LOCUS       sequence-454094-        2368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 
            promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu 
            CH
  TITLE     Development of a tightly regulated copper-inducible transient gene 
            expression system in Nicotiana benthamiana incorporating suicide 
            exon and Cre recombinase
  JOURNAL   bioRxiv 2024.03.23.586378
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.23.586378"
COMMENT     Sequence Label: pCHW110032
FEATURES             Location/Qualifiers
     source          1..2368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      341..929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     830..849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1577..2368
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
       61 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
      121 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg ctgtcagacc
      181 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
      241 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
      301 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
      361 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      421 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      481 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      541 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      601 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      661 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      961 tgcactctgt ggtctcagga gagtggtctt gggagagcga tgcgtctttt ccgatgaacc
     1021 gttccagcaa aaaagactaa gcgatgcgtc ttttccgctg aaccgttcca gcaaaaaaga
     1081 ctaagcgatg cgtcttttcc gctgaaccgt tccagcaaaa aagactagga gagcgatgcg
     1141 tcttttccgc tgaaccgttc cagcaaaaaa gactatccct actgtttatt tacaagagcg
     1201 tacggttcaa tccctgcctc ccctgtaaaa ctaccctttg aaaacctctc tttcttaatc
     1261 ttttctttgt aattccaatg cgagaccacg aagtgccgag gcacgaaccc agtggacata
     1321 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     1381 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     1441 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     1501 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     1561 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     1621 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     1681 ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg ttacggtgac
     1741 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     1801 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     1861 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     1921 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     1981 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     2041 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     2101 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     2161 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     2221 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     2281 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt acgtgaaagg
     2341 cgagatcacc aaggtagtcg gcaaataa
//
