 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     5161 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     5221 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     5281 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     5341 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     5401 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     5461 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     5521 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     5581 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     5641 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     5701 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     5761 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     5821 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     5881 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     5941 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     6001 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     6061 gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc tcagagcttt
     6121 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     6181 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     6241 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     6301 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     6361 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     6421 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     6481 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     6541 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     6601 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     6661 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     6721 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     6781 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     6841 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     6901 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     6961 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     7021 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     7081 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     7141 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     7201 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     7261 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     7321 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     7381 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     7441 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     7501 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     7561 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     7621 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     7681 ggcgacagcg ctggaggagg tggaagcgga ggaggaggaa gcggaggagg aggtagcgga
     7741 cctaagaaaa agaggaaggt ggcggccgct ggatccggac gggctgacgc attggacgat
     7801 tttgatctgg atatgctggg aagtgacgcc ctcgatgatt ttgaccttga catgcttggt
     7861 tcggatgccc ttgatgactt tgacctcgac atgctcggca gtgacgccct tgatgatttc
     7921 gacctggaca tgctgattaa cggaggaagc ggagctacta acttcagcct gctgaagcag
     7981 gctggagacg tggaggagaa ccctggacct ggaagcggaa tggcttcaaa ctttactcag
     8041 ttcgtgctcg tggacaatgg tgggacaggg gatgtgacag tggctccttc taatttcgct
     8101 aatggggtgg cagagtggat cagctccaac tcacggagcc aggcctacaa ggtgacatgc
     8161 agcgtcaggc agtctagtgc ccagaagaga aagtatacca tcaaggtgga ggtccccaaa
     8221 gtggctaccc agacagtggg cggagtcgaa ctgcctgtcg ccgcttggag gtcctacctg
     8281 aacatggagc tcactatccc aattttcgct accaattctg actgtgaact catcgtgaag
     8341 gcaatgcagg ggctcctcaa agacggtaat cctatccctt ccgccatcgc cgctaactca
     8401 ggtatctaca gcgctggagg aggtggaagc ggaggaggag gaagcggagg aggaggtagc
     8461 ggacctaaga aaaagaggaa ggtggcggcc gctggatccc cttcagggca gatcagcaac
     8521 caggccctgg ctctggcccc tagctccgct ccagtgctgg cccagactat ggtgccctct
     8581 agtgctatgg tgcctctggc ccagccacct gctccagccc ctgtgctgac cccaggacca
     8641 ccccagtcac tgagcgctcc agtgcccaag tctacacagg ccggcgaggg gactctgagt
     8701 gaagctctgc tgcacctgca gttcgacgct gatgaggacc tgggagctct gctggggaac
     8761 agcaccgatc ccggagtgtt cacagatctg gcctccgtgg acaactctga gtttcagcag
     8821 ctgctgaatc agggcgtgtc catgtctcat agtacagccg aaccaatgct gatggagtac
     8881 cccgaagcca ttacccggct ggtgaccggc agccagcggc cccccgaccc cgctccaact
     8941 cccctgggaa ccagcggcct gcctaatggg ctgtccggag atgaggactt ctcaagcatc
     9001 gctgatatgg actttagtgc cctgctgtca cagatttcct ctagtgggca gggaggaggt
     9061 ggaagcggct tcagcgtgga caccagtgcc ctgctggacc tgttcagccc ctcggtgacc
     9121 gtgcccgaca tgagcctgcc tgaccttgac agcagcctgg ccagtatcca agagctcctg
     9181 tctccccagg agccccccag gcctcccgag gcagagaaca gcagcccgga ttcagggaag
     9241 cagctggtgc actacacagc gcagccgctg ttcctgctgg accccggctc cgtggacacc
     9301 gggagcaacg acctgccggt gctgtttgag ctgggagagg gctcctactt ctccgaaggg
     9361 gacggcttcg ccgaggaccc caccatctcc ctgctgacag gctcggagcc tcccaaagcc
     9421 aaggacccca ctgtctccgc tagcggcagt ggagagggca gaggaagtct gctaacatgc
     9481 ggtgacgtcg aggagaatcc tggcccaatg gtgagcaagg gcgaggagga taacatggcc
     9541 atcatcaagg agttcatgag cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     9601 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     9661 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     9721 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     9781 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     9841 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     9901 accaacttcc cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc
     9961 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
    10021 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
    10081 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
    10141 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
    10201 gacgagctgt acaagtagtg ataaaagcgg ccgcaataaa atatctttat tttcattaca
    10261 tctgtgtgtt ggttttttgt gtgcccgagc ttcctcgtcg acgctcactt aattaaggcg
    10321 cgcctgtcat tctaaatctc tctttcagcc taaagctttt tccccgtatc cccccaggtg
    10381 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
    10441 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
    10501 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
    10561 ctgggggctt tgggaggggg ctgtccccgt gagctcccag gcgcgcctgt cattctaaat
    10621 ctctctttca gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag
    10681 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
    10741 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
    10801 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg
    10861 gggctgtccc cgtgagctcc caggcgcgcc ttaattaatg actcgctgcg ctcggtattt
    10921 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10981 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11041 tatcatgtct ggatccttac ttagttaccc ggggagcatg tcaaggtcaa aatcgtcaag
    11101 agcgtcagca ggcagcatat caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc
    11161 taagtcaaaa tcgtcaaggg cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc
    11221 caggccacat atgattagtt ccaggccgaa aaggaaggca ggttcggctc cctgccggtc
    11281 gaacagctca attgcttgtt tcagaagtgg gggcatagaa tcggtggtag gtgtctctct
    11341 ttcctctttt gctacttgat gctcctgttc ctccaatacg cagcccagtg taaagtggcc
    11401 cacggcggac agagcgtaca gtgcgttctc cagggagaag ccttgctgac acaggaacgc
    11461 gagctgattt tccagggttt cgtactgttt ctctgttggg cgggtgccga gatgcacttt
    11521 agccccgtcg cgatgtgaga ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa
    11581 gtcttgccat gactcgcctt ccagggggca ggagtgggta tgatgcctgt ccagcatctc
    11641 gattggcagg gcatcgagca gggcccgctt gttcttcacg tgccagtaca gggtaggctg
    11701 ctcaactccc agcttttgag cgagtttcct tgtcgtcagg ccttcgatac cgactccatt
    11761 gagtaattcc agagcagagt ttatgacttt gctcttgtcc agtctagaca tggtgaattc
    11821 ggggccgcgg aggctggatc ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg
    11881 gcgtctccag gcgatctgac ggttcactaa acgagctctc acgacacctg aaatggaaga
    11941 aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg
    12001 gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt ctccacccac
    12061 tcagtgtggg gaaactccat cgcataaaac ccctcccccc aacctaaaga cgacgtactc
    12121 caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt ggagtcacat
    12181 gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc acccgcccgc
    12241 tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg aagcggccat
    12301 ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg cggggcgata
    12361 cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta cccccgtccg
    12421 attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg ggacgcacgg
    12481 gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc ttggcccgca
    12541 tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa attttaaatg
    12601 gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat tcgcacgcgg
    12661 cggccccagc gcccaagcca ggcctcaact caagcacgag gcgaaggggc tccttaagcg
    12721 caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag aatcacgtac
    12781 tgcagccagg ggcgtggaag taattcaagg cacgcaaggg ccataacccg taaagaggcc
    12841 aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa
    12901 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
    12961 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
    13021 ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc
    13081 ccactgacgg gcaccggagc ctcacgcatg ctcttctcca cctcagtgat gacgagagcg
    13141 ggcgggtgag ggggcgggaa cgcagcgatc tctgggttct acgttagtgg gagtttaacg
    13201 acggtccctg ggattcccca aggcaggggc gagtcctttt gtatgaatta ctcgatatca
    13261 tgagaattct agcaccggtt gctattgtct tcccaatcct cccccttgct gtcctgcccc
    13321 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
    13381 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
    13441 caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg ggttttcaga
    13501 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
    13561 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
    13621 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
    13681 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
    13741 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
    13801 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
    13861 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
    13921 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
    13981 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
    14041 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
    14101 cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
    14161 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
    14221 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
    14281 gttcagccat ttcgagccta gggccgggat tctcctccac gtcaccgcat gttagaagac
    14341 ttcctctgcc ctctccgctg ccagatctct cgaggccctg tgggaggaag agaagaggtc
    14401 aggccccact gtggggtgga ggggacagat aaaagtaccc agaaccagag ccacattaac
    14461 cggccctggg aatataaggt ggtcccagct cggggacaca ggatccctgg aggcagcaaa
    14521 catgctgtcc tgaagtggac ataggggccc gggttggagg aagaagacta gctgagctct
    14581 cggacccctg gaagatgcca tgacaggggg ctggaagagc tagcacagac tagagaggta
    14641 aggggggtag gggagctgcc caaatgaaag gagtgagagg tgacccgaat ccacaggaga
    14701 acggggtgtc caggcaaaga aagcaagagg atggagaggt ggctaaagcc agggagacgg
    14761 ggtactttgg ggttgtccag aaaaacggtg atgatgcagg cctacaagaa ggggaggcgg
    14821 gacgcaaggg agacatccgt cggagaaggc catcctaaga aacgagagat ggcacaggcc
    14881 ccagaaggag aaggaaaagg gaacccagcg agtgaagacg gcatggggtt gggtgaggga
    14941 ggagagatgc ccggagagga cccagacacg gggaggatcc gctcagagga catcacgtgg
    15001 tgcagcgccg agaaggaagt gctccggaaa gagcatcctt gggcagcaac acagcagaga
    15061 gcaaggggaa gagggagtgg aggaagacgg aacctgaagg aggcggcagg gaaggatctg
    15121 ggccagccgt agaggtgacc caggccacaa gctgcagaca gaaagcggca caggcccagg
    15181 ggagagaatg ctggtcagag aaagcaggta cccaattcgc cctatagtga gtcgtattac
    15241 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    15301 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    15361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    15421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    15481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    15541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    15601 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    15661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    15721 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    15781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    15841 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtg
//
