LOCUS       sequence-454096-        3206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0, N-terminal tag module (CCAT-AATG) containing 
            mCherry2-HyP5SM..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu 
            CH
  TITLE     Development of a tightly regulated copper-inducible transient gene 
            expression system in Nicotiana benthamiana incorporating suicide 
            exon and Cre recombinase
  JOURNAL   bioRxiv 2024.03.23.586378
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.23.586378"
COMMENT     Sequence Label: pCHW100003
FEATURES             Location/Qualifiers
     source          1..3206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2158..2949
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctgcactc tgtggtctca
      721 ccatggtgag taaaggagaa gaaaacaact tagctatcat taaagagttc atgcgcttca
      781 aagttcacat ggagggttct gttaacggtc acgagttcga gatcgaaggc gaaggcgagg
      841 gccgtccgta tgaaggcacc cagaccgcca aactgaaagt gactaaaggc ggcccgctgc
      901 cttttgcgtg ggacatcctg agcccgcaat ttatgtacgg ttctaaagcg tatgttaaac
      961 acccagcgga tatcccggac tatctgaagc tgtcttttcc ggaaggtttc aactgggagg
     1021 tagatttatg catcctcttg tcatgagaag tcgaattgtt cccattctgt gtgttgcagc
     1081 tacagatgga gatacataga gatactcgtg gattttgctt agtgttgagt tttgttctgg
     1141 ttgtgaacta aaagtttata catttgcagg aaataaatag ccttttgttt aaatcaaaag
     1201 gtcttaccta tgttagtgtg aagcattgga tcccaaagag agaactccaa aatgcgatga
     1261 ggcatattta atcttgtctg gactagtaac aggttgggat gaccacctgt gaagctccaa
     1321 caggattgcc tcctcacgca atgtttgagg tctgatgttc aatagcttgt tttgtttcac
     1381 tttgctttgg actttctttt cgccaatgag ctatgtttct gatggttttc actcttttgg
     1441 tgtgtagaga gtaatgaatt ttgaagatgg tggtgtcgtg accgtcactc aggactcctc
     1501 cctgcaggat ggcgagttca tctataaagt taaactgcgt ggtactaatt ttccatctga
     1561 tggcccggtg atgcagtgta ggacgatggg ttgggaggcg tctaccgaac gcatgtatcc
     1621 ggaagatggt gcgctgaaag gcgaaattaa acagcgcctg aaactgaaag atggcggcca
     1681 ttatgacgct gaagtgaaaa ccacgtacaa agccaagaaa cctgtgcagc tgcctggcgc
     1741 gtacaatgtg gatattaaac tggacatctt atctcataat gaagattata cgatcgtaga
     1801 gcaatatgag cgcgcggagg gtcgtcattc taccggtggc atggatgaac tatacaaaat
     1861 gcgagaccac gaagtgccga ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     1921 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1981 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     2041 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     2101 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     2161 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     2221 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     2281 ctgaagccac acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     2341 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     2401 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     2461 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     2521 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     2581 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     2641 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2701 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2761 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2821 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     2881 cgcgcagatc agttggaaga atttgtccat tacgtgaaag gcgagatcac caaggtagtc
     2941 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     3001 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     3061 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     3121 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3181 aaaggatcta ggtgaagatc cttttt
//
